Skip to main content
Log in

Effect of coupling dewaxing and chemical pretreatment on valorization of waxy fruit wastes

  • Original Article
  • Published:
Biomass Conversion and Biorefinery Aims and scope Submit manuscript

Abstract

The improper management of underutilized tropical fruit wastes has led to severe environmental and health issues, particularly concerning PM2.5 and the greenhouse effect. Transforming these fruit wastes into value-added products is an innovative approach to mitigate its adverse impact on both the environment and human health. In this study, pomelo peel and banana peel were subjected to acid and alkaline pretreatment under the optimum conditions to enhance the sugar and ethanol yields. Both H2SO4 and NaOH pretreatment improved sugar yields from untreated pomelo (0.30 g sugar/g biomass) and banana peels (0.12 g sugar/g biomass). The highest yields obtained from NaOH-pretreated samples were increased by 2.56- and 3.19-fold times, respectively. Despite the enhanced sugar yield, the study also evaluated the effect of the dewaxing and washing process on the sugar yield. The study reveals that the sugar yield has further increased by incorporating the dewaxing and washing steps in the pretreatment process. Unwashed biomass after pretreatment has resulted in decreased sugar yield due to the presence of inhibitors. Furthermore, the study also evaluated the ethanol production from these biomasses, which confirms improved ethanol yield from the pretreated biomasses that were dewaxed and washed. The alkaline-pretreated, dewaxed, and washed pomelo peel and banana peel could produce 2.2- and 4.1-fold more ethanol than their untreated biomasses, respectively. Thus, this study provides valuable insight into the necessity of the dewaxing and washing process in the pretreatment for improved yield in the biorefining process.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Patel A, Shah AR (2021) Integrated lignocellulosic biorefinery: gateway for production of second generation ethanol and value added products. J Bioresour Bioprod 6:108–128. https://doi.org/10.1016/j.jobab.2021.02.001

    Article  Google Scholar 

  2. El-Ramady H, Brevik EC, Bayoumi Y, Shalaby TA, El-Mahrouk ME, Taha N, Elbasiouny H, Elbehiry F, Amer M, Abdalla N, Prokisch J, Solberg SØ, Ling W (2022) An overview of agro-waste management in light of the water-energy-waste nexus. Sustainability 14(23):15717. https://doi.org/10.3390/su142315717

    Article  Google Scholar 

  3. Cheok CY, Mohd Adzahan N, Abdul Rahman R, Zainal Abedin NH, Hussain N, Sulaiman R, Chong GH (2016) Current trends of tropical fruit waste utilization. Crit Rev Food Sci Nutr 58(3):335–361. https://doi.org/10.1080/10408398.2016.1176009

    Article  Google Scholar 

  4. Ahmad T, Danish M (2018) Prospects of banana waste utilization in wastewater treatment: a review. J Environ Manage 206:330–348. https://doi.org/10.1016/j.jenvman.2017.10.061

    Article  Google Scholar 

  5. Wang YJ, Li N, Ni GR, Zhou CH, Yin X, Huang HJ (2022) Recycling pomelo peel waste in the form of hydrochar obtained by microwave-assisted hydrothermal carbonization. Materials 15:9055. https://doi.org/10.3390/ma15249055

    Article  Google Scholar 

  6. Hoque ME, Rayhan AM, Shaily SI (2021) Natural fiber-based green composites: processing, properties and biomedical applications. Appl Sci Eng Prog 14(4):689–718. https://doi.org/10.14416/j.asep.2021.09.005

    Article  Google Scholar 

  7. Li P, Cai D, Luo Z, Qin P, Chen C, Wang Y, Zhang C, Wang Z, Tan T (2016) Effect of acid pretreatment on different parts of corn stalk for second generation ethanol production. Bioresour Technol 206:86–92. https://doi.org/10.1016/j.biortech.2016.01.077

    Article  Google Scholar 

  8. Williams CL, Emerson RM, Tumuluru JS (2017) Biomass compositional analysis for conversion to renewable fuels and chemicals, in: Biomass Volume Estimation and Valorization for Energy. InTech 251–270. https://doi.org/10.5772/65777

  9. Kádár Z, Schultz-Jensen N, Jensen JS, Hansen MAT, Leipold F, Bjerre AB (2015) Enhanced ethanol production by removal of cutin and epicuticular waxes of wheat straw by plasma assisted pretreatment. Biomass Bioenergy 81:26–30. https://doi.org/10.1016/j.biombioe.2015.05.012

