Abstract
Cucumber wilt is an important soil borne disease in cucumber production, which seriously affects the development of the cucumber industry. Cleome spinosa also has pharmacological effects such as antibacterial, analgesic, anti-inflammatory, and insect repellent. To study the control effect and mechanism of Cleome spinosa fumigation on cucumber wilt disease, different concentrations of Cleome spinosa fragments were applied on cucumber plants infected with Fusarium oxysporum. Cleome spinosa fumigation significantly reduced the incidence rate of cucumber Fusarium wilt. Under the fumigation treatment of 7.5 g kg−1 Cleome spinosa fragments, the preventive effects were 74.7%. Cleome spinosa fragments fumigation can promote cucumber growth and synthesis of photosynthetic pigments, thereby improving individual plant yield and fruit quality. At 7.5 g kg−1 Cleome spinosa fragments fumigation treatment, the plant height and individual plant yield of cucumber increased by 20.3% and 34.3%, respectively. Cleome spinosa fumigation can enhance the activity of antioxidant enzymes in cucumber, maintain a balance of reactive oxygen species metabolism, and enhance the plant disease resistance. Moreover, Cleome spinosa can also regulate the activities of Mg2+-ATPase and Ca2+-ATPase, enhancing its resistance to Fusarium oxysporum. Moreover, number of bacteria and fungi significantly decreased under Cleome spinosa fumigation. Those results suggested that Cleome spinosa could effectively restrain cucumber Fusarium wilt. This study will provide a new idea for the further use of biological fumigation to prevent soil-borne diseases.
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This work was supported by the National Natural Science Foundation of China (31171792) and the Second Batch of Seed Industry Innovation and Development Projects in Heilongjiang Province.
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XZ and DL performed the overall experiment and data analysis. XM, XJ, RS, ZW, JL, BW and CZ performed the experiment to confirm the results and wrote the manuscript together. YZ designed and