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Human surfactant proteins A2 (SP-A2) and B (SP-B) genes as determinants of respiratory distress syndrome

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Abstract

Objective

To study the relationship between SP-A2 and SP-B gene polymorphisms and respiratory distress syndrome in preterm neonates.

Design

Cross-sectional.

Setting

Neonatal intensive care unit and the Molecular Biology unit of the Chemical Pathology Department, Kasr Alainy hospital, Cairo University.

Participants

Sixty-five preterm infants with respiratory distress syndrome and 50 controls. The genomic DNA was isolated using DNA extraction kits. SYBR Greenbased real-time PCR was used to determine the variant genotypes of SP-A2 c.751 G<A and SP-B c.8714 G<C single nucleotide polymorphisms.

Results

Homozygosity of SP-A (OR 46, 95% CI 14–151) and SP-B (OR 5.2, 95% CI 2.3–11.4) alleles increased the risk of respiratory distress syndrome. The logistic regression model showed that genotypes SP-A2 (OR 164) and SP-B (OR 18) were directly related to the occurrence of respiratory distress syndrome, whereas gestational age (OR 0.57) and 5-minute Apgar score (OR 0.19) were inversely related to its occurrence.

Conclusions

There is a possible involvement of SP-A2 and SP-B genes polymorphisms in the genetic predisposition to respiratory distress syndrome.

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Authors and Affiliations

  1. Departments of Pediatrics, Faculty of Medicine, Cairo University, Cairo, Egypt

    Walaa A. Abuelhamed, Nancy Zeidan & Walaa A. Shahin

  2. Departments of Public Health and Community Medicine, Faculty of Medicine, Cairo University, Cairo, Egypt

    Hoda I. Rizk

  3. Departments of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt

    Walaa A. Rabie

Authors
  1. Walaa A. Abuelhamed
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  2. Nancy Zeidan
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  3. Walaa A. Shahin
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  4. Hoda I. Rizk
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  5. Walaa A. Rabie
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Correspondence to Walaa A. Rabie.

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Abuelhamed, W.A., Zeidan, N., Shahin, W.A. et al. Human surfactant proteins A2 (SP-A2) and B (SP-B) genes as determinants of respiratory distress syndrome. Indian Pediatr 52, 391–394 (2015). https://doi.org/10.1007/s13312-015-0643-9

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  • DOI: https://doi.org/10.1007/s13312-015-0643-9

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