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Enhanced cytidine production by a recombinant Escherichia coli strain using genetic manipulation strategies

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Abstract

Cytidine is a nucleoside molecule that is widely used as a precursor for antiviral drugs. In this study, a cytidine-producing strain Cyt18 was developed from Escherichia coli K-12 through 3-step genetic manipulation strategies. Cytidine deaminase gene (cdd) was firstly deleted from the E. coli K-12 strain to develop Cyt10. Furthermore, homoserine dehydrogenase gene (thrA) was inactivated from the Cyt10 strain to develop Cyt12, in which the intracellular aspartate concentration was expected to be increased. The recombinant plasmid pMG1105 containing an pyrB-pyrA operon from Bacillus amyloliquefaciens CYTI was constructed and was introduced into Cyt12 to obtain the Cyt18 strain. Compared to the Cyt12 strain, the cytidine production by the recombinant strain Cyt18 was increased by ~3-fold (722.9 mg/l vs. 249.3 mg/l).

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Acknowledgments

We thank our colleagues for critical reading of the manuscript and providing valuable suggestions. This work was supported by the Colleges Research Projects of Ningxia (2012), the Research Program of Tianjin University of Science and Technology (20100211) and by Program for Changjiang Scholars and Innovative Research Team in University (IRT 1166).

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Authors and Affiliations

  1. College of Agriculture, Ningxia University, Yinchuan, China

    Haitian Fang

  2. College of biotechnology, Tianjin University of Science and Technology, Tianjin, China

    Haitian Fang, Chenglin Zhang, Xixian Xie, Qingyang Xu, Yunjiao Zhou & Ning Chen

  3. Metabolic Engineering Laboratory, College of Biotechnology, Tianjin University of Science & Technology, No. 29, 13 Main Street, Tianjin Economic and Technological Development Zone, Tianjin, 300457, China

    Ning Chen

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  1. Haitian Fang
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  2. Chenglin Zhang
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Correspondence to Ning Chen.

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Fang, H., Zhang, C., Xie, X. et al. Enhanced cytidine production by a recombinant Escherichia coli strain using genetic manipulation strategies. Ann Microbiol 64, 1203–1210 (2014). https://doi.org/10.1007/s13213-013-0760-4

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  • DOI: https://doi.org/10.1007/s13213-013-0760-4

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