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Bacillus subtilis fadB (ysiB) gene encodes an enoyl-CoA hydratase

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Abstract

Fatty acids are essential components of membranes and are an important source of metabolic energy. In bacteria, the β-oxidation pathway is well known in Escherichia coli. Bacillus subtilis possesses a considerable number of genes, organized in five operons, that are most likely involved in the β-oxidation of fatty acids. Among these genes, only one product, FadRBs (YsiA), has been recently characterized as a transcriptional regulatory protein which negatively regulates the expression of β-oxidation genes including those belonging to the lcfA operon, including fadR Bs (ysiA). The probable involvement of the FadRBs (YsiA) regulon members in β-oxidation is inferred from data based on BLASTP similarity of their gene products. In this work, we report the cloning and the expression of B. subtilis fadB Bs(ysiB), belonging to the lcfA operon, and the functional characterization of its product as an enoyl-CoA hydratase, demonstrating the actual involvement of these genes in fatty acid β-oxidation.

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Author notes

  1. Massimiliano Marvasi

    Present address: Biology Department, Biotechnology Center, Pontifical Catholic University of Puerto Rico, 2250 Las Americas Avenue, Suite 504, Ponce, 00717-9997, PR, USA

Authors and Affiliations

  1. Dipartimento di Biologia Evoluzionistica “Leo Pardi”, Università di Firenze, Via Romana 17-19, 50125, Firenze, Italy

    Antonio Frandi, Massimiliano Marvasi, Giorgio Mastromei & Brunella Perito

  2. Dipartimento di Scienze e Tecnologie Biomediche (DiSTeB), Università di Cagliari, Complesso Universitario, 09042, Monserrato, (CA), Italy

    Paolo Zucca & Enrico Sanjust

  3. Department of Evolutionary Biology “Leo Pardi”, University of Florence, Via Romana 17-19, 50125, Florence, Italy

    Brunella Perito

Authors
  1. Antonio Frandi
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  2. Paolo Zucca
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  3. Massimiliano Marvasi
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  4. Giorgio Mastromei
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  5. Enrico Sanjust
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  6. Brunella Perito
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Corresponding author

Correspondence to Brunella Perito.

Additional information

Antonio Frandi, Paolo Zucca and Massimiliano Marvasi contributed equally to the work

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Frandi, A., Zucca, P., Marvasi, M. et al. Bacillus subtilis fadB (ysiB) gene encodes an enoyl-CoA hydratase. Ann Microbiol 61, 371–374 (2011). https://doi.org/10.1007/s13213-010-0121-5

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  • DOI: https://doi.org/10.1007/s13213-010-0121-5

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