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Chemical composition and antimicrobial activity of Hyptis suaveolens Poit. seed oil from Uttarakhand State, India

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Abstract

The present study was carried out to identify the chemical composition and antimicrobial activity of Hyptis suaveolens Poit. seed oil from Kalagarh region (Uttarakhand State, India). Gas Chromatograph Mass Spectrum (GC-MS) analysis of the seed oil suggested high amounts (86.96 %) of unsaturated fatty acids: linoleic acid (76.13 %), oleic acid (10.83 %) compared to saturated fatty acids i.e. palmitic acid (6.55 %), stearic acid (4.56 %) and heptacosanoic acid (1.94 %) as the main constituents. The oil (1 mg/ml to 0.125 mg/ml) was detrimental to the growth of bacterial and fungal strains tested. Among them Salmonella typhi MTCC 531 (9.92 ± 0.08 mm), Pseudomonas aeruginosa MTCC 424 (10.22 ± 0.11 mm), Lactobacillus plantarum MTCC 2621 (10.92 ± 0.09 mm) and Candida tropicalis MTCC 229 (8.11 ± 0.07 mm) were susceptible at lower concentration (0.125 %). Escherichia coli MTCC 443, Shigella flexnerii MTCC 1457 and Vibrio vulnificus MTCC 1145 were sensitive only at higher concentrations (0.5 and 1 %) tested. The bioactivity of 1 mg/ml seed oil against C. tropicalis MTCC 229 was comparable to Fluconazole control (25 μg). On the contrary Enterococcus fecalis MTCC 439 and Streptococcus fecalis MTCC 3378 were resistant to the seed oil treatment. Minimum Inhibition Concentrations (MIC) were found to be 0.125 mg/ml for S. typhi, and P. aeruginosa, L. plantarum and C. tropicalis, while it was 0.2 mg/ml for L. leishmanii, S. aureus and C. albicans. The observations indicate that the H. suaveolens seed oil possess appreciable antimicrobial properties against Gram negative bacteria tested and can be further studied for the mode of action and further lead as a natural antibiotic.

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References

  • Adda C, Atachi P, Hell K, Tamò M (2011) Potential use of the bushmint, Hyptis suaveolens, for the control of infestation by the pink stalk borer, Sesamia calamistis on maize in southern Benin, West Africa. J Insect Sci 11–33

  • Agoramoorthy M, Chandrasekaran V, Venkatesalu MJ (2007) Antibacterial and antifungal activities of fatty acid methyl esters of the blind-your-eye mangrove from India. Braz J Microbiol 38:739–742

    Article  Google Scholar 

  • Ajibaye OH, Okoh KN, Egbuna S, Afolabi BA, Iwalokun BO (2009) Insecticidal properties of Hyptis suaveolens on Anophelesgambiae mosquito: a preliminary report. Int J Malar Trop Dis 5:144–147

    Google Scholar 

  • AOAC (1990) Official methods of analysis, 14th edn. Association of Official Analytical Chemists, Washington, pp 801–805

    Google Scholar 

  • Babalola OO, Ojo OE, Oloyede FA (2011) Hepatoprotective activity of aqueous extract of the leaves of Hyptis suaveolens (L.) Poit. On acetaminophen induced hepatotoxicity in rabbits. Res J Chem Sci 1(7):85–88

    Google Scholar 

  • Bachheti RK, Indra R, Archana J, Rana V (2012) Physico-chemical study of seed oil of Prunus armeniaca L. grown in Garhwal region (India) and its comparison with some conventional food oils. Int Food Res J 19(2):577–581

    CAS  Google Scholar 

  • Basha SK, Sudarashanam G (2010) Ethnobotanical studies on medicinal plants used by sugalis of yerramalais in Kurnool district, Andhra Pradesh, India. Int J Phytomediation 2:349–353

    Google Scholar 

  • Bauer AW, Perry DM, Kirby WMM (1959) Single disc antibiotic sensitivity testing of Staphylococci. A.M.A. Arch Intern Med 104:208–216

