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Rock phosphate solubilization by four yeast strains

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Abstract

The solubilization of rock phosphate (RP) by four yeast strains, Rhodotorula sp., Candida rugosa, Saccharomyces cerevisiae and Saccharomyces rouxii, which were isolated from wheat rhizospheric soils, was investigated in this study. The yeast isolates demonstrated diverse levels of soluble phosphate releasing abilities in modified Pikovskaya liquid medium containing RP as sole phosphate source. C. rugosa was the most effective solubilizer under different conditions, followed by Rhodotorula sp., S. rouxii and S. cerevisiae. Acidification of the broth seemed to be the major mechanism for RP solubilization by the yeast isolates, and the increase in soluble phosphate released was correlated significantly with an increase in titratable acidity and a drop in pH. The optimal composition for the solubilization of RP by the yeast isolates in the broth was 20 g L−1 glucose, 1 g L−1 yeast extract, 0.5 g L−1 (NH4)2SO4, and 5 g L−1 RP, respectively. The yeast isolates were able to solubilize RP at wide range of temperature and initial pH, with the maximum percentage of soluble phosphate released being recorded at 30–35 °C and pH 5–6, respectively.

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Acknowledgments

This research work was kindly supported by National Natural Science Foundation of China (No. 51004078), Program for New Century Excellent Talents in University (NCET-11-0965), Program for Changjiang Scholars and Innovative Research Team in University (No. IRT0974), National Basic Research Program of China (No. 2011CB411901) and the Open Foundation of Key Laboratory for Green Chemical Process of Ministry of Education (GCP201104).

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  1. Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Lab of Novel Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan, 430073, People’s Republic of China

    Chunqiao Xiao, Ruan Chi, Xiao Pan, Feng Liu & Jiawei He

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  1. Chunqiao Xiao
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  2. Ruan Chi
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  3. Xiao Pan
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  4. Feng Liu
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  5. Jiawei He
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Correspondence to Ruan Chi.

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Xiao, C., Chi, R., Pan, X. et al. Rock phosphate solubilization by four yeast strains. Ann Microbiol 63, 173–178 (2013). https://doi.org/10.1007/s13213-012-0458-z

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  • DOI: https://doi.org/10.1007/s13213-012-0458-z

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