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LncRNA LINC01123 promotes malignancy of ovarian cancer by targeting hsa-miR-516b-5p/VEGFA

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Abstract

Background

Long non-coding RNAs (lncRNAs) play a critical role in the development of ovarian cancer (OC).

Objective

The study aimed to determine the role of LncRNA LINC01123 in OC bio-progression, which is upregulated in OC tissues during OC progression.

Methods

Bioinformatics methods, GEPIA, and qRT-PCR were used to reveal the level and correlation of LINC01123, hsa-miR-516b-5p, and VEGFA, in OC cell lines. MTT, EdU, TUNEL, and Transwell assays were performed to assess the bioactivity of OC cell. Target sites of LINC01123 and hsa-miR-516b-5p were predicted using Starbase, and the potential linkage points of VEGFA and hsa-miR-516b-5p were predicted using TargetScan. These sites and linkage points were confirmed by double luciferase reporter assay.

Results

LINC01123 was upregulated in OC cell lines and LINC01123 silencing suppressed the proliferation and metastasis of OC cells, but promoted cell apoptosis. hsa-miR-516b-5p was linked to LINC01123 and. VEGFA was downstream of hsa-miR-516b-5p. Importantly, silencing of hsa-miR-516b-5p reversed the inhibitory impact of si-LINC01123. The result of hsa-miR-516b-5p inhibitor + si-LINC01123 co-transfection were rescued by si-VEGFA.

Conclusion

LINC01123 promotes OC development by dampening miR-516b-5p function, and may be a novel target for treating OC.

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Availability of data and material

The datasets used and/or analysed in the current study are available from the corresponding author upon reasonable request.

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Funding

This work was supported by the scientific research project of the basic scientific research business expenses of colleges and universities in Heilongjiang Province, 2020 (2020-KYYWF-0019).

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Authors and Affiliations

  1. Department of Gynaecology, The Third Affiliated Hospital of Qiqihar Medical University, No. 27, Taishun Street, Tiefeng, Qiqihar, 161000, Heilongjiang, China

    Bing Dong, Cuiping Li, Xiaomeng Xu, Yan Wang, Yuewen Li & Xingmei Li

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  1. Bing Dong
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  2. Cuiping Li
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  3. Xiaomeng Xu
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  4. Yan Wang
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  5. Yuewen Li
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  6. Xingmei Li
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Contributions

Research design: BD. Experimental operation: BD, CL, XL. Data statistics and analysis: YW, YL, XL. Draft writing: BD.

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Correspondence to Bing Dong.

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Dong, B., Li, C., Xu, X. et al. LncRNA LINC01123 promotes malignancy of ovarian cancer by targeting hsa-miR-516b-5p/VEGFA. Genes Genom 46, 231–239 (2024). https://doi.org/10.1007/s13258-023-01440-3

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