    Article  CAS  Google Scholar 

  • Boonrod C, Tawatchai T, Chusri T, Chalermchai W (2012) Effect of Hyptis suaveolens Poit leaf extracts on seed germination and subsequent seedling growth of Pennsetum setosum (Swartz) L-C. Rich and Mimosa invisa Mart. Agric J 7(1):17–20

    Google Scholar 

  • Carlos VA, Leticia CA, Jesús A, Gerardo CH, Antonio MVM, Adelfo RR, María ESM (2012) Gastroprotection of Suaveolol, isolated from Hyptis suaveolens, against ethanol-induced gastric lesions in wistar rats: role of prostaglandins, nitric oxide and Sulfhydryls. Molecules 17:8917–8927

    Article  Google Scholar 

  • Chitra S, Patil MB, Kumar R (2009) Wound healing activity of hyptis suaveolens (L.) Poit. (Lamiaceae). Int J Pharm Technol Res 1:737–744

    Google Scholar 

  • Das PK, Sahoo S, Sethi R, Nayak PS, Nayak S, Joshi A (2009) Phytochemical and pharmacological investigation of the protective effect of plant Hyptis suaveolens against duodenal ulceration. J Glob Pharm Technol 1:82–87

    Google Scholar 

  • Dey SK, Banerjee D, Chattapadhyay S, Karmakar KB (2010) Antimicrobial activities of some medicinal plants of West Bengal. Int J Pharm Biol Sci 1(3):1–10

    Google Scholar 

  • Gavani U, Paarakh PM (2008) Antioxidant activity of Hyptis suaveolens Poit. Int J Pharm 4:227–229

    Article  Google Scholar 

  • Ghaffari H, Behrouz JG, Sampath KKK, Prakash HS (2011) Antioxidant properties of Hyptis suaveolens (L.) Poit. And characterization of a novel antioxidant compound. World Congr Biotechnol 524

  • Hisil Y (1988) Instrumental Analysis Technique Ege Univ. Engg. Fac. Publ. Nu.55, Izmir, Turkey

  • Iwalokun BA, Oluwadun A, Otunba A, Oyenuga OA (2012) Chemical composition and antimicrobial activity of a new Chemotype of Hyptis suaveolens (Poit) from Nigeria. Curr Res J Biol Sci 4(3):265–272

    CAS  Google Scholar 

  • Joshi S, Dewangan RP, Yadav S, Rawat DS, Pasha S (2012) Synthesis, antibacterial activity and mode of action of novel linoleic acid-dipeptide-spermidine conjugates. Org Biomol Chem 10(41):8326–35

    Article  CAS  PubMed  Google Scholar 

  • Kapoor RT (2011) Bioherbicidal potential of leaf residue of Hyptis suaveolens on the growth and physiological parameters of Parthenium hysterophorus L. Curr Res J Biol Sci 3:41–350

    Google Scholar 

  • Kodakandla VS, Guvvala V, Sabbu S, Bhukya B (2012) Variations in volatile oil compositions of different wild collections of Hyptis suaveolens (L.) Poit from Western Ghats of India. J Pharm 3(2):131–135

    Google Scholar 

  • Kumkum A, Ranjana V (2012) Inhibition of calcium oxalate crystallization in vitro by various extracts of Hyptis suaveolens (L.) Poit. Int Res J Pharm 3(3):261–264

    Google Scholar 

  • Maidment C, Dyson A, Beard J (2009) A study into measuring the antibacterial activity of lysozyme-containing foods. Nutr Food Sci 39(1):9–35

    Article  Google Scholar 

  • Morreira ACP, Oliveira LE, Wanderley PA, Carno ES, de Souza EL (2010) Chemical composition and antifungal activity of Hyptis suaveolens (Poit) leaves oil against Aspergillus species. Braz J Microbiol 41:28–33

    Article  Google Scholar 

  • Nayak SP, Nayak S, Kar DM, Das P (2010) In-vitro anthelmintic activity of whole plant extracts of Hyptis suaveolens Poit. Int J Curr Pharm Res 2(2):50–51

    CAS  Google Scholar 

  • Okukpe KM, Adeloye MA, Alli OI, Adeyina OA, Annongu AA (2012) Investigation of Phytohormonal potential of some selected tropical plants. Res J Med Plants 6(6):425–432

    Article  CAS  Google Scholar 

  • Penumetcha M, Song M, Merchant N, Parthasarathy S (2012) Pretreatment with n-6 PUFA protects against subsequent high fat diet induced atherosclerosis- potential role of oxidative stress-induced antioxidant defense. Atherosclerosis 220(1):53–8

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Raizada P (2006) Ecological and vegetative characteristics of a potent invader, Hyptis suaveolens Poit. from India. Lyonia 11(2):115–120

    Google Scholar 

  • Raja ARD, Jeeva S, Prakash JW, Johnson M, Irudayaraj V (2011) Antibacterial activity of selected ethnomedicinal plants from South India. Asian Pac J Trop Med 4(5):375–378

    Article  PubMed  Google Scholar 

  • Sathish V, Ravichandran VD, Usha G, Paarakh MP (2010) Antimicrobial studies on the extracts of Cocculus hirsutus Linn and Hyptis Suaveolens Poit. Indian J Nat Prod Resour 1:49–52

    Google Scholar 

  • Shaikat MZH, Taleb MH, Golam MA (2012) Phytochemical screening and Antidiarrhoeal Activity of Hyptis suaveolens. Int J Appl Res Nat Prod 5(2):1–4

    Google Scholar 

  • Shanti BM, Amit V, Alok M, Vijayakumar M (2011) Anti-hyperglycemic activity of leaves extract of Hyptis suaveolens L. Poit in streptozotocin induced diabetic rats. Asian Pac J Trop Med 4(9):689–693

    Article  Google Scholar 

  • Shashi A, Rawal S, Mahor A, Sabharwal M (2010) In Vitro evaluation on antilithiatic activity on Hyptis suaveolens (L.) leaves. Int J Plant Sci 1(12):85–87

    Google Scholar 

  • Sumathi S, Senthil KS, Bharathi V, Sahaya SS (2011) Antibacterial Activity of the plant extract of Symphytum officinale L. against selected pathogenic bacteria. Int J Res Pharm Sci 2(1):92–94

    Google Scholar 

  • Tonzibo ZF, Affia BF, Bedi G, Chalchat JC (2009) Chemical composition of essential oil of Hyptis Suaveolensis (L) Poit. from Côte d’Ivoire. Eur J Sci Res 38(4):565–571

    Google Scholar 

  • Wealth of India (1959) CSIR, New Delhi. V: 159

  • Zheng CJ, Yoo JS, Lee TG, Cho HY, Kim YH, Kim WG (2005) Fatty acid synthesis is a target for antibacterial activity of unsaturated fatty acids. FEBS Lett 579(23):5157–62

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The authors are thankful to Graphic Era University, Dehradun, Uttarakhand, India for providing the necessary facilities for the research work.

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We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Chemistry, Graphic Era University, Dehradun, Uttarakhand, India

    R. K. Bachheti, Indra Rai & Archana Joshi

  2. Department of Chemistry, College of Natural and Computational Sciences (CNCS), Haramaya University, P O Box 138, Ethiopia, North East Africa

    R. K. Bachheti

  3. Department of Biology, College of Natural and Computational Sciences (CNCS), Haramaya University, Ethiopia, North East Africa

    Archana Joshi & R. S. Satyan

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  1. R. K. Bachheti
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  2. Indra Rai
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Correspondence to R. K. Bachheti.

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Bachheti, R.K., Rai, I., Joshi, A. et al. Chemical composition and antimicrobial activity of Hyptis suaveolens Poit. seed oil from Uttarakhand State, India. Orient Pharm Exp Med 15, 141–146 (2015). https://doi.org/10.1007/s13596-015-0184-8

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