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Precipitation-Mediated PEGylation of Plant-Derived Nanovesicles

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Abstract

Recently, plant-derived nanovesicles have attracted interest in the field of regenerative medicine because of their abundant immune-regulatory RNAs and phytochemicals with cell proliferation activity. However, the poor stability and rapid clearance of vesicles remain major challenges in their clinical applications. Herein, we report a facile method for surface modification of plant-derived nanovesicles by precipitation in the presence of poly(ethylene glycol) (PEG). We confirmed that PEGylation of nanovesicles slightly increased the particle size and zeta potential values. Importantly, this simple precipitation method produced PEGylated nanovesicles without any quantitative or qualitative loss of internal contents. When dispersed in PBS (pH 7.4), PEGylated nanovesicles did not exhibit a significant change in size for 24 h, indicating their high stability. Overall, our precipitation-based method is a useful technique for PEGylation of plant-derived nanovesicles without loss of bioactivity.

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Author notes

  1. These authors contributed equally to this study.

Authors and Affiliations

  1. School of Chemical Engineering, College of Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, Gyeonggi, 16419, Korea

    Van Quy Nguyen, Wooram Um, Jae Yoon An, Hyeyeon Joo, Jae Min Jung, Dong Gil You & Jae Hyung Park

  2. ExoStemTech Inc., Ansan, Gyeonggi, 15588, Korea

    Young Chan Choi, Ji Suk Choi, Yong Woo Cho & Jae Hyung Park

  3. Department of Materials Science and Chemical Engineering, Hanyang University ERICA, Ansan, Gyeonggi, 15588, Korea

    Yong Woo Cho

  4. Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, Gyeonggi, 16419, Korea

    Jae Hyung Park

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  1. Van Quy Nguyen
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  2. Wooram Um
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  7. Ji Suk Choi
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  8. Dong Gil You
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  9. Yong Woo Cho
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Contributions

Van quy nguyen: Conceptualization, Investigation, Formal analysis, Data curation, Writing — original draft, Visualization. Wooram um: Investigation, Formal analysis, Data curation, Writing — original draft, Visualization. Jae Yoon An: Investigation, Data curation, Writing — review & editing. Hyeyeon Joo: Investigation, Data curation, Visualization. Yong Chan Choi: Conceptualization. Jaemin Jung: Data curation. Ji Suk Choi: Visualization. Yong Woo Cho: Resources, Data curation, Visualization. Jae Hyung Park: Resources, Supervision, Visualization, Project administration, Writing — review & editing.

Corresponding authors

Correspondence to Yong Woo Cho or Jae Hyung Park.

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Acknowledgment: This work was supported by Korea Health Technology R&D Project (HI20C0437) of the Ministry of Health & Welfare, Basic Science Research Program (2020R1A6A3A01100335) through the National Research Foundation of Korea (NRF), Korea Basic Science Institute (National research Facilities and Equipment Center) grant funded by the Ministry of Education (2020R1A6C101A191), and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2021R1A4A1032782), Republic of Korea.

Footnote: The image from this article is used as the cover image of the Volume 30, Issue 2.

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Nguyen, V.Q., Um, W., An, J.Y. et al. Precipitation-Mediated PEGylation of Plant-Derived Nanovesicles. Macromol. Res. 30, 85–89 (2022). https://doi.org/10.1007/s13233-022-0016-x

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