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High expression of Naa10p associates with lymph node metastasis and predicts favorable prognosis of oral squamous cell carcinoma

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Tumor Biology

Abstract

N-a-Acetyltransferase 10 protein (Naa10p) is a potential prognostic biomarker and a modulator of several types of cancer. Despite the efforts to elucidate the relationship between Naa10p expression and clinical prognosis, little is known about its expression and role in human oral squamous cell carcinoma (OSCC). In this study, we firstly detected the mRNA and protein levels of Naa10p in 10 paired OSCC tissue samples and found Naa10p was frequently overexpressed in the tumor tissues of patients with OSCC. Further detection by immunohistochemistry was used to examine Naa10p expression in 124 OSCC tumor specimens by tissue microarray (TMA), and a relative high level of Naa10p protein expression was found in 98 out of 124 cases (79.03 %). Additional analyses illustrated that Naa10p expression inversely correlated with clinical stage (p = 0.047), degree of lymph node status (p = 0.020), differentiation (p = 0.022), and recurrence (p = 0.016) of patients with OSCC. The survival analysis showed that patients with Naa10p-positive expression had a better prognosis for disease-free survival (DFS) or overall survival (OS) than those with Naa10p-negative expression (p = 0.003 for both). Furthermore, we assessed the effect of Naa10p knockdown on motility of oral cancer cells in vitro, and the results showed that Naa10p inhibit cell wound healing, migration, and invasion. In summary, our study illustrated that the expression of Naa10p had a potential value for predicting the progression of OSCC and prognosis of OSCC patients.

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Acknowledgments

This study was supported by a research grant from the National Natural Science Foundation of China (81560473; 81560442; 31160183; 30960093), Doctoral Foundation and Technology Research and Achievements Transformation Program of Xinjiang production and Construction Corps (2014BB021; 2015 AD003), and High Level Talents Special Foundation of Shihezi University, China (RCZX201330). We deeply appreciate Dr. Chengchao Shou (Peking University Cancer Institute) for generously sharing of anti-Naa10p monoclonal antibody.

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    Authors and Affiliations

    1. Department of Biochemistry and Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, Xinjiang, 832002, China

      Yan Zeng, Juan Zhao & Yang Yang

    2. Department of Stomatology, The First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, Xinjiang, 832002, China

      Jun Zheng, Pei-Rong Jia, Guo-Jun Yang & Jiang Xu

    3. Department of Radiation Oncology, College of Medicine, University of Florida, Gainesville, FL, 32611, USA

      Jing-Feng Ma

    4. Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing, 100850, China

      Yong-Qing Gu

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    1. Yan Zeng
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    Yan Zeng and Jun Zheng are considered joint first authors.

    Yan Zeng and Jun Zheng contributed equally to this work.

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    Zeng, Y., Zheng, J., Zhao, J. et al. High expression of Naa10p associates with lymph node metastasis and predicts favorable prognosis of oral squamous cell carcinoma. Tumor Biol. 37, 6719–6728 (2016). https://doi.org/10.1007/s13277-015-4563-z

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    • DOI: https://doi.org/10.1007/s13277-015-4563-z

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