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In vitro anti-inflammatory, antioxidant and antibacterial activities of the Algerian species Micropus bombycinus

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Abstract

The present study reports the in vitro pharmacological properties of the methanolic extract obtained from the species Micropus bombycinus Lag. The quantification of total phenolic content was performed using the Folin-Ciocalteu method. The antioxidant activity was evaluated by four different assays. Moreover, the in vitro anti-inflammatory activity was assessed using the albumin denaturation method and the antibacterial activity was estimated by agar disk diffusion assay against ten bacterial strains. The Micropus bombycinus methanolic extract contains several classes of secondary metabolites known for their biological properties. A high content of polyphenols was estimated (115.32 ± 0.47 μg GAE/ mg of plant extract). The MeOH extract possessed strong antioxidant activities in various assays that subsequently correlated with its phenolic content. It had also the ability to inhibit thermally-induced protein denaturation with a percentage of 27.51% at the concentration of 400 μg/mL. Furthermore, this extract showed antibacterial activity against all the tested strains with zones of inhibition ranging from 9 to 28 mm. It can be concluded that Micropus bombycinus could be an interesting source for potential bioactive compounds as inhibitors of pathogenic bacteria, and it helps to cure chronic inflammatory (Rheumatoid arthritis) and oxidative stress-related diseases.

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References

  • Abakar MH, Mahamat HH (2012) Properties and antibiotic susceptibility of Bacillus anthracis isolates from humans, cattle and tabanids, and evaluation of tabanid as mechanical vector of anthrax in the Republic of Chad. Jordan J Biol Sci 147:1–5

    Google Scholar 

  • Achika JI, Arthur DE, Gerald I, Adedayo A (2014) A review on the phytoconstituents and related medicinal properties of plants in the Asteraceae family. IOSR-JAC 7:1–8

    Article  CAS  Google Scholar 

  • Adaramola B, Onigbinde A (2016) Effect of extraction solvent on the phenolic content, flavonoid content and antioxidant capacity of clove bud. J Pharm Biol Sci 11:33–38

    Google Scholar 

  • Adnan L, Osman A, Abdul Hamid A (2011) Antioxidant activity of different extracts of Red Pitaya (Hylocereus polyrhizus) seed. Int J Food Prop 14:1171–1181

    Article  CAS  Google Scholar 

  • Angelotti F, Parma A, Cafaro G, Capecchi R, Alunno A, Puxeddu I (2017) One year in review 2017: pathogenesis of rheumatoid arthritis. Clin Exp Rheumatol 35:368–378

    PubMed  Google Scholar 

  • Christiaens E, Hansen W, Moinet J (1987) Isolement des expectorations d’un patient atteint de leucémie lymphoïde chronique et de broncho-emphysème d’une Enterobacteriaceae nouvellement décrite. Med Maladies Infect 17:732–734

    Article  Google Scholar 

  • De Beer D, Joubert E, Gelderblom WCA, Manley M (2017) Phenolic compounds: a review of their possible role as in vivo antioxidants of wine. S Afr J Enol Vitic 23:48–61

    Google Scholar 

  • From C, Hormazabal V, Granum PE (2007) Food poisoning associated with pumilacidin-producing Bacillus pumilus in rice. Int J Food Microbiol 115:319–324

    Article  CAS  PubMed  Google Scholar 

  • Fujiwara T, Katakura K, Ohira H (2019) Rheumatoid arthritis and gastrointestinal tract lesions (NSAID ulcers, amyloidosis). Gastrointestinal and Hepatic Manifestations of Rheumatic Diseases. Springer, Singapore, pp 97–121

    Chapter  Google Scholar 

  • Halliwell B, Gutteridge JM (2015) Free radicals in biology and medicine. Oxford University Press, USA

    Book  Google Scholar 

  • Hasan UH (2018) Antiarthritic efficacy of Clematis orientalis. Bangladesh J Pharmacol 13:142–148

    Article  Google Scholar 

  • Hida T, Minami M, Kubo Y (2017) Bilateral leg ulcers secondary to dystrophic calcinosis in a patient with rheumatoid arthritis. J Med Investig 64:308–310

    Article  Google Scholar 

  • Kalam S, Gul MZ, Singh R, Ankati S (2015) Free radicals: Implications in etiology of chronic diseases and their amelioration through nutraceuticals. Pharmacologia 6:11–20

    Article  Google Scholar 

  • Kaur R, Dogra NK (2014) A review on traditional uses, chemical constituents and pharmacology of Ageratum conyzoides L. (Asteraceae). Int J Phaemaceutical Biol Sci Arch 5:33–45

    Google Scholar 

  • Kim J, Kim HY, Song SY, Go S, Sohn HS, Baik S, Hyeon T (2019) Synergistic oxygen generation and reactive oxygen species scavenging by Manganese Ferrite/Ceria co-decorated nanoparticles for rheumatoid arthritis treatment. ACS Nano 13:3206–3217

    Article  CAS  PubMed  Google Scholar 

  • Kobayashi T, Murayama K, Ohta Y, Kawasaki N, Toyoshima S, Ishii-watabe A (2017) The safety profiles of biological drugs for rheumatoid arthritis. Jap J Pharmacoepidemiol 21:63–76

    Article  Google Scholar 

  • Matsukura H, Katayama K, Kitano N, Kobayashi K, Kanegane C, Higuchi A, Kyotani S (1996) Infective endocarditis caused by an unusual Gram-negative rod, Rahnella aquatilis. Pediatr Cardiol 17:108–111

