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C-reactive protein inhibits expression of N-cadherin and ZEB-1 in murine colon adenocarcinoma

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Tumor Biology

Abstract

Epithelial-to-mesenchymal transition (EMT) is thought to play a key role in cancer cell invasion and metastasis. We previously demonstrated that cancer cell migration is inhibited by C-reactive protein (CRP), which is widely used as a biomarker of inflammation, though its functions are not fully understood. In the present study, we evaluated the effect of CRP on cancer cell migration and expression of mesenchymal and epithelial markers of EMT and of related transcription factors. MCA-38 murine colon adenocarcinoma cells were subcutaneously inoculated into the backs of C57BL/6 mice, which also received 1 μg of recombinant mouse CRP or vehicle (phosphate-buffered saline) subcutaneously every 3 days for 4 weeks. Thereafter, the mice were sacrificed for evaluation using quantitative real-time polymerase chain reaction (PCR) and immunohistochemistry. There was no statistical difference in tumor size between the control and CRP groups, but CRP dose-dependently inhibited MCA-38 cell migration. PCR analysis confirmed that CRP suppresses expression of N-cadherin (p < 0.01), a mesenchymal marker of EMT, and ZEB-1, an EMT-related transcription factor (p < 0.01). These findings suggest that CRP inhibits EMT in a MCA-38 tumor-bearing mouse model. CRP may thus be a potentially useful tool for preventing cancer progression through suppression of EMT.

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Acknowledgments

The authors wish to thank Ms. Mitsuko Sato and Ms. Jun Kodama for their secretarial support.

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Authors and Affiliations

  1. Department of Surgery, Division of Thoracic Surgery, Akita University Graduate School of Medicine, 1-1-1 Hondo, Akita City, 010-8543, Japan

    Satoshi Kudo, Hajime Saito, Satoru Motoyama, Tomohiko Sasaki, Kazuhiro Imai, Hayato Konno, Shinogu Takashima, Maiko Atari, Yusuke Sato & Yoshihiro Minamiya

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  1. Satoshi Kudo
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Correspondence to Satoshi Kudo.

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Kudo, S., Saito, H., Motoyama, S. et al. C-reactive protein inhibits expression of N-cadherin and ZEB-1 in murine colon adenocarcinoma. Tumor Biol. 36, 7035–7043 (2015). https://doi.org/10.1007/s13277-015-3414-2

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