Abstract
Previous experimental evidence suggested that Bergenia ciliata (Saxifragaceae) and Terminalia chebula Retzius(Combretaceae) are effective against nephrolithiasis and have potential antioxidant activity. In this regard, hydro-methanolic extracts of B. ciliata rhizomes and T. chebula fruits were investigated for antioxidant potential against sodium oxalate induced oxidative imbalance in the kidney of female Wistar rats. We also performed molecular docking studies of all the reported phenolic compounds of both the plants to evaluate its interaction with the active site of oxalate binding protein (OBP). In summary, our findings showed that sodium oxalate caused significant increase in lipid peroxidation with concurrent decrease in activities of superoxide dismutase and catalase as well as in total reduced glutathione content in a concentration-dependent manner. The hydro-alcoholic extracts, however, when co-administered with sodium oxalate resulted in significant protection with maximum percent protection was achieved by B. ciliata. Thereafter, daucosterol showed best binding efficiency with OBP. However, further work on the purification of isolated bioactive components and pharmacological testing can reveal the therapeutic potential of the plant.
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Lieske, J. C., Pena de la Vega, L. S. & Slezak, J. M. Renal stone epidemiology in Rochester, Minnesota: An update. Kidney Int. 69, 760–764 (2006).
Trinchieri, A. Epidemiological trends in urolithiasis: Impact on our health care systems. Urol. Res. 34, 151–156 (2006).
Coe, F. L., Parks, J. H. & Asplin, J. R. The pathogenesis and treatment of kidney stones. N. Engl. J. Med. 327, 1141–1152 (1992).
Koul, H. et al. Activation of c-myc gene mediates the mitogenic effects of oxalate in LLC-PK1 cells, a line of renal epithelial cells. Kidney Int. 50, 1525(1996).
Koul, H. et al. Oxalate-induced initiation of DNA synthesis in LLC-PK1 cells, a line of renal epithelial cells. Biochem. Biophys. Res. Commun. 205, 632 (1994).
Thamilselvan, S., Khan, S. R. & Menon, M. Oxalate and calcium oxalate mediated free radical toxicity in renal epithelial cells: effect of antioxidants. Urol. Res. 31, 3 (2003).
Thamilselvan, S. & Khan, S. R. Oxalate and calcium oxalate crystals are injurious to renal epithelial cells: results of in vivo and in vitro studies. J. Nephrol. 11, 66 (1998).
Khand, F. D. et al. Mitochondrial superoxide production during oxalate-mediated oxidative stress in renal epithelial cells. Free Radical Biol. Med. 32, 1339 (2002).
Miller, C. et al. Oxalate toxicity in renal epithelial cells: characteristics of apoptosis and necrosis. Toxicol. Appl. Pharmacol. 162, 132 (2000).
Knoll, T. et al. The influence of oxalate on renal epithelial and interstitial cells. Urol. Res. 32, 304 (2004).
Knoll, T. Stone disease. Eur. Urol. Suppl. 6, 717–722 (2007).
Butterweck, V. & Khan, S. R. Herbal medicines in the management of urolithiasis: Alternative or complementary? Planta Med. 75, 1095–1103 (2009).
Prasad, K., Sujatha, D. & Bharathi, K. Herbal drugs in urolithiasis- a review. Phcog. Rev. 1, 175–179 (2007).
Cox, P. & Balick, M. The ethnobotanical approach to drug discovery. Sci. Am. 270, 82–87 (1994).
Pant, S., Samant, S. S. & Arya, S. C. Diversity and indigenous household remedies of the inhabitants surrounding Mornaula reserve forest in West Himalaya. Ind. J. Trad. Knowledge 8, 606–610 (2009).
Islam, M. et al. Bioactivity evaluation of Bergenia ciliata. Pak. J. Pharm. Sci. 15, 15–33 (2002).
