Abstract
An effective biosurfactant-producing strain, denoted ZG0427, was isolated from oil-contaminated soil samples collected from an oilfield in northwest China using the drop-collapse test, oil displacement test, and surface tension measurements. The strain was identified as Bacillus sp. based on the results of morphological and biochemical tests and 16S rDNA sequence analysis. The kinetics of cell growth and biosurfactant production were monitored. A maximum biosurfactant production of 2.79 g/L was achieved when the cells were cultivated in fermentation medium containing 2 % (w/v) of glucose as the sole carbon source, with shaking (180 rpm) at 37 °C for 32 h. The extracted biosurfactant reduced the surface tension of deionized water to 24.6 mN/m, with a critical micelle concentration of 50 mg/L, and showed a remarkable ability to reduce both surface tension and emulsification over wide ranges of pH (5.0–12.0), temperature (−70 to 121 °C), and NaCl concentrations (0–10 %, w/v), respectively. The biosurfactant was extracted from the culture supernatant and partially characterized as lipopeptide.
Similar content being viewed by others
References
Abdel-Mawgoud AM, Aboulwafa MM, Hassouna NAH (2009) Characterization of rhamnolipid produced by Pseudomonas aeruginosa isolate Bs20. Appl Biochem Biotechnol 157:329–345
Amiriyan A, Assadi MM, Saggadian VA, Noohi A (2004) Bioemulsan production by Iranian oil reservoirs microorganisms. Iran J Environ Health Sci Eng 1:28–35
Aparna A, Srinikethan G, Smitha H (2012) Production and characterization of biosurfactant produced by a novel Pseudomonas sp. 2B. Colloids Surf B Biointerfaces 95:23–29
Arguelles-Arias A, Ongena M, Halimi B, Lara Y, Brans A, Joris B, Fickers P (2009) Bacillus amyloliquefaciens GA1 as a source of potent antibiotics and other secondary metabolites for biocontrol of plant pathogens. Microbial Cell Fact 8:63
Banat IM, Makkar RS, Cameotra SS (2000) Potential commercial applications of microbial surfactants. Appl Biochem Biotechnol 53:495–508
Banat IM, Franzetti A, Gandolfi I, Bestetti G, Martinotti MG, Fracchia L, Smyth TJ, Marchant R (2010) Microbial biosurfactants production, applications and future potential. Appl Microbiol Biotechnol 87:427–444
Batista SB, Mounteer AH, Amorim FR, Tótola MR (2006) Isolation and characterization of biosurfactant/bioemulsifier-producing bacteria from petroleum contaminated sites. Bioresour Technol 97:868–875
Bicca FC, Fleck LC, Ayub MAZ (1999) Production of biosurfactant by hydrocarbon degrading Rhodococcus ruber and Rhodococcus erythropolis. Rev Microbiol 30:231–236
Bodour AA, Miller-Maier RM (1998) Application of a modified drop-collapse technique for surfactant quantitation and screening of biosurfactant-producing microorganisms. J Microbiol Methods 32:273–280
Chandankere R, Yao J, Choi MMF, Masakorala K, Chan Y (2013) An efficient biosurfactant-producing and crude-oil emulsifying bacterium Bacillus methylotrophicus USTBa isolated from petroleum reservoir. Biochem Eng J 74:46–53
Cheng F, Cheng T, Huan Y, Yu HM, Yu C, Shen ZY (2013) Characterization of a blend-biosurfactant of glycolipid and lipopeptide produced by Bacillus subtilis TU2 isolated from underground oil-extraction wastewater. J Microbiol Biotechnol 23:390–396
Cooper DG, Zajic JE, Gerson DF (1979) Production of surface active lipids by Corynebacterium lepus. Appl Environ Microbiol 37:4–10
Das K, Mukherjee AK (2005) Characterization of biochemical properties and biological activities of biosurfactants produced by Pseudomonas aeruginosa mucoid and non-mucoid strains isolated from hydrocarbon-contaminated soil samples. Appl Microbiol Biotechnol 69:192–199
Desai JD, Banat IM (1997) Microbial production of surfactants and their commercial potential. Microbiol Mol Biol Rev 61:47–64
