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The SDF-1/CXCR4 chemokine axis in uveal melanoma cell proliferation and migration

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Tumor Biology

Abstract

The stromal-cell-derived factor 1 (SDF-1)/chemokine receptor 4 (CXCR4) chemokine axis plays a key role in tumor migration. Here, we analyzed the axis in uveal melanoma (UM) proliferation and migration and investigated the effect of a chemical inhibitor of CXCR4, AMD3100, on UM. We found that CXCR4 was expressed in all five UM cell lines tested as well as the retinal pigment epithelium cell line ARPE-19 cells, while CXCR7 was only detected in OM290 and VUP cell lines. SDF-1 promotes the proliferation and migration of OCM-1 and OCM431 cell lines, while AMD3100 weakens this function. Taken together, our results show that the SDF-1/CXCR4 chemokine axis plays a key role in UM cell proliferation and migration and that AMD3100 can alleviate this function, which may offer a hint for UM treatment.

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Acknowledgments

This work was supported by the Scientific Research Program of the National Health and Family Planning Commission of China (201402014), National Natural Science Foundation of China (81172323, 81372469, 31470757), Program for Professor of Special Appointment (Eastern Scholar) at the Shanghai Institutions of Higher Learning (1410000159), SMC-ChenXing Yong Scholar Program (2014, Class B), and Science and Technology Commission of Shanghai (14JC1404200, 14430723100, 13JC1406202). The funders did not have a role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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    Authors and Affiliations

    1. Department of Ophthalmology, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200011, China

      Jianjun Bi, Peng Li, Chuanyin Li, Jie He, Ying Wang, He Zhang, Xianqun Fan, Renbing Jia & Shengfang Ge

    2. Department of Biochemistry and Molecular Biology, Shanghai Jiaotong University School of Medicine, Shanghai, 200011, China

      Jianjun Bi, Peng Li, Chuanyin Li, Ying Wang, He Zhang & Shengfang Ge

    3. State Key Laboratory of Molecular Biology, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai, 200031, China

      Chuanyin Li

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    Jianjun Bi and Peng Li contributed equally to this work.

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    Bi, J., Li, P., Li, C. et al. The SDF-1/CXCR4 chemokine axis in uveal melanoma cell proliferation and migration. Tumor Biol. 37, 4175–4182 (2016). https://doi.org/10.1007/s13277-015-4259-4

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