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First report of ‘Candidatus Phytoplasma australasia’ related strain associated with opium poppy phyllody and stunting in India

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Abstract

During a survey performed in opium fields grown at Acharya Narendra Deva University of Agriculture & Technology (ANDUAT), Kumarganj, Ayodhya, Uttar Pradesh, India in March 2021, symptoms of phyllody and stunting were observed on opium plants. The association of phytoplasmas was confirmed in all the symptomatic opium samples by using PCR of 16S rRNA and secA genes sequences. Association of a ‘Candidatus Phytoplasma australasia’ related strain was confirmed by 16S rRNA and secA gene sequence comparison analysis. Virtual RFLP analysis of ~ 1.25 kb product of 16S rRNA gene sequences using the iPhyClassifier tool further classified the opium phytoplasma isolate into the 16SrII-D subgroup. This is the first report on identification of a phytoplasma disease in opium in world.

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Authors and Affiliations

  1. Department of Plant Pathology, Acharya Narendra Deva University of Agriculture & Technology, , Kumarganj, Ayodhya, Uttar Pradesh, India

    Sushil Kumar Singh & S. N. Rahul

  2. Indian Institute of Pulse Research, Kalyanpur, Kanpur, Uttar Pradesh, 208024, India

    R. K. Mishra

  3. Department of Plant Pathology, Sri Venkateswara Agricultural College, Acharya N. G. Ranga Agricultural University, Tirupati, Andhra Pradesh, 517502, India

    M. Gurivi Reddy

  4. Department of Pharmacy, University of Salerno, Fisciano, I-84084, Salerno, Italy

    Carmine Marcone

  5. Division of Plant Pathology, ICAR- Indian Agricultural Research Institute, New Delhi, 1100012, India

    Govind P. Rao

Authors
  1. Sushil Kumar Singh
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  2. R. K. Mishra
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  3. M. Gurivi Reddy
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  4. S. N. Rahul
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  5. Carmine Marcone
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  6. Govind P. Rao
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Corresponding author

Correspondence to Govind P. Rao.

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Singh, S.K., Mishra, R.K., Reddy, M.G. et al. First report of ‘Candidatus Phytoplasma australasia’ related strain associated with opium poppy phyllody and stunting in India. Australasian Plant Pathol. 52, 501–505 (2023). https://doi.org/10.1007/s13313-023-00937-5

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  • DOI: https://doi.org/10.1007/s13313-023-00937-5

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