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Fabrication of hydrogel scaffolds using rapid prototyping for soft tissue engineering

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Abstract

Three-dimensional (3-D) scaffolds require an interconnected structure with controllable and reproducible porosity to guide cell growth and tissue regeneration for tissue engineering and regenerative medicine. In this study, a cell-plotting system was developed using a solid freeform fabrication (SFF) technique and fabricated hydrogel scaffolds with an interconnected pore structure using various hydrogels such as agarose, Pluronic® F127, and alginate. The plotting process was characterized using discharge data and the thicknesses of the hydrogel lines. Alginate was plotted under a range of pressures, with optimal plotting at 120 and 220 kPa for 2% and 4% alginate, respectively. The 4% alginate hydrogel was more stable in the plotting process than the 2% alginate. Cells seeded in the hydrogel showed good cell viability in a preliminary in vitro test. 3-D hydrogel scaffolds were produced successfully for soft tissue engineering applications.

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Authors and Affiliations

  1. Nature-Inspired Mechanical System Team, Nano Convergence & Manufacturing Systems Research Division, Korea Institute of Machinery & Materials (KIMM), Daejeon, 305-343, Korea

    Su A. Park, Su Hee Lee & WanDoo Kim

Authors
  1. Su A. Park
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  2. Su Hee Lee
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  3. WanDoo Kim
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Correspondence to Su A. Park.

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Park, S.A., Lee, S.H. & Kim, W. Fabrication of hydrogel scaffolds using rapid prototyping for soft tissue engineering. Macromol. Res. 19, 694–698 (2011). https://doi.org/10.1007/s13233-011-0708-0

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  • DOI: https://doi.org/10.1007/s13233-011-0708-0

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