Abstract
The passivation layers of an in situ-synthesized cadmium sulfide (CdS) nanowire (NW) photosensor were prepared for two reasons: (1) to improve the physical stability of the NW on an interdigitated electrode and (2) to enhance the immobilization efficiency of proteins for the on-chip immunoassay. The passivation layer was estimated to have suitable optical properties, and the photoresponse of photosensor was increased after the formation of passivation layer. The immobilization efficiency of a parylene-H film through covalent bonding was compared with the immobilization of Z-domains through physical adsorption. Finally, on-chip chemiluminescent immunoassay of bacteria was carried out by immobilizing antibodies against Escherichia coli through Z-domains for the orientation control of antibodies. The limit of detection was determined to be less than 104E. coli/mL (n=3), and the sensitivity of bacterial detection was estimated to be 0.339 pA/E. coli/mL (n=3) with a linearity factor (R2) of 0.999. These results showed that the on-chip chemiluminescent immunoassay for bacterial detection could be performed using passivation layer coated CdS NW photosensor.
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References
Blank, T. & Gol’dberg, Y.A. Semiconductor photoelectric converters for the ultraviolet region of the spectrum. Semiconductors 37, 999–1030 (2003).
Gao, T., Li, Q. & Wang, T. CdS nanobelts as photo-conductors. Appl. Phys. Lett. 86, 173105 (2005).
Hoang, T.B., Titova, L., Jackson, H., Smith, L., Yarrison-Rice, J., Lensch, J. & Lauhon, L.J. Temperature dependent photoluminescence of single CdS nanowires. Appl. Phys. Lett. 89, 123123 (2006).
Jie, J., Zhang, W., Jiang, Y., Meng, X., Li, Y. & Lee, S. Photoconductive characteristics of single-crystal CdS nanoribbons. Nano Lett. 6, 1887–1892 (2006).
Kim, H.-R., An, B.-G., Chang, Y.W., Kang, M.-J., Park, J.-G., Pyun, J.-C., Kim, H.-R., An, B.-G., Chang, Y.W. & Kang, M.-J. Characterization of in-situ synthesized CdS x Se 1 — x ternary alloy nanowire photosensor. J. Korean Ceram. Soc. 56, 308–316 (2019).
Kwon, G.-H., Kim, T.W., Lee, H.I., Cho, W.C., Kim, H., Kwon, G.-H., Kim, T.W., Lee, H.I., Cho, W.C. & Kim, H. Synthesis of ZrO2 nanorods and their application as membrane materials. J. Korean Ceram. Soc. 56, 541–548 (2019).
Li, Q., Gao, T. & Wang, T. Optoelectronic characteristics of single CdS nanobelts. Appl. Phys. Lett. 86, 193109 (2005).
Ma, R., Wei, X., Dai, L., Huo, H. & Qin, G. Synthesis of CdS nanowire networks and their optical and electrical properties. Nanotechnology 18, 205605 (2007).
Shen, G., Cho, J.H., Yoo, J.K., Yi, G.-C. & Lee, C.J. Synthesis of single-crystal CdS microbelts using a modified thermal evaporation method and their photoluminescence. J. Phys. Chem. B 109, 9294–9298 (2005).
An, B.-G., Chang, Y.W., Kim, H.-R., Lee, G., Kang, M.-J., Park, J.-K. & Pyun, J.-C. Highly sensitive photosensor based on in situ synthesized CdS nanowires. Sens. Actuators, B 221, 884–890 (2015).
An, B.-G., Kim, H.-R., Kang, M.-J., Park, J.-G., Chang, Y.W. & Pyun, J.-C. Chemiluminescent lateral-flow immunoassays by using in-situ synthesis of CdS NW photosensor. Anal. Chim. Acta 927, 99–106 (2016).
Im, J.-H., Kim, H.-R., An, B.-G., Chang, Y.W., Kang, M.-J., Lee, T.-G., Son, J.G., Park, J.-G. & Pyun, J.-C. In situ-synthesized cadmium sulfide nanowire photosensor with a parylene passivation layer for chemiluminescent immunoassays. Biosens. Bioelectron. 92, 221–228 (2017).
Ahn, S.-E., Ji, H.J., Kim, K., Kim, G.T., Bae, C.H., Park, S.M., Kim, Y.-K. & Ha, J.S. Origin of the slow photoresponse in an individual sol-gel synthesized ZnO nanowire. Appl. Phys. Lett. 90, 153106 (2007).
Ghosh, R., Dutta, M. & Basak, D. Self-seeded growth and ultraviolet photoresponse properties of ZnO nanowire arrays. Appl. Phys. Lett. 91, 073108 (2007).
Gu, Y. & Lauhon, L.J. Space-charge-limited current in nanowires depleted by oxygen adsorption. Appl. Phys. Lett. 89, 143102 (2006).