    Article  Google Scholar 

  10. Lan Y (2019) Waxes, in: Encyclopedia of Food Chemistry. Elsevier 312–316. https://doi.org/10.1016/B978-0-08-100596-5.22344-5

  11. Gundupalli MP, Chuetor S, Cheenkachorn K, Rattanaporn K, Show PL, Cheng YS, Sriariyanun M (2021) Interferences of waxes on enzymatic saccharification and ethanol production from lignocellulose biomass. Bioengineering 8(11):171. https://doi.org/10.3390/bioengineering8110171

    Article  Google Scholar 

  12. Qi G, Peng F, Xiong L, Lin X, Huang C, Li H, Xinde CXC (2017) Extraction and characterization of wax from sugarcane bagasse and the enzymatic hydrolysis of dewaxed sugarcane bagasse. Prep Biochem Biotechnol 47:276–281. https://doi.org/10.1080/10826068.2016.1224246

    Article  Google Scholar 

  13. Chatkaew C, Panakkal EJ, Rodiahwati W, Kirdponpattara S, Chuetor S, Sriariyanun M, Cheenkachorn K (2021) Effect of sodium hydroxide pretreatment on released sugar yields from pomelo peels for biofuel production. E3S Web Conf. 302:02015. https://doi.org/10.1051/e3sconf/202130202015

    Article  Google Scholar 

  14. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31(3):426–428. https://doi.org/10.1021/ac60147a030

    Article  Google Scholar 

  15. Segal L, Creely JJ, Martin AE, Conrad CM (1959) An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer. Text Res J 29:786–794. https://doi.org/10.1177/004051755902901003

    Article  Google Scholar 

  16. Panakkal EJ, Cheenkachorn K, Chuetor S, Tantayotai P, Raina N, Cheng YS, Sriariyanun M (2022) Optimization of deep eutectic solvent pretreatment for bioethanol production from Napier grass. Sustain Energy Technol Assess 54:102856. https://doi.org/10.1016/j.seta.2022.102856

    Article  Google Scholar 

  17. Izzi A, Yusuf N, Ishak W, Kim H (2020) Box-Behnken-design based optimization strategy for alkaline pretreatment of palm oil mill effluent for producing reducing sugar. IOP Conf Ser Earth Environ Sci 596(1):012024. https://doi.org/10.1088/1755-1315/596/1/012024

    Article  Google Scholar 

  18. Rai P, Ashutosh P, Anjana P (2019) Optimization of sugar release from banana peel powder waste (BPPW) using Box-Behnken design (BBD): BPPW to biohydrogen conversion. Int J Hydrog Energy 44:25505–25513. https://doi.org/10.1016/j.ijhydene.2019.07.168

    Article  Google Scholar 

  19. Ramaraj R, Unpaprom Y (2019) Optimization of pretreatment condition for ethanol production from Cyperus difformis by response surface methodology. 3 Biotech 9:218. https://doi.org/10.1007/s13205-019-1754-0

    Article  Google Scholar 

  20. Pakchamni P, Afedzi AEK, Parakulsuksatid P (2022) Optimization of alkaline-assisted organosolv pretreatment of sugarcane trash for the production of succinic acid using response surface methodology. Biocatal Agric Biotechnol 43:102374. https://doi.org/10.1016/j.bcab.2022.102374

    Article  Google Scholar 

  21. Boontum A, Phetson J, Rodiawati W, Kitsubthawee K, Kuntothom T (2019) Characterization of diluted-acid pretreatment of water hyacinth. Appl Sci Eng Prog 12(4):253–263. https://doi.org/10.14416/j.asep.2019.09.003

    Article  Google Scholar 

  22. Yildirim O, Ozkaya B, Altinbas M, Demir A (2021) Statistical optimization of dilute acid pretreatment of lignocellulosic biomass by response surface methodology to obtain fermentable sugars for bioethanol production. Int J Energy Res 45:8882–8899. https://doi.org/10.1002/er.6423

    Article  Google Scholar 

  23. Kim I, Han JI (2012) Optimization of alkaline pretreatment conditions for enhancing glucose yield of rice straw by response surface methodology. Biomass Bioenergy 46:210–217. https://doi.org/10.1016/j.biombioe.2012.08.024

