Abstract
In vivo electroporation is a very powerful technology that enables both gain-of-function and loss-of-function analyses. However, the device required for the method is expensive. Here, we report a simple, inexpensive (under US$85), easily constructed, and one-piece electroporation device for gene functional analyses of insects. Using this simple electroporator, we successfully introduced exogenous fluorescence protein genes into three moth species. This simple low-cost electroporator will render gene function analyses accessible to many non-model insects, including pest species.
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Acknowledgments
We thank H. Fujiwara and H. Sezutsu for providing plasmids pPIG-A3GR and pHA3PIG, respectively; N. Konno for providing experimental equipment; Y. Ishikawa and T. Kayukawa for providing insect samples; M. Watanabe and K. Shinyashiki for technical assistance; and H. Matsuzawa, A. Fujiwara, M. Oida, A. Kurata, M. Takizawa and M. Wakabayashi for their helpful discussions. This work was supported by KAKENHI (grant number 22128007). T. S. was supported by a JSPS Research Fellowship for Young Scientists (grant number 13J10329).
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Sugimoto, T.N., Tsuchida, T. Simple electroporation device for gene functional analyses in insects. Appl Entomol Zool 50, 271–275 (2015). https://doi.org/10.1007/s13355-014-0315-6
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DOI: https://doi.org/10.1007/s13355-014-0315-6