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Producing high value aroma compounds by whole-cell biocatalysis using Aspergillus niger LBM055

  • Research Article-Chemical Engineering
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Abstract

The aims of this article were to optimize and assay the production of high value aroma compounds at a bench-scale by the bioconversion capacity of Aspergillus niger LBM055 strain using R-limonene as platform chemical. Temperature, pH and agitation effect on R-limonene bioconversion were evaluated. Optimal R-limonene bioconversion was met at 30.5 \(^{\circ }\hbox {C}\), 100 rpm and pH 6.2, meanwhile best yield of high molecular weights products was obtained at 28 \(^{\circ }\hbox {C}\), 100 rpm and pH 5.4. Conditions of best yield of high molecular weights were selected to apply them at a bench-scale bioreactor. \(\alpha \)-pinene, \(\beta \)-pinene, citral, linalool, carvone, carveol and myrcene were identified. Also, a mixture of carvone, carveol and limonene oxide at a high concentration of 3.1 g/L, was identified by GC-MS analysis. Optimization analysis demonstrated that with the same substrate and fungal strain, it was possible to obtain different bioaromas compounds only by changing the operating conditions. Applying the operating parameters in a bench-scale bioreactor, a composite biofragrance of a terpene mixture, recognized by their citric, woody, floral and minty aromatic notes, was obtained. This study contributed to identified operational condition for the R-limonene bioconversion scale-up for the extension in the citrus industry value chain.

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Availability of data and materials

Data that support the findings of this study are available in the Repositorio Institucional Digital de la Universidad Nacional de Misiones (RIDUNaM) at https://hdl.handle.net/20.500.12219/2998, Reference Number 20.500.12219/2998.

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Acknowledgements

P.D.Z., N.A.C. and M.A.S. are career members from CONICET. J.E.V. has a postdoctoral Grant from CONICET (RESOL-2021-1071-APN-DIR#CONICET), Argentina.

Funding

This research was funded by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Universidad Nacional de Misiones (UNaM) through the PIO CONICET-UNAM project (number 23720160100005CO).

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Authors and Affiliations

  1. Laboratorio de Biotecnología Molecular, Instituto de Biotecnología Misiones (InBioMis), Facultad de Ciencias Exactas, Químicas y Naturales (FCEQyN), Universidad Nacional de Misiones (UNaM), Ruta 12, km 7.5, 3300, Posadas, Misiones, Argentina

    Juan Velázquez, Marcela Sadañoski, Pedro Zapata & Laura Villalba

  2. Departamento de Ciencias Básicas, Área Química, Facultad de Ingeniería y Ciencias Agropecuarias, Instituto de Investigación en Tecnología Química (INTEQUI - CONICET), Universidad Nacional de San Luis, Almirante Brown 1455, CPD5730, Villa Mercedes, San Luis, Argentina

    Nora Comelli

  3. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290, C1425FQB, CABA, Buenos Aires, Argentina

    Juan Velázquez, Marcela Sadañoski, Pedro Zapata & Nora Comelli

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J.E.V. and M.A.S. wrote the main manuscript text, investigation and formal analysis, P.D.Z. worked in the resources and funding acquisition, and N.A.C and L.L.V supervised the work and edited the text. All authors reviewed the manuscript.

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Velázquez, J., Sadañoski, M., Zapata, P. et al. Producing high value aroma compounds by whole-cell biocatalysis using Aspergillus niger LBM055. Arab J Sci Eng 48, 16495–16506 (2023). https://doi.org/10.1007/s13369-023-08326-2

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