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Characterization of the B cell receptor repertoire of patients with acute coronary syndrome

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Abstract

Background

Acute coronary syndrome (ACS) is a complex cardiovascular disease whose development involves the dysregulation of adaptive immune responses. Though it has been proven that T cells associate with inflammation in the development of ACS, the function of B cells in disease remains unclear.

Objective

The aim of this study was to reveal the diversity of the B cell receptor (BCR) repertoire of patients with ACS.

Methods

We conducted a pilot study to sequence the immune repertoire of peripheral blood mononuclear cells (PBMCs) from patients with ACS, including acute myocardial infarction (AMI) and unstable angina (UA), and quantitatively characterized BCR repertoires by bioinformatics analysis.

Results

We found that patients with AMI and UA had lower BCR repertoire diversity compared with controls with normal coronary arteries (NCA). Lower percentages of productive unique BCR nt sequences and higher percentages of top 200 unique BCR sequences were identified in AMI and UA patients than NCA controls. Patients had various preferential usage of V and J genes from B cell clones in accordance with the disease severity of coronary arteries. AMI patients had distinct CDR3 amino acids, and their frequency differed among patients with ACS.

Conclusions

Our results indicate that differential BCR signatures represent an imprint of distinct repertoires among ACS patients. This study thereby opens up the prospect of studying disease-relevant B cells to better understand and treat ACS.

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Data availability

The datasets used and/or analyzed during the current study available from the corresponding author on reasonable request.

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Acknowledgements

The author would like to thank other colleagues whom were not listed in the authorship of Center for Cardiovascular Diseases and Center for Precision Medicine, Meizhou People’s Hospital (Huangtang Hospital), Meizhou Hospital Affiliated to Sun Yat-sen University for their helpful comments on the manuscript.

Funding

This study was supported by Key Scientific and Technological Project of Meizhou People’s Hospital, (Grant No.: MPHKSTP-20170101 and MPHKSTP-20180101 to MD Zhixiong Zhong), Science and Technology Program of Meizhou (Grant No.:2018B027 to Dr Sudong Liu), the Guangdong Provincial Key Laboratory of Precision Medicine and Clinical Translational Research of Hakka Population (Grant No.:2018B030322003).

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Authors and Affiliations

  1. Center for Precision Medicine, Meizhou People’s Hospital (Huangtang Hospital), Meizhou Hospital Affiliated to Sun Yat-sen University, No. 63 Huangtang Road, Meijiang District, Meizhou, 514031, People’s Republic of China

    Ruiqiang Weng, Sudong Liu, Xiaodong Gu & Zhixiong Zhong

  2. Guangdong Provincial Engineering and Technological Research Center for Molecular Diagnostics of Cardiovascular Diseases, Meizhou, 514031, People’s Republic of China

    Ruiqiang Weng, Sudong Liu, Xiaodong Gu & Zhixiong Zhong

  3. Guangdong Provincial Key Laboratory of Precision Medicine and Clinical Translational Research of Hakka Population, Meizhou, 514031, People’s Republic of China

    Ruiqiang Weng, Sudong Liu & Xiaodong Gu

  4. Center for Cardiovascular Diseases, Meizhou People’s Hospital (Huangtang Hospital), Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou, 514031, People’s Republic of China

    Zhixiong Zhong

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  1. Ruiqiang Weng
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  2. Sudong Liu
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  3. Xiaodong Gu
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  4. Zhixiong Zhong
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Contributions

RQW and ZXZ conceived and designed the experiments; recruited subjects and collected clinical data were performed by SDL and XDG. Data analysis and manuscript writing were performed by RQW and SDL. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Zhixiong Zhong.

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Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

This study was conducted in accordance with the declaration of Helsinki. This study was conducted with approval from the Ethics Committee of the Meizhou People’s Hospital (Huangtang Hospital), Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou 514031, P. R. China (No.: MPH-HEC 2016-A-43). Written informed consent was obtained from each patient..

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Weng, R., Liu, S., Gu, X. et al. Characterization of the B cell receptor repertoire of patients with acute coronary syndrome. Genes Genom 44, 19–28 (2022). https://doi.org/10.1007/s13258-021-01110-2

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  • DOI: https://doi.org/10.1007/s13258-021-01110-2

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