Rajkumar, V., Guha, G., Kumar, A. R. & Mathew, L. Evaluation of antioxidant activities of Bergenia ciliata rhizome. Records Nat. Prod. 4, 38–48 (2010).
Singh, V. Traditional remedies to treat asthma in North West and Trans Himalayan region in J&K state. Fitoterapia 66, 507 (1995).
Saha, S. & Verma, R. J. Bergenia ciliata extract prevents ethylene glycol induced histopathological changes in the kidney. Acta Pol. Pharm. Drug Res. 68, 711–715 (2011).
Saha, S., Shrivastav, P. S. & Verma, R. J. Antioxidative mechanism involved in the preventive efficacy of Bergenia ciliata rhizomes against experimental nephrolithiasis in rats. Pharm. Biol. 52, 712–722 (2014).
Saha, S. & Verma, R. J. Inhibition of calcium oxalate crystallization in vitro by an extract of Bergenia ciliata. Arab J. Urol. 11, 187–192 (2013).
Tasaduq, S. A. et al. Hepatocurative and antioxidant profile of HP-1, a polyherbal phytomedicine. Human Exp. Toxicol. 22, 639–645 (2003).
Minkyun, N. A. et al. Cytoprotective effect on oxidative stress and inhibitory effect on cellular aging of Terminialia chebula fruit. Phytother. Res. 18, 737–741 (2004).
Kannan, V. R. et al. Anti-diabetic activity on ethanolic extracts of fruits of Terminalia chebula Retz. alloxan induced diabetic rats. Am. J. Drug Dis. Dev. 2, 135–142 (2012).
Lee, H. S., Jung, S. H., Yun, B. S. & Lee, K. W. Isolation of chebulic acid from Terminalia chebula Retz. & its antioxidant effect in isolated rat hepatocytes. Arch. Toxicol. 81, 211–218 (2007).
Lee, D. et al. Anti- bacterial and anti-viral activities of extracts from Terminalia chebula barks. J. Korean Soc. App. Biol. Chem. 54, 295–298 (2011).
Nair, V., Singh, S. & Gupta Y. K. Anti-arthritic and disease modifying activity of Terminalia chebula Retz. in experimental models. J. Pharm. Pharmacol. 62, 1806–1806 (2010).
Israni, D. A., Patel, K. V. & Gandhi, T. R. Anti-hyperlipidemic activity of aqueous extract of Terminalia chebula and Gaumutra in high cholesterol diet fed rats. Int. J. Pharm. Sci. 1, 48–59 (2010).
Pratibha, N. et al. Anti-inflammatory activities of Aller-7, a novel polyherbal formulation for allergic rhinitis. Int. J. Tissue Reac. 26, 43–51 (2004).
Thamilselvan, S., Hackett, R. L. & Khan, S. R. Lipid peroxidation in ethylene glycol induced hyperoxaluria and calcium oxalate nephrolithiasis. J. Urol. 157,1059–1063 (1997).
Moriyama, M. T. et al. Inhibitions of urinary oxidative stress and renal calcium level by an extract of Quercus salicina Blume/Quercus stenophylla Makino in a rat calcium oxalate urolithiasis model. Int. J. Urol. 16, 397–401 (2009).
Selvam, R. & Kurien, T. B. Induction of lipid peroxidation by oxalate in experimental rat urolithiasis. J. Biosci. 12, 367–373 (1987).
Green, M. L., Freel, R. W. & Hatch, M. Lipid peroxidation is not the underlying cause of renal injury in hyperoxaluric rats. Kidney Int. 68, 2629–2638 (2005).
Selvam, R., Kalaiselvi, P., Govindaraj, A., Murugan, V. M. & Satishkumar, A. S. Effect of Aerva lanata leaf extract and Vediuppu chunnam on the urinary risk factors of calcium oxalate urolithiasis during experimental hyperoxaluria. Pharmacol. Res. 43, 89–93 (2001).