Felse PA, Shah V, Chan J, Rao KJ, Gross RA (2007) Sophorolipid biosynthesis by Candida bombicola from industrial fatty acid residues. Enzyme Microb Technol 40:316–323
Geetha I, Manonmani AM (2010) Surfactin: a novel mosquitocidal biosurfactant produced by Bacillus subtilis ssp. subtilis (VCRC B471) and influence of abiotic factors on its pupicidal efficacy. Lett Appl Microbiol 51:406–412
Ghojavand H, Vahabzadeh F, Mehranian M, Radmehr M, Shahraki KA, Zolfagharian F, Emadi MA, Roayaei E (2008) Isolation of thermotolerant, halotolerant, facultative biosurfactant-producing bacteria. Appl Microbiol Biotechnol 80:1073–1085
Ibrahim ML, Ijah UJJ, Manga SB, Bilbis LS, Umar S (2013) Production and partial characterization of biosurfactant produced by crude oil degrading bacteria. Int Biodeter Biodegr 81:28–34
Jadhav M, Kalme S, Tamboli D, Govindwar S (2011) Rhamnolipid from Pseudomonas desmolyticum NCIM-2112 and its role in the degradation of brown 3REL. J Basic Microbiol 51:385–396
Khopade A, Biao R, Liu X, Mahadik K, Zhang L, Kokare C (2012) Production and stability studies of the biosurfactant isolated from marine Nocardiopsis sp. B4. Desalination 285:198–204
Kosaric N (2001) Biosurfactants and their application for soil bioremediation. Food Technol Biotechnol 39:295–304
Krishnaswamy M, Subbuchettiar G, Ravi TK, Panchaksharam S (2008) Biosurfactants: properties, commercial production and application. Curr Sci 94:736–774
Kumar AS, Mody K, Jha B (2007) Evaluation of biosurfactant/bioemulsifier production by a marine bacterium. Bull Environ Contam Toxicol 79:617–621
Maneerat S, Phetrong K (2007) Isolation of biosurfactant-producing marine bacteria and characteristics of selected biosurfactan. Songklanakarin J Sci Technol 29:781–791
Noparat P, Maneerat S, Saimmai A (2014) Application of biosurfactant from Sphingobacterium spiritivorum AS43 in the biodegradation of used lubricating oil. Appl Biochem Biotechnol 172:3949–3963
Pacheco GJ, Ciapina EMP, Gomes EB, Junior NP (2010) Biosurfactant production by Rhodococcus erythropolis and its application to oil removal. Braz J Microbiol 41:685–693
Pacwa-Plociniczak M, Plaza GA, Piotrowska-Seget Z, Cameotra SS (2011) Environmental applications of biosurfactants: recent advances. Int J Mol Sci 12:633–654
Padmapriya B, Rajeswari T, Suganthi S, Rajeswari T, Jayalakshmi S (2011) Biosurfactant production and plasmid isolation from newly isolated hydrocarbonoclastic bacteria Proteus inconstans. Int J Pharma Biol Arch 2:784–790
Perfumo A, Smyth TJP, Marchant R, Banat IM (2010) Production and roles of biosurfactants and bioemulsifiers in accessing hydrophobic substrates. In: Timmis KN (ed) Handbook of hydrocarbon and lipid microbiology. Springer, Berlin Heidelberg, pp 1502–1510
Peypoux F, Michel G (1992) Controlled biosynthesis of Val7- and Leu7- surfactins. Appl Microbiol Biotechnol 36:515–517
Pornsunthorntawee O, Arttaweeporn N, Paisanjit S, Somboonthanate P, Abe M, Rujiravanit R, Chavadej S (2008) Isolation and comparison of biosurfactants produced by Bacillus subtilis PT2 and Pseudomonas aeruginosa SP4 for microbial surfactant-enhanced oil recovery. Biochem Eng J 42:172–179
Prats C, Giró A, Ferrer J, López D, Vives-Rego J (2008) Analysis and lbM simulation of the stages in bacterial lag phase: Basis for an updated definition. J Theor Biol 252:56–68
Rahman PKSM, Gakpe E (2008) Production, Characterisation and applications of biosurfactants—review. Biotechnology 7:360–370
Roongsawang N, Thaniyavarn J, Thaniyavarn S, Kameyama T, Haruki M, Imanaka T, Morikawa M, Kanaya S (2002) Isolation and characterization of a halotolerant Bacillus subtilis BBK-1 which produces three kinds of lipopeptides: bacillomycin L, plipastatin, and surfactin. Extremophiles 6:499–506
