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Characterization of bacteria associated with nodules of two endemic legumes of Algeria, Hedysarum naudinianum and H. perrauderianum

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Abstract

The root nodules of two wild legume species endemic to Algeria, Hedysarum naudinianum and He. perrauderianum, were investigated with regard to their anatomy and histology, and the identity of the associated bacteria. Both plants were found to form root nodules with regular features and well infected by rod-shaped bacteria. The culturable fraction of bacteria that could be obtained from surface-sterilized nodules included a prevailing presence of Enterobacteriaceae having 100 % 16S rDNA sequence identity with both Enterobacter cloacae and E. ludwigii. In H. perrauderianum, this taxon was the sole cultured isolate, while from H. naudinianum we also found Bacillus, Lactobacillus, Staphylococcus, Rothia, and isolates that were 100 % identical to Corynebacterium pseudodiphthericum, which is known to be an agent of respiratory and cardiac infections in humans. Whereas no culturable rhizobia and alike could be obtained on plates, PCR-based culture-independent approaches revealed in both plants the presence of a Mesorhizobium sp., which in H. perrauderianum was identical to isolates nodulating other legumes from Africa, European Mediterranean countries, and Asia, while in H. naudinianum it bore a single nucleotide polymorphism which is so far unique for any observed mesorhizobia. Data from the microsymbionts appear to suggest interesting clues to interpret the evolutionary ecology of their host plants.

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Authors and Affiliations

  1. Département de Microbiologie, Faculté des Sciences de la Nature et de la Vie, Laboratoire de Biologie Moléculaire et Cellulaire, Université Constantine 1, Route de Aïn El Bey, Constantine, Algeria

    Asma Torche, Amar Benguedouar & Yacine Benhizia

  2. Department of Biology, University of Padova, Via Ugo Bassi 58/b, 35131, Padova, Italy

    Riccardo Rosselli, Ottavia Romoli, Enrico Baldan, Alessandra Tondello & Barbara Baldan

  3. Department of Agronomy Animals, Food, Natural Resources and Environment, DAFNAE, University of Padova, Viale dell’Università 16, 35020, Legnaro, Padova, Italy

    Marina Zanardo, Sara Alberghini & Andrea Squartini

  4. Département de Biologie Végétale, Faculté des Sciences de la Nature et de la Vie, Laboratoire de Génétique, Biochimie et Biotechnologies Végétales, Université Constantine 1, Route de Aïn El Bey, Constantine, Algeria

    Hayet Benhizia

Authors
  1. Asma Torche
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  2. Hayet Benhizia
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  3. Riccardo Rosselli
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  4. Ottavia Romoli
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  5. Marina Zanardo
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  6. Enrico Baldan
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  7. Sara Alberghini
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  8. Alessandra Tondello
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  9. Barbara Baldan
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  10. Amar Benguedouar
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  11. Andrea Squartini
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  12. Yacine Benhizia
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Corresponding author

Correspondence to Andrea Squartini.

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Torche, A., Benhizia, H., Rosselli, R. et al. Characterization of bacteria associated with nodules of two endemic legumes of Algeria, Hedysarum naudinianum and H. perrauderianum . Ann Microbiol 64, 1065–1071 (2014). https://doi.org/10.1007/s13213-013-0745-3

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  • DOI: https://doi.org/10.1007/s13213-013-0745-3

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