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Prognostic significance of USP10 as a tumor-associated marker in gastric carcinoma

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Tumor Biology

Abstract

Ubiquitin-specific protease 10 (USP10), a novel deubiquitinating enzyme, had been associated with growth of tumor cell. However, the role of USP10 in gastric cancer carcinogenesis had not been elucidated yet. The aim of this study was to investigate the expression level of USP10 in gastric carcinoma (GC) tissues and cell lines, then to evaluate the clinical significance of USP10 in GC patients. USP10, E-cadherin, Ki67 and p53 expressions were detected in 365 GC and 40 non-cancerous mucosa tissues by immunohistochemistry. Western blot for USP10 was performed on additional fresh GC tissues and GC cell lines. The expression level of USP10 in GC tissues was proved lower than that in non-cancerous mucosa tissues (p < 0.05). It was also lower in GC cell lines (AGS, BGC-823 and MKN45 cells) than that in gastric epithelial immortalized cell line (GES-1). Clinicopathological analysis showed that USP10 expression was negatively correlated with gastric wall invasion (p = 0.009), nodal metastasis (p = 0.002), and TNM stage (p = 0.000). In contrast, a positively correlation between the expression of USP10 and E-cadherin was found (p < 0.05), but there was no relationship proved between Ki67, p53 and USP10 (p > 0.05). On the Kaplan–Meier survival curves, we found poor prognosis in GC patients was associated with negative USP10 expression (p < 0.05). Moreover, USP10 expression was an independent prognostic factor for the overall survival in multivariate analysis. Our findings suggested that USP10 was an independent predictor of prognosis of GC patients.

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Abbreviations

AR:

Androgen receptor

DUBs:

Deubiquitinases

G3BP:

Ras-GAP SH3 domain binding protein

GC:

Gastric carcinoma

IHC:

Immunohistochemistry

PHLPP:

PH domain leucine-rich-repeats protein phosphatase

SDS-PAGE:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

USPs:

Ubiquitin-specific proteases

USP10:

Ubiquitin-specific protease 10

USP18:

Ubiquitin-specific protease 18

USP2a:

Ubiquitin-specific protease 2a

USP22:

Ubiquitin-specific protease 22

USP46:

Ubiquitin-specific protease 46

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Acknowledgement

This work was supported in part by grants from Hubei Provincial Health Department Fund (Grant no. JX5B17). The authors are grateful to Mrs. Ying Chen and Mrs. Hong Hu for the excellent support in laboratory work.

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Authors and Affiliations

  1. Pathology Center, Shanghai First People`s Hospital / Faculty of Basic Medicine, Shanghai Jiao Tong University School of Medicine, Number 280, South Chong-Qing Road, Shanghai, 200025, China

    Zhi Zeng & Guo-Hui Fu

  2. Department of Pathology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China

    Zhi Zeng, Na Zhan, Ya-Bing Huang & Ze-Sheng Wang

  3. Department of Gastrointestinal Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China

    Hong-Xue Wu

  4. Department of Pathology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, Hubei, China

    Gui-Fang Yang

  5. Department of Pathology, Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, 200011, China

    Ping Wang

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  1. Zhi Zeng
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Correspondence to Zhi Zeng or Guo-Hui Fu.

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Z. Zeng and H.-X. Wu contributed equally to this work.

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Zeng, Z., Wu, HX., Zhan, N. et al. Prognostic significance of USP10 as a tumor-associated marker in gastric carcinoma. Tumor Biol. 35, 3845–3853 (2014). https://doi.org/10.1007/s13277-013-1509-1

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  • DOI: https://doi.org/10.1007/s13277-013-1509-1

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