Safary A, Ardakani MR, Suraki AA, Khiavi MA, Motamedi H (2010) Isolation and characterization of biosurfactant producing bacteria from Caspian sea. Biotechnology 9:378–382
Saimmai A, Rukadee O, Onlamool T, Sobhon V, Maneerat S (2012) Isolation and functional characterization of a biosurfactant produced by a new and promising strain of Oleomonas sagaranensis AT18. World J Microbiol Biotechnol 28:2973–2986
Sang-Cheol KSH, Park IH, Chung SY, Chandra MS, Choi YL (2010) Isolation, purification, and characterization of novel fengycin S from Bacillus amyloliquefaciens LSC04 degrading-crude oil. Biotechnol Bioproc Eng 15:246–253
Saravanan V, Vijayakumar S (2012) Isolation and screening of biosurfactant producing microorganisms from oil contaminated soil. J Acad Ind Res 1:264–268
Schaller KD, Fox SL, Bruhn DF, Noah KS, Bala GA (2004) Characterization of surfactin from Bacillus subtilis for application as an agent for enhanced oil recovery. Appl Biochem Biotechnol 115:827–836
Shavandi M, Mohebali G, Haddadi A, Shakarami H, Nuhi A (2011) Emulsification potential of a newly isolated biosurfactant-producing bacterium, Rhodococcus sp. strain TA6. Colloids Surf B Biointerfaces 83:477–482
Singh A, Hamme JDV, Ward OP (2006) Surfactants in microbiology and biotechnology: part 2. Application aspects. Biotechnol Adv 25:99–121
Smyth TJP, Perfumo A, McClean S, Marchant R, Banat IM (2010) Isolation and analysis of lipopeptides and high molecular weight biosurfactants. In: Timmis KN (ed) Handbook of hydrocarbon and lipid microbiology. Springer, Berlin Heidelberg, pp 3689–3703
Sneha KS, Padmapriya B, Rajeswari T (2012) Isolation and screening of biosurfactants produced by Pseudomonas aeruginosa from oil spilled soils. Int J Pharm Biol Arch 3:321–325
Tabatabaee A, Assadi MM, Noohi AA, Sajadian VA (2005) Isolation of biosurfactant producing bacteria from oil reservoirs. Iran J Environ Health Sci Eng 2:6–12
Techaoei S, Lumyong S, Prathumpai W, Santiarwarn D, Leelapornpisid P (2011) Screening characterization and stability of biosurfactant produced by Pseudomonas aeruginosa SCMU106 isolated from soil in northern Thailand. Asian J Biol Sci 4:340–351
Tugrul T, Cansunar E (2005) Detecting surfactant-producing microorganisms by the drop-collapse test. World J Microbiol Biotechnol 21:851–853
Varadavenkatesan T, Murty VR (2013) Production and properties of a lipopeptide biosurfactant by B. subtilis subsp. inaquosorum. J Microbiol Biotechnol Res 3:63–73
Wei YH, Chou CL, Chang JS (2005) Rhamnolipid production by indigenous Pseudomonas aeruginosa J4 originating from petrochemical wastewater. Biochem Eng J 27:146–154
Whang LM, Liu PWG, Ma CC, Cheng SS (2008) Application of biosurfactants, rhamnolipid, and surfactin, for enhanced biodegradation of diesel-contaminated water and soil. J Hazard Mater 151:155–163
Willumsen PA, Karlson U (1997) Screening of bacteria, isolated from PAH-contaminated soils, for production of biosurfactants and bioemulsifiers. Biodegradation 7:415–423
Xia WJ, Luo Z, Dong HP, Li Y, Cui QF, Bi YQ (2012) Synthesis, characterization, and oil recovery application of biosurfactant produced by indigenous Pseudomonas aeruginosa WJ-1 using waste vegetable oils. Appl Biochem Biotechnol 166:1148–1166
Youssef NH, Duncan KE, Nagle DP, Savage KN, Knapp RM, McInerney MJ (2004) Comparison of methods to detect biosurfactant production by diverse microorganisms. J Microbiol Methods 56:339–347
Acknowledgments
This work was supported by grants of the National Natural Science Foundation of China (31272694).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhou, H., Chen, J., Yang, Z. et al. Biosurfactant production and characterization of Bacillus sp. ZG0427 isolated from oil-contaminated soil. Ann Microbiol 65, 2255–2264 (2015). https://doi.org/10.1007/s13213-015-1066-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13213-015-1066-5