    Article  Google Scholar 

  24. Brienzo M, Ferreira S, Vicentim MP, de Souza W, Sant’Anna C (2014) Comparison study on the biomass recalcitrance of different tissue fractions of sugarcane culm. Bioenergy Res 7(4):1454–1465. https://doi.org/10.1007/s12155-014-9487-8

    Article  Google Scholar 

  25. Zhao J, Yang Y, Zhang M, Wang D (2021) Minimizing water consumption for sugar and lignin recovery via the integration of acid and alkali pretreated biomass and their mixed filtrate without post-washing. Bioresour Technol 337:125389. https://doi.org/10.1016/j.biortech.2021.125389

    Article  Google Scholar 

  26. Dharmalingam B, Tantayotai P, Panakkal EJ, Cheenkachorn K, Kirdponpattara S, Gundupalli MP, Cheng YS, Sriariyanun M (2023) Organic acid pretreatments and optimization techniques for mixed vegetable waste biomass conversion into biofuel production. Bioenergy Res 16:1667–1682. https://doi.org/10.1007/s12155-022-10517-y

    Article  Google Scholar 

  27. Rizal N, Ibrahim M, Zakaria M, Abd-Aziz S, Yee P, Hassan M (2018) Pre-treatment of oil palm biomass for fermentable sugars production. Mol 23:1381. https://doi.org/10.3390/molecules23061381

    Article  Google Scholar 

  28. Deshavath NN, Mohan M, Veeranki VD, Goud VV, Pinnamaneni SR, Benarjee T (2017) Dilute acid pretreatment of sorghum biomass to maximize the hemicellulose hydrolysis with minimized levels of fermentative inhibitors for bioethanol production. 3 Biotech 7:139. https://doi.org/10.1007/s13205-017-0752-3

    Article  Google Scholar 

  29. Wan G, Zhang Q, Li M, Jia Z, Guo C, Luo B, Wang S, Min D (2019) How pseudo-lignin is generated during dilute sulfuric acid pretreatment. J Agric Food Chem 67:10116–10125. https://doi.org/10.1021/acs.jafc.9b02851

    Article  Google Scholar 

  30. Liu Q, Li K, Liu CG, Zhao XQ, Bai FW, Sakdaronnarong C, Srinophakun P, Srinophakun TR (2020) Complex effects of wax on ionic liquid pretreatment of oil palm empty fruit bunch. Ind Crops Prod 152:112526. https://doi.org/10.1016/j.indcrop.2020.112526

    Article  Google Scholar 

  31. Ling Z, Chen S, Zhang X, Xu F (2017) Exploring crystalline-structural variations of cellulose during alkaline pretreatment for enhanced enzymatic hydrolysis. Bioresour Technol 224:611–617. https://doi.org/10.1016/j.biortech.2016.10.064

    Article  Google Scholar 

  32. Santos CC, de Souza W, Sant’Anna C, Brienzo M (2018) Elephant grass leaves have lower recalcitrance to acid pretreatment than stems, with higher potential for ethanol production. Ind Crops Prod 111:193–200. https://doi.org/10.1016/j.indcrop.2017.10.013

    Article  Google Scholar 

  33. Tan J, Li Y, Tan X, Wu H, Li H, Yang S (2021) Advances in pretreatment of straw biomass for sugar production. Front Chem 9:696030. https://doi.org/10.3389/fchem.2021.696030

    Article  Google Scholar 

  34. Gao D, Haarmeyer C, Balan V, Whitehead TA, Dale BE, Chundawat SP (2014) Lignin triggers irreversible cellulase loss during pretreated lignocellulosic biomass saccharification. Biotechnol Biofuels 7:175. https://doi.org/10.1186/s13068-014-0175-x

    Article  Google Scholar 

  35. Ummalyma SB, Baibhav K, Singh A (2022) Enzymatic digestibility of pretreated dewaxed bamboo residues as feedstock for bioethanol production. Syst Microbiol Biomanufacturing 2:716–722. https://doi.org/10.1007/s43393-022-00092-w

    Article  Google Scholar 

  36. Rajan K, Carrier DJ (2014) Effect of dilute acid pretreatment conditions and washing on the production of inhibitors and on recovery of sugars during wheat straw enzymatic hydrolysis. Biomass Bioenergy 62:222–227. https://doi.org/10.1016/j.biombioe.2014.01.013