He, J., Lin, Y.H., McConney, M.E., Tsukruk, V.V., Wang, Z.L. & Bao, G. Enhancing UV photoconductivity of ZnO nanobelt by polyacrylonitrile functionalization. J. Appl. Phys. 102, 084303 (2007).
Hong, W.-K., Kim, B.-J., Kim, T.-W., Jo, G., Song, S., Kwon, S.-S., Yoon, A., Stach, E.A. & Lee, T. Electrical properties of ZnO nanowire field effect transistors by surface passivation. Colloids Surf., A 313, 378–382 (2008).
Law, J. & Thong, J. Simple fabrication of a ZnO nanowire photodetector with a fast photoresponse time. Appl. Phys. Lett. 88, 133114 (2006).
Kim, H.-R., An, B.-G., Chang, Y.W., Kang, M.-J., Park, J.-G. & Pyun, J.-C. Highly sensitive in situ-synthesized cadmium sulfide (CdS) nanowire photosensor for chemiluminescent immunoassays. Enzyme Microb. Technol. 133, 109457 (2020).
Wang, Y., Kang, K.-M., Kim, M. & Park, H.-H. Effective oxygen-defect passivation in ZnO thin films prepared by atomic layer deposition using hydrogen peroxide. J. Korean Ceram. Soc. 56, 302–307 (2019).
Kwon, S. & Choi, S.-J. Development of tubing-based stationary liquid-phase enzyme-linked immunosorbent assay. Biochip J. 13, 174–181 (2019).
Truong, C.K.P., Nguyen, T.D.D. & Shin, I.-S. Electrochemiluminescent chemosensors for clinical applications: A review. Biochip J. 13, 203–216 (2019).
Bong, J.-H., Kim, J., Lee, G.-Y., Park, J.-H., Kim, T.-H., Kang, M.-J. & Pyun, J.-C. Fluorescence immunoassay of E. coli using anti-lipopolysaccharide antibodies isolated from human serum. Biosens. Bioelectron. 126, 518–528 (2019).
Bong, J.-H., Yoo, G., Park, M., Kang, M.-J., Jose, J. & Pyun, J.-C. Ultrasonic isolation of the outer membrane of Escherichia coli with autodisplayed Z-domains. Enzyme Microb. Technol. 66, 42–47 (2014).
Mandakhalikar, K.D., Rahmat, J.N., Chiong, E., Neoh, K.G., Shen, L. & Tambyah, P.A. Extraction and quantification of biofilm bacteria: method optimized for urinary catheters. Sci. Rep. 8, 8069 (2018).
Kim, J.I., Lee, G.Y., Ko, H., Kang, M.J. & Pyun, J.-C. Covalent protein immobilization with a parylene-H film for matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom. 27, 1149–1154 (2013).
Kim, J.-I., Noh, J.-Y., Kim, M., Park, J.-M., Song, H.-W., Kang, M.-J. & Pyun, J.-C. Newborn screening by matrix-assisted laser desorption/ionization mass spectrometry based on parylene-matrix chip. Anal. Biochem. 530, 31–39 (2017).
Liaqat, U., Ko, H., Suh, H., Lee, M. & Pyun, J.-C. Surface modification of parylene-N films for the culture of osteoblast-like cells (MG-63). Appl. Surf. Sci 378, 277–285 (2016).
Liaqat, U., Ko, H., Suh, H., Lee, M. & Pyun, J.-C. UV-irradiated parylene surfaces for proliferation and differentiation of PC-12 cells. Enzyme Microb. Technol. 97, 1–10 (2017).
Park, J.-M., Kim, J.-I., Noh, J.-Y., Kim, M., Kang, M.-J. & Pyun, J.-C. A highly sensitive carbapenemase assay using laser desorption/ionization mass spectrometry based on a parylene-matrix chip. Enzyme Microb. Technol. 104, 56–68 (2017).
Ko, H., Choi, Y.-H., Chang, S.-Y., Lee, G.-Y., Song, H.-W., Chang, Y.W., Kang, M.-J. & Pyun, J.-C. Surface modification of parylene-N with UV-treatment to enhance the protein immobilization. Eur. Polym. J. 68, 36–46 (2015).
Park, M., Pyun, J.-C. & Jose, J. Orientation and density control of proteins on solid matters by outer membrane coating: Analytical and diagnostic applications. J. Pharm. Biomed. Anal. 147, 174–184 (2018).
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This work was supported by the National Research Foundation of Korea [grant number: NRF-2020R1A2B5B01002187].
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Kim, HR., Bong, JH., Jung, J. et al. An On-chip Chemiluminescent Immunoassay for Bacterial Detection using in Situ-synthesized Cadmium Sulfide Nanowires with Passivation Layers. BioChip J 14, 268–278 (2020). https://doi.org/10.1007/s13206-020-4305-1
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DOI: https://doi.org/10.1007/s13206-020-4305-1