Skip to main content
SpringerLink
Log in

Association between expression levels and growth trait-related SNPs located in promoters of the MC4R and MSTN genes in Spinibarbus hollandi

  • Research Article
  • Published:
Genes & Genomics Aims and scope Submit manuscript

Abstract

Melanocortin 4 receptor: (MC4R) and Myostatin (MSTN) are two important growth trait-related genes in animals. In this study, we showed that two SNPs, MC4R-719A>G and MSTN-519C>T, found in the promoters of the MC4R and MSTN genes, respectively, are both associated with growth traits in Spinibarbus hollandi. Furthermore, we observed that there were significant associations between the expression levels of the MC4R and MSTN genes and these two growth trait-related SNPs. The expression level of MC4R gene in brain was lower in GG genotype fish with extremely high growth performance than that in AA genotype fish with extremely low growth performance. Expression level of the MSTN gene in muscle was lower in TT genotype fish with extremely high growth performance than that in CC and CT genotype fish with lower growth performance. The results indicated that these SNPs located in the promoters of MC4R and MSTN are associated with growth-related traits through modification of gene expression levels. The MSTN and MC4R SNPs may have useful application in effective marker-assisted selection aimed to increase output in S. hollandi.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Acosta J, Carpio Y, Borroto I, González O, Estrada MP (2005) Myostatin gene silenced by RNAi show a zebrafish giant phenotype. J Biotechnol 119:324–331

    Article  CAS  Google Scholar 

  • Amali AA, Lin CJF, Chen YH, Wang WL, Gong HY, Lee CY, Ko YL, Lu JK, Her GM, Chen TT (2004) Up-regulation of muscle-specific transcription factors during embryonic somitogenesis of zebrafish (Danio rerio) by knock-down of Myostatin-1. Dev Dynam 229:847–856

    Article  CAS  Google Scholar 

  • Cai ZD, Lin G, Ni JY, Ye Z, Tang ZB, Zhang S (2007) Study on the reproductive biology of Spinibabus calddwellis. Guangxi Agric Sci 2:24–28 (Chinese with English abstract)

    Google Scholar 

  • Cai X, Mipam TD, Zhao FF, Sun L (2015) SNPs detected in the yak MC4R gene and their association with growth traits. Animal 9:1097–1103

    Article  CAS  Google Scholar 

  • Carroll L, Voisey J, Van DA (2005) Gene polymorphisms and their effects in the melanocortin system. Peptides 26:1871–1885

    Article  CAS  Google Scholar 

  • Fan W, Boston BA, Kesterson RA, Hruby VJ, Cone RD (1997) Role of melanocortinergic neurons in feeding and the agouti obesity syndrome. Nature 385:165–168

    Article  CAS  Google Scholar 

  • Fontanesi L, Scotti E, Cisarova K, Di BP, Dall’Olio S, Fornasini D (2013) A missense mutation in the rabbit melanocortin 4 receptor (MC4R) gene is associated with finishing weight in a meat rabbit line. Anim Biotechnol 24:268–277

    Article  CAS  Google Scholar 

  • Guo L, Li L, Zhang S, Guo X, Zhang G (2011) Novel polymorphisms in the Myostatin, gene and their association with growth traits in a variety of bay scallop, Argopecten irradians. Anim Genet 42:339–340

    Article  CAS  Google Scholar 

  • Hickford JGH, Forrest RH, Zhou H, Fang Q, Han J, Frampton CM, Horrell AL (2010) Polymorphisms in the ovine Myostatin gene (MSTN) and their association with growth and carcass traits in New Zealand Romney sheep. Anim Genet 41:64–72

    Article  CAS  Google Scholar 

  • Houston DD, Elzinga DB, Maughan PJ, Smith SM, Kauwe JS, Evans RP (2012) Single nucleotide polymorphism discovery in cutthroat trout subspecies using genome reduction, barcoding, and 454 pyro-sequencing. BMC Genom 13:724

    Article  CAS  Google Scholar 

  • Huszar D, Lynch CA, Fairchildhuntress V, Dunmore JH, Fang Q, Berkemeier LR (1997) Targeted disruption of the melanocortin-4 receptor results in obesity in mice. Cell 88:131–141

    Article  CAS  Google Scholar 

  • Jiang YL, Li N, Fan XZ, Xiao LR, Xiang RL, Hu XX (2002) Associations of t leads to a mutation in the promoter region of Myostatin gene with birth weight in yorkshire pigs. Asian Austral J Anim 15:1543–1545

    Article  CAS  Google Scholar 

  • Kambadur R, Sharma M, Smith TP, Bass JJ (1997) Mutations in Myostatin (GDF8) in double-muscled belgian blue and piedmontese cattle. Genome Res 7:910–915

    Article  CAS  Google Scholar 

  • Kim KS, Larsen N, Short T, Plastow G, Rothschild MF (2000) A missense variant of the porcine melanocortin-4 receptor (MC4R) gene is associated with fatness, growth, and feed intake traits. Mamm Genome 11:131–135

    Article  CAS  Google Scholar 

  • Lee SJ, Mcpherron AC (1999) Myostatin and the control of skeletal muscle mass: commentary. Curr Opin Genet Dev 9:604–607

    Article  CAS  Google Scholar 

  • Lee CY, Hu SY, Gong HY, Chen MH, Lu JK, Wu JL (2009) Suppression of Myostatin with vector-based RNA interference causes a double-muscle effect in transgenic zebrafish. Biochem Bioph Res Commun 387:766–771

    Article  CAS  Google Scholar 

  • Lee Y, Park S, Kim H, Lee SK, Kim JW, Lee HK, Lee SJ (2013) A C1069G SNP of the MC4R gene and its association with economic traits in Korean native cattle (brown, brindle, and black). Electron J Biotechnol 16:14–14

    Article  Google Scholar 

  • Li W, Chen Q, Huang Y (2011) Laboratory study on ethology of Spinibarbus hollandi. Acta Ecol Sin 31:1404–1411

    Google Scholar 

  • Li H, Fan J, Liu S, Yang Q, Mu G, He C (2012) Characterization of a Myostatin gene (MSTN1) from spotted halibut (Verasper variegatus) and association between its promoter polymorphism and individual growth performance. Comp Biochem Phys B 161:315–322

    Article  CAS  Google Scholar 

  • Liu FP, Bai JJ, Xing YE, Li SJ, Li XH, Yu LY (2009) Cloning of MC4R gene and study on the association between SNPs of MC4R and growth trait in tilapia Oreochromis niloticus. J Fish Sci China 16:816–823 (Chinese with English abstract)

    CAS  Google Scholar 

  • Liu L, Yu X, Tong J (2012) Molecular characterization of Myostatin (MSTN) gene and association analysis with growth traits in the bighead carp (Aristichthys nobilis). Mol Biol Rep 39:9211–9221

    Article  CAS  Google Scholar 

  • Lv YJ, Zhang S, Lin G, Ni JY, Ye Z (2008) Research on the high-yielding Spininbabus hollandi oshima cultured in cage. J Anhui Agric Sci 36:15522–15523 (Chinese with English abstract)

    Google Scholar 

  • Mcpherron AC, Lee SJ (1997) Double muscling in cattle due to mutations in the Myostatin gene. Proc Natl Acad Sci USA 94:12457–12461

    Article  CAS  Google Scholar 

  • McPherron AC, Lawler AM, Lee SJ (1997) Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. Nature 387:83–90

    Article  CAS  Google Scholar 

  • Meidtner K, Wermter AK, Hinney A, Remschmidt H, Hebebrand J, Fries R (2010) Association of the melanocortin 4 receptor with feed intake and daily gain in F2 mangalitsa × pietrain pigs. Anim Genet 37:245–247

    Article  Google Scholar 

  • Meng X, Wang H, Qiu X, Liu H, Wang X (2017) SNPs of myostatin (MSTN) gene and their association with growth traits in three bay scallop (Argopecten irradians) populations. Aquac Res 48:531–536

    Article  CAS  Google Scholar 

  • Peñaloza C, Hamilton A, Guy DR, Bishop SC, Houston RD (2013) A SNP in the 5′ flanking region of the Myostatin-1b gene is associated with harvest traits in Atlantic salmon (Salmo salar). BMC Genom 14:112

    Article  Google Scholar 

  • Poćwierz-Kotus A, Bernaś R, Dębowski P, Kent MP, Lien S, Kesler M, Wenne R (2014) Genetic differentiation of southeast Baltic populations of sea trout inferred from single nucleotide polymorphisms. Anim Genet 45:96–104

    Article  Google Scholar 

  • Sánchez-Ramos I, Cross I, Mácha J, Martossa-Rodríguez G, Krylov V, Rebordinos L (2012) Assessment of tools for marker-assisted selection in a marine commercial species: Significant association between MSTN-1 gene polymorphism and growth traits. Sci World J 2012: 369802

  • Sellick GS, Pitchford WS, Morris CA, Cullen NG, Crawford AM, Raadsma HW (2007) Effect of Myostatin F94L on carcass yield in cattle. Anim Genet 38:440–446

    Article  CAS  Google Scholar 

  • Shu H, Yue L, Li Q, Lan ZJ, Gu PH, Wang HJ, Wu TJ (2015) Genetic variability of mtDNA COI genes in Spinibarbus hollandi. Oceanol Limnol Sin 46:1509–1515 (Chinese with English abstract)

    Google Scholar 

  • Song XM, Jiang JF, Zhang GZ, Shi FX, Jiang YQ (2012) DNA polymorphisms of the hu sheep melanocortin-4 receptor gene associated with birth weight and 45-day weaning weight. Genet Mol Res 11:4432–4441

    Article  CAS  Google Scholar 

  • Sun Y, Yu X, Tong J (2012) Polymorphisms in Myostatin gene and associations with growth traits in the common carp (Cyprinus carpio L.). Int J Mol Sci 13:14956–14961

    Article  CAS  Google Scholar 

  • Vaiman D, Mercier D, Moazami-Goudarzi K, Eggen A, Ciampolini R, Lépingle A (1994) A set of 99 cattle microsatellites: characterization, synteny mapping, and polymorphism. Mamm Genome 5:288–297

    Article  CAS  Google Scholar 

  • Wang FQ (2013) Morphological variation analysis of different Spinibarbus hollandi Groups. J Anhui Agric Sci 41:2464–2465 (Chinese with English abstract)

    Google Scholar 

  • Yang J, Liu Z, Shi H, Zhang J, Wang J, Huang J (2016) Association between MHC II beta chain gene polymorphisms and resistance to infectious haematopoietic necrosis virus in rainbow trout (Oncorhynchus mykiss, walbaum, 1792). Aquac Res 47:570–578

    Article  CAS  Google Scholar 

  • Zeng R, Zhang Y, Du P (2014) SNPs of melanocortin 4 receptor (MC4R) associated with body weight in beagle dogs. Exp Anim 63:73–78

    Article  CAS  Google Scholar 

  • Zhang CL, Chen H, Wang YH, Zhang L, Zhang AL, Zhang RF (2006) Association of a missense mutation of the MC4R gene with growth traits in cattle (brief report). Arch Tierz 49:515–516

    CAS  Google Scholar 

  • Zhang L, Qiu XM, Wang J, Jiang ZQ, Liu Y, Wang XL (2012) Polymorphism analysis on melanocortin-4 receptor (MC4R) gene in Takifugu rubripes. Biotechn Bull 33:97–102 (Chinese with English abstract)

    Google Scholar 

  • Zhu YY, Liang HW, Li Z, Luo XZ, Li L, Zhang ZW (2012) Polymorphism of MSTN gene and its association with growth traits in yellow catfish (Pelteobagruse fulvidraco). Hereditas 34:72–78

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work is supported by the National public industry major projects of China (No. 201303048) and the Guangdong Provincial Science and Technology Program (No. 2014A020208145).

Author information

Authors and Affiliations

  1. School of Life Science, Guangzhou University, Guangzhou, 510006, China

    Yang Yang, Hu Shu, Huiqiang Zhou, Xiaolu Jiang & Liping Hou

  2. Shaoguan Fisheries Research Institute, Shaoguan, 512000, China

    Zhaojun Lan & Pinghua Gu

Authors
  1. Yang Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhaojun Lan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hu Shu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Huiqiang Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiaolu Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Liping Hou
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Pinghua Gu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hu Shu.

Ethics declarations

Conflict of interest

Yang Yang declares that he does not have conflict of interest. Zhaojun LAN declares that he does not have conflict of interest. Hu Shu declares that he does not have conflict of interest. Huiqiang Zhou declares that he does not have conflict of interest. Xiaolu Jiang declares that she does not have conflict of interest. Liping Hou declares that she does not have conflict of interest. Pinghua Gu declares that he does not have conflict of interest.

Research involving human and animal rights

All animal experiments throughout the study were conducted in accordance with the Regulations for the Administration of Affairs Concerning Experimental Animals for the Science and Technology Bureau of China.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yang, Y., Lan, Z., Shu, H. et al. Association between expression levels and growth trait-related SNPs located in promoters of the MC4R and MSTN genes in Spinibarbus hollandi. Genes Genom 40, 1119–1125 (2018). https://doi.org/10.1007/s13258-018-0666-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13258-018-0666-4

Keywords

Search

Navigation