    Article  CAS  PubMed  Google Scholar 

  • Monte J, Abreu A, Borges A, Simões L, Simões M (2014) Antimicrobial activity of selected phytochemicals against Escherichia coli and Staphylococcus aureus and their biofilms. Pathogens 3:473–498

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mothana RAA, Lindequist U (2005) Antimicrobial activity of some medicinal plants of the island Soqotra. J Ethnopharmacol 96:177–181

    Article  PubMed  Google Scholar 

  • Mouffouk C, Hambaba L, Haba H, Mouffouk S, Bensouici C, Mouffouk S, Khadraoui H (2018) Acute toxicity and in vivo anti-inflammatory effects and in vitro antioxidant and anti-arthritic potential of Scabiosa stellata. Orient Pharm Exp Med 18:335–348

    Article  CAS  Google Scholar 

  • Mouffouk C, Hambaba L, Haba H, Mouffouk S, Bensouici C (2020a) Evaluation of cytotoxic effect, anti-cholinesterase, hemolytic and antibacterial activities of the Species Scabiosa stellata L. Curr Bioact Comp 16:76–82

    Google Scholar 

  • Mouffouk S, Mouffouk C, HabaH BC (2020b) In vitro cytotoxic effect, hemolytic and antioxidant activities of the Algerian species Nonea vesicaria Rchb. Curr Bioact Comp. https://doi.org/10.2174/1573407216666200109120431

    Article  Google Scholar 

  • Niño J, Narváez DM, Mosquera OM, Correa YM (2006) Antibacterial, antifungal and cytotoxic activities of eight Asteraceae and two Rubiaceae plants from Colombian biodiversity. Braz J Microbiol 37:566–570

    Article  Google Scholar 

  • O’Dell JR (1999) Is there a role for antibiotics in the treatment of patients with rheumatoid arthritis? Drugs 57:279–282

    Article  PubMed  Google Scholar 

  • Phaniendra A, Jestadi DB, Periyasamy L (2014) Free radicals: properties, sources, targets, and their implication in various diseases. Indian J Clin Biochem 30:11–26

    Article  PubMed  PubMed Central  Google Scholar 

  • Pretorius E, Akeredolu OO, Soma P, Kell DB (2017) Major involvement of bacterial components in rheumatoid arthritis and its accompanying oxidative stress, systemic inflammation and hypercoagulability. Exp Biol Med 242:355–373

    Article  CAS  Google Scholar 

  • Quezel P, Santa S (1963) New flora of Algeria and the meridional desert regions. Tome II. Paris. In, French National Center for Scientific Research, pp 890–893

    Google Scholar 

  • Quiñonez-Flores CM, González-Chávez SA, Del Río ND, Pacheco-Tena C (2016) Oxidative stress relevance in the pathogenesis of the rheumatoid arthritis: a systematic review. Biomed Res Int 13:1–14

    Article  Google Scholar 

  • Sunmathi D, Sivakumar R, Ravikumar K (2016) In vitro anti-inflammatory and antiarthritic activity of ethanolic leaf extract of Alternanthera sessilis (L.) R. BR. ex DC and Alternanthera philoxeroides (Mart.) Griseb. Int J adv Pharm Biol Chem 5:109–115

    CAS  Google Scholar 

  • Tash K (2005) Rahnella aquatilis bacteremia from a suspected urinary source. J Clin Microbiol 43:2526–2528

    Article  PubMed  PubMed Central  Google Scholar 

  • Tena D, Martinez-Torres JA, Perez-Pomata MT, Saez-Nieto JA, Rubio V, Bisquert J (2007) Cutaneous infection due to Bacillus pumilus: Report of 3 cases. Clin Infect Dis 44(4):40–42

    Article  Google Scholar 

  • Zhao H, Zhang H, Yang S (2014) Phenolic compounds and its antioxidant activities in ethanolic extracts from seven cultivars of Chinese jujube. Food Sci Hum Wellness 3:183–190

    Article  Google Scholar 

Download references

Acknowledgements

The authors wish to express thanks to the DGRSDT of the Algerian Ministry of Higher Education and Scientific Research for PRFU Project (B00L01UN050120180001).

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Authors and Affiliations

  1. Laboratoire de Biologie Moléculaire et Cellulaire (LBMC), Département de Microbiologie, Faculté Des Sciences, Université Mentouri, Ain Elbye, Constantine 1, Algérie

    Samia Dekkiche

  2. Laboratoire de Chimie et Chimie de l’Environnement (LCCE), Département de Chimie, Faculté Des Sciences de la Matière, Université Batna 1, 05000, Batna, Algérie

    Chaima Mouffouk, Soumia Mouffouk & Hamada Haba

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  1. Samia Dekkiche
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  2. Chaima Mouffouk
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Correspondence to Hamada Haba.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Samia Dekkiche has no conflict of interest. Chaima Mouffouk has no conflict of interest. Soumia Mouffouk has no conflict of interest. Hamada Haba has no conflict of interest.

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Dekkiche, S., Mouffouk, C., Mouffouk, S. et al. In vitro anti-inflammatory, antioxidant and antibacterial activities of the Algerian species Micropus bombycinus. ADV TRADIT MED (ADTM) 21, 609–617 (2021). https://doi.org/10.1007/s13596-020-00506-x

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