Thamilselvan, S. & Menon, M. Vitamin E therapy prevents hyperoxaluria-induced calcium oxalate crystal deposition in the kidney by improving renal tissue antioxidant status. British J. Urol. Int. 96, 117–126 (2005).
Ghalayini, I. F., Al-Ghazo, M. A. & Harfeil, M. N. A. Prophylaxis and therapeutic effects of raspberry (Rubus idaeus) on renal stone formation in Balb/c mice. Int. Brazilian J. Urol. 37, 259–267 (2011).
Beyhan, O., Elmastas, M. & Gedikli, F. Total phenolic compounds and antioxidant capacity of leaf, dry fruit and fresh fruit of feijoa (Acca sellowiana, Myrtaceae). J. Med. Plants Res. 4, 1065–1072 (2010).
Toblli, J. E. et al. Effects of angiotensin II subtype 1 receptor blockade by losartan on tubulointerstitial lesions caused by hyperoxaluria. J. Urol. 168, 1550–1555 (2002).
Häckl, L. P., Cuttle, G., Dovichi, S. S., Lima-Landman, M. T. & Nicolau, M. Inhibition of angiotensin-converting enzyme by quercetin alters the vascular response to bradykinin and angiotensin I. Pharmacol. 65, 182–186 (2002).
Goretta, L. A., Ottaviani, J. I. & Fraga, C. G. Inhibition of angiotensin converting enzyme activity by flavanolrich foods. J. Agri. Food Chem. 54, 234–(2006).
Seethalakshmi, L., Selvam, R., Mahle, C. J. & Menon, M. Binding of oxalate to mitochondrial inner membranes of rat and kuman kidney. J. Urol. 135, 862–865 (1986).
Selvam, R. & Kalaiselvi, P. Oxalate binding proteins in calcium oxalate nephrolithiasis. Urol. Res. 31, 242–256 (2003).
Ohkawa, H., Ohishi, N. & Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem 95, 351–358 (1979).
Kakkar, P., Das, B. & Viswanathan, P. N. A modified spectrophotometric assay of superoxide dismutase. Ind. J. Biochem. Biophys. 21, 130–132 (1984).
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. Protein measurement with folin-phenol reagent. J. Biol. Chem. 193, 265–275 (1951).
Sinha, A. K. Calorimetric assay of catalase. Analytical Biochem. 47, 389–394 (1972).
Moron, M. S., Depierre, J. W. & Mannervik, B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochem. Biophys. Acta 582, 67–78 (1979).
Guex, N. & Peitsch, M. C. SWISS-MODEL and the Swiss-Pdb Viewer: An environment for comparative protein modeling. Electrophoresis 18, 2714–2723 (1997).
Gezelter, J. D. Introduction to Avogadro, Chemistry 20262- Computational Chemistry, Spring (2012).
Laurie, A. T. & Jackson, R. M. Q-SiteFinder: an energy-based method for the prediction of protein-ligand binding sites. Bioinform. 21, 1908–1916 (2005).
Trott, O. & Olson, A. J. AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading. J. Comput. Chem. 31, 455–461 (2010).
Huey, R., Morris, G. M., Olson, A. J. & Goodsell, D. S. A. Semiempirical free energy force field with charge based desolvation. J. Comput. Chem. 28, 1145–1152 (2007).
Solis, F. J. & Wets, J. B. Minimization by random search techniques. Math. Oper. Res. 6, 19–30 (1981).
Wallace, A. C., Laskowski, R.. & Thornton, J. M. LIGPLOT: A program to generate schematic diagrams of protein-ligand interactions. Prot. Eng. 8, 127–134 (1995).
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Saha, S., Verma, R.J. In vitro and in silico study of antioxidant effect of Bergenia ciliata and Terminalia chebula against sodium oxalate induced oxidative stress. Toxicol. Environ. Health Sci. 7, 50–57 (2015). https://doi.org/10.1007/s13530-015-0220-6
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DOI: https://doi.org/10.1007/s13530-015-0220-6