    Article  Google Scholar 

  37. Zhao J, Yang Y, Zhang M, Wang D (2021) Effects of post-washing on pretreated biomass and hydrolysis of the mixture of acetic acid and sodium hydroxide pretreated biomass and their mixed filtrate. Bioresour Technol 339:125605. https://doi.org/10.1016/j.biortech.2021.125605

    Article  Google Scholar 

  38. Jönsson LJ, Martín C (2016) Pretreatment of lignocellulose: formation of inhibitory by-products and strategies for minimizing their effects. Bioresour Technol 199:103–112. https://doi.org/10.1016/j.biortech.2015.10.009

    Article  Google Scholar 

  39. Kim D (2018) Physico-chemical conversion of lignocellulose: inhibitor effects and detoxification strategies: a mini review. Molecules 23:309. https://doi.org/10.3390/molecules23020309

    Article  Google Scholar 

  40. Huong VTT, Atjayutpokin T, Chinwatpaiboon P, Smith SM, Boonyuen S, Luengnaruemitchai A (2022) Two-stage acid-alkali pretreatment of vetiver grass to enhance the subsequent sugar release by cellulase digestion. Renew Energ 195:755–765. https://doi.org/10.1016/j.renene.2022.06.069

    Article  Google Scholar 

  41. Sahoo D, Ummalyma SB, Okram AK, Pandey A, Sankar M, Sukumaran RK (2018) Effect of dilute acid pretreatment of wild rice grass (Zizania latifolia) from Loktak Lake for enzymatic hydrolysis. Bioresour Technol 253:252–255. https://doi.org/10.1016/j.biortech.2018.01.048

    Article  Google Scholar 

  42. Chen L, Li J, Lu M, Guo X, Zhang H, Han L (2016) Integrated chemical and multi-scale structural analyses for the processes of acid pretreatment and enzymatic hydrolysis of corn stover. Carbohydr Polym 141:1–9. https://doi.org/10.1016/j.carbpol.2015.12.079

    Article  Google Scholar 

  43. Song C-L, Othman JB (2022) Synthesis and characterization of lignin-incorporated carboxymethyl cellulose (CMC) films from oil palm lignocellulosic waste. Processes 10(11):2205. https://doi.org/10.3390/pr10112205

    Article  Google Scholar 

  44. Bouramdane Y, Fellak S, El Mansouri F, Boukir A (2022) Impact of natural degradation on the aged lignocellulose fibers of Moroccan cedar softwood: structural elucidation by infrared spectroscopy (ATR-FTIR) and X-ray diffraction (XRD). Fermentation 8(12):698. https://doi.org/10.3390/fermentation8120698

    Article  Google Scholar 

  45. Huang R, Cao M, Guo H, Qi W, Su R, He Z (2014) Enhanced ethanol production from pomelo peel waste by integrated hydrothermal treatment, multienzyme formulation, and fed-batch operation. J Agric Food Chem 62(20):4643–4651. https://doi.org/10.1021/jf405172a

    Article  Google Scholar 

  46. Bondesson PM, Galbe M, Zacchi G (2013) Ethanol and biogas production after steam pretreatment of corn stover with or without the addition of sulphuric acid. Biotechnol Biofuels 6:11. https://doi.org/10.1186/1754-6834-6-11

    Article  Google Scholar 

Download references

Acknowledgements

The authors would like to thank King Mongkut’s University of Technology North Bangkok (KMUTNB-67-KNOW-07) for providing financial support and all other facilities for conducting the research.

Funding

This work is financially supported by King Mongkut’s University of Technology North Bangkok (KMUTNB-67-KNOW-07).

Author information

Authors and Affiliations

Authors

Contributions

Kraipat Cheenkachorn and Chaichana Chatkaew: investigation, methodology, writing – original draft. Elizabeth Jayex Panakkal and Santi Chuetor: formal analysis and data curation. Hassan El Bari, Ponnusami Venkatachalam, and Pau-Loke Show: data curation and reviewing and editing, investigation, reviewing and editing; Malinee Sriariyanun: conceptualization, data curation, writing – reviewing and editing, funding acquisition, project administration.

Corresponding author

Correspondence to Malinee Sriariyanun.

Ethics declarations

Competing interests

The authors declare no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Cheenkachorn, K., Panakkal, E.J., Chatkaew, C. et al. Effect of coupling dewaxing and chemical pretreatment on valorization of waxy fruit wastes. Biomass Conv. Bioref. (2024). https://doi.org/10.1007/s13399-024-05501-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s13399-024-05501-9

Keywords

Navigation