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Cellular response of silk fibroin nanofibers containing silver nanoparticles In vitro

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Abstract

Silk Fibroin (SF) nanofibers containing various silver compounds, such as silver tetrafluoroborate, silver phosphate, silver sulfadiazine, and silver acetate, were prepared by electrospinning. Silver compounds were dissolved into SF solution in 98% formic acid. In this system, the formic acid was used both as a co-solvent of SF and silver compounds, and as a reducing agent for silver ions in solution. The average diameters of SF nanofibers varied depending on the type of silver compounds added. The Ag element in the SF nanofibers was confirmed to be mainly Ag nanoparticles by XPS results. The in vitro cytotoxicity of silver compounds in SF nanofibers on normal human keratinocytes and fibroblasts was examined. Overall, the silver compounds in SF nanofibers are cytotoxic to both normal human epidermal keratinocytes (NHEK) and normal human epidermal fibroblasts (NHEF) cells.

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Authors and Affiliations

  1. Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon, 305-764, Korea

    Lim Jeong & Won Ho Park

  2. Department of Polymer Science and Engineering, Kumoh Institute of Technology, Gyeongbuk, 730-701, Korea

    Donghwan Cho & Oh Hyeong Kwon

  3. Department of Oral Biochemistry, Dental Research Institute, Seoul National University, School of Dentistry, Seoul, 110-744, Korea

    Byung-Moo Min

Authors
  1. Lim Jeong
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  2. Donghwan Cho
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  3. Oh Hyeong Kwon
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  4. Byung-Moo Min
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  5. Won Ho Park
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Correspondence to Byung-Moo Min or Won Ho Park.

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Jeong, L., Cho, D., Kwon, O.H. et al. Cellular response of silk fibroin nanofibers containing silver nanoparticles In vitro . Macromol. Res. 22, 796–803 (2014). https://doi.org/10.1007/s13233-014-2104-z

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  • DOI: https://doi.org/10.1007/s13233-014-2104-z

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