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Biological Interaction Between Human Gingival Fibroblasts and Vascular Endothelial Cells for Angiogenesis: A Co-culture Perspective

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  • Published:
Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Advancement in cell culture protocols, multidisciplinary research approach, and the need of clinical implication to reconstruct damaged or diseased tissues has led to the establishment of three-dimensional (3D) test systems for regeneration and repair. Regenerative therapies, including dental tissue engineering, have been pursued as a new prospect to repair and rebuild the diseased/lost oral tissues. Interactions between the different cell types, growth factors, and extracellular matrix components involved in angiogenesis are vital in the mechanisms of new vessel formation for tissue regeneration. In vitro pre-vascularization is one of the leading scopes in the tissue-engineering field. Vascularization strategies that are associated with co-culture systems have proved that there is communication between different cell types with mutual beneficial effects in vascularization and tissue regeneration in two-dimensional or 3D cultures. Endothelial cells with different cell populations, including osteoblasts, smooth muscle cells, and fibroblasts in a co-culture have shown their ability to advocate pre-vascularization. In this review, a co-culture perspective of human gingival fibroblasts and vascular endothelial cells is discussed with the main focus on vascularization and future perspective of this model in regeneration and repair.

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Acknowledgements

This review was supported by Universiti Sains Malaysia through the Research University Grant (RU-1001/PPSG/812168).

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Authors and Affiliations

  1. School of Dental Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia

    Nasar Um Min Allah, Zurairah Berahim, Azlina Ahmad & Thirumulu Ponnuraj Kannan

  2. Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia

    Thirumulu Ponnuraj Kannan

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  1. Nasar Um Min Allah
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  2. Zurairah Berahim
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  3. Azlina Ahmad
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  4. Thirumulu Ponnuraj Kannan
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Correspondence to Zurairah Berahim.

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The authors have no financial conflicts of interest.

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There are no animal experiments carried out for this review article.

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Um Min Allah, N., Berahim, Z., Ahmad, A. et al. Biological Interaction Between Human Gingival Fibroblasts and Vascular Endothelial Cells for Angiogenesis: A Co-culture Perspective. Tissue Eng Regen Med 14, 495–505 (2017). https://doi.org/10.1007/s13770-017-0065-y

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  • DOI: https://doi.org/10.1007/s13770-017-0065-y

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