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Assessment of a diketopiperazine, cyclo(Trp-Phe) from Streptomyces griseoplanus SAI-25 against cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae)

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Abstract

Microorganisms produce a range of metabolites with varying pest control properties. With this concept, we earlier identified 15 Streptomyces spp. strains with insecticidal activity against Helicoverpa armigera (Hübner), Spodoptera litura (Fabricius), and Chilo partellus (Swinhoe). In recent studies, we evaluated an insecticidal compound purified from the extracellular extract of S. griseoplanus SAI-25 by bioactivity-guided fractionation against H. armigera. Spectral studies by infrared (IR), nuclear magnetic resonance (NMR), and electron spray ionization mass spectroscopy (ESI-MS) confirmed that the purified compound was cyclo(Trp-Phe) of the diketopiperazines class. Cyclo(Trp-Phe) exhibited antifeedant (70 %), larvicidal (67 %), and pupicidal (59 %) action against H. armigera in a dose-dependent manner. The lethal dose for 50 % of the group (LD50) and LD90 values for larvicidal effect were 619 and 2750 ppm, respectively. In addition, the purified compound prolonged larval (10.3–11.1 days) and pupal (10.9–11.8 days) periods compared with the untreated control (larval duration 9.8 days, pupal duration 10.6 days). This is the first report on the presence and biological activity of cyclo(Trp-Phe) isolated from the genus Streptomyces.

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Acknowledgments

The authors are grateful to the Department of Biotechnology, India, for their financial assistance (BT/PR13824/AGR/05/508/2010). This work was undertaken as part of the CGIAR Research Program on Grain Legumes. ICRISAT is a member of CGIAR Consortium. We thank the NMR research centre, IISc, Bangalore, India, and IIT, Madras, India, for NMR and ESI–MS analysis. We also thank ICRISAT and all of the staff members of the biocontrol unit, including P.V.S. Prasad, P. Manohar, B. Nagappa, D. Barath, and A. Jabbar, for technical assistance, and S. Suraj Prasad for larval supply.

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Authors and Affiliations

  1. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), 502 324, Patancheru, Telangana, India

    Arumugam Sathya, Rajendran Vijayabharathi, Bhimineni Ratna Kumari, Vadlamudi Srinivas, Hari C. Sharma & Subramaniam Gopalakrishnan

  2. Department of Biological Science and Technology, Bioinorganic and Molecular Imaging Laboratory, National Chiao Tung University, Hsinchu, Taiwan

    Palanisamy Sathyadevi

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  1. Arumugam Sathya
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  2. Rajendran Vijayabharathi
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Correspondence to Subramaniam Gopalakrishnan.

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Sathya, A., Vijayabharathi, R., Kumari, B.R. et al. Assessment of a diketopiperazine, cyclo(Trp-Phe) from Streptomyces griseoplanus SAI-25 against cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae). Appl Entomol Zool 51, 11–20 (2016). https://doi.org/10.1007/s13355-015-0366-3

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