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Antioxidant and anticancer effect of methanolic extract of Aerva lanata Linn. against Ehrlich Ascites Carcinoma (EAC) in vivo

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Abstract

The present study was conducted to demonstrate the antioxidant and anticancer effects of methanol extract of the aerial parts of Aerva lanata Linn. In vitro antioxidant potentiality was tested using DPPH radical scavenging test, total phenol and flavonoid content and reducing power determination assays. The extracts showed moderate antioxidant activity in a dose dependent manner. In vivo anticancer activity was studied against Ehrlich Ascites Carcinoma (EAC) cells in Swiss albino mice by monitoring parameters like tumor weight measurement, survival time and tumor cell growth inhibition. It has been found that the compound at the dose of 40 mg/kg/day (i.p) significantly decreases tumor weight, increases life span and reduces tumor cell growth rate in comparison to those of EAC bearing mice receiving no extract. These results suggest that methanol extract of the aerial parts of Aerva lanata could be a source of natural anticancer and antioxidant compounds. In vitro cytotoxicity of the same extract was also assessed to link the finding with the potent anticancer activity of the experimental extract (LC50 less than 50 μg/ml).

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Acknowledgments

The authors thanks to Prof. Dr. Jahanara Khanom (Department of Biochemistry and Molecular Biology, University of Rajshahi, Bangladesh) for providing the EAC cells and also to Prof. Dr. Shaikh Bokhtear Uddin (Department of Botany, University of Chittagong, Bangladesh) for identification of the plant.

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Authors and Affiliations

  1. Department of Pharmacy, Jessore Science & Technology University, Jessore, 7408, Bangladesh

    Obayed Raihan

  2. Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh

    Afrina Brishti

  3. Department of Pharmacy, International Islamic University Chittagong, Chittagong, 4203, Bangladesh

    Entaz Bahar & Mominur Rahman

  4. Department of Biochemistry and Molecular biology, University of Rajshahi, Rajshahi, 6205, Bangladesh

    Forhadul Islam

  5. Department of Pharmacy, Southern University Bangladesh, 22, Shaheed Mirza Lane (East), Mehedibag Road, Chittagong, Bangladesh

    Syed Mohammed Tareq

  6. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka, Bangladesh

    Md. Aslam Hossain

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  1. Obayed Raihan
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  2. Afrina Brishti
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  3. Entaz Bahar
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  4. Forhadul Islam
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  5. Mominur Rahman
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  7. Md. Aslam Hossain
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Correspondence to Obayed Raihan.

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Raihan, O., Brishti, A., Bahar, E. et al. Antioxidant and anticancer effect of methanolic extract of Aerva lanata Linn. against Ehrlich Ascites Carcinoma (EAC) in vivo. Orient Pharm Exp Med 12, 219–225 (2012). https://doi.org/10.1007/s13596-012-0073-3

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  • DOI: https://doi.org/10.1007/s13596-012-0073-3

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