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Microarray of stimuli-responsive microbeads for duplexed immunoassay

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Abstract

This paper describes a duplexed immunoassay method based on a functional microbeads (μbeads) array. For selective detection of dual analytes, the μbeads were conjugated with a responsive biopolymer, characterized by a different phase transition temperature. Then, the μbeads modified with elastin-like polypeptides (ELPs) were spatially localized in a single microwell through sequential injection with a buffer. Prostate specific antigen (PSA) and mouse immunoglobulin G (IgG) were selected to show the feasibility of duplexed immunoassay. The detection limit of the μbead array with PSA and mouse IgG was 0.05 ng/mL and 0.1 ng/mL, respectively.

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Authors and Affiliations

  1. Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul, 151-742, Korea

    Jonghwan Lee, Jaeyeon Jung, Subeom Park, Jie Chen, Jaeyoo Choi & Jinho Hyun

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  1. Jonghwan Lee
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  2. Jaeyeon Jung
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  3. Subeom Park
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  4. Jie Chen
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  5. Jaeyoo Choi
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  6. Jinho Hyun
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Correspondence to Jinho Hyun.

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Lee, J., Jung, J., Park, S. et al. Microarray of stimuli-responsive microbeads for duplexed immunoassay. BioChip J 5, 158–164 (2011). https://doi.org/10.1007/s13206-011-5209-x

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  • DOI: https://doi.org/10.1007/s13206-011-5209-x

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