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Clinical significance of RECK promoter methylation in pancreatic ductal adenocarcinoma

  • Research Article
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Tumor Biology

Abstract

The aim of this study was to analyze the clinical significance of reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) promoter methylation in pancreatic ductal adenocarcinoma (PDA). Methylation-specific polymerase chain reaction was used to examine the promoter methylation status of RECK in 60 pairs of PDA tissue samples and adjacent non-cancerous tissue samples. Statistical analyses were applied to test the associations between RECK promoter methylation status, clinicopathologic factors, and prognosis. The rate of RECK promoter methylation was significantly higher in PDA tissues than in adjacent non-cancerous tissues (P < 0.001). RECK methylation status was significantly associated with clinical stage (P = 0.017), histological differentiation (P = 0.046), and lymph node metastasis (P = 0.003), but was not associated with gender, age, and tumor location (all P > 0.05). Additionally, RECK promoter methylation is associated with malignant behavior and poor prognosis. In conclusion, determination of RECK promoter methylation status in tumor tissues may assist in the identification of patients who require aggressive postoperative intervention in order to improve prognosis.

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Authors and Affiliations

  1. Center of Organ Transplantation, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, No. 197, Ruijiner Road, Shanghai, 200025, China

    Xiong-Xiong Lu, Yuan Fang, Zheng-Ting Wang, Jun-Jie Xie, Qian Zhan, Xia-Xing Deng, Hao Chen, Jia-Bin Jin, Cheng-Hong Peng, Hong-Wei Li & Bai-Yong Shen

  2. Department of Radiation Oncology, Zhongshan Hospital, Fudan University, Shanghai, 200032, China

    Shu-Min Zhang

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  1. Xiong-Xiong Lu
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  2. Shu-Min Zhang
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  3. Yuan Fang
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  5. Jun-Jie Xie
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  7. Xia-Xing Deng
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Correspondence to Bai-Yong Shen.

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Xiong-Xiong Lu and Shu-Min Zhang contributed to this paper equally.

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Lu, XX., Zhang, SM., Fang, Y. et al. Clinical significance of RECK promoter methylation in pancreatic ductal adenocarcinoma. Tumor Biol. 34, 3339–3343 (2013). https://doi.org/10.1007/s13277-013-0903-z

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  • DOI: https://doi.org/10.1007/s13277-013-0903-z

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