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Non-specific protein removal and specific protein capture simultaneously using a hydrodynamic force induced under vortex flow

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Abstract

Non-specific binding (NSB) of proteins should be removed to increase the sensitivity of biosensors. In this study, cadmium selenide nanoparticles (CdSe NPs) were rotated in the solution by generating vortex flow, thereby achieved removal of two types of non-specific protein: (i) anti-mouse IgG-FITC (NSB) and (ii) bovine serum albumin (BSA)-FITC. By manipulating rotation rate from 0 to 1000 rpm, photoluminescence (PL) from each protein reduced up to 28% for anti-mouse IgG and 35% for BSA, respectively. In addition, capturing specific proteins from mixture solutions enhanced by 41% upon cyclic-mode vortexing, which is 2.7 times higher than conventional continuous vortexing. These results can open up a new feasibility for increasing sensitivity of biosensors just by utilizing properly the hydrodynamic force.

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Acknowledgements

This work was supported by National Research Foundation of Korea (NRF-2021R1A2C3009955), and a Korea University Grant.

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Authors and Affiliations

  1. Department of Chemical and Biological Engineering, Korea University, Seoul, 02841, Korea

    Hanzhe Liu & Dong June Ahn

  2. KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Korea

    Dong June Ahn

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  1. Hanzhe Liu
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  2. Dong June Ahn
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DJA designed and supervised the project. HL carried out the experiment and analyzed the data.

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Correspondence to Dong June Ahn.

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Liu, H., Ahn, D.J. Non-specific protein removal and specific protein capture simultaneously using a hydrodynamic force induced under vortex flow. Macromol. Res. 31, 85–90 (2023). https://doi.org/10.1007/s13233-023-00131-y

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  • DOI: https://doi.org/10.1007/s13233-023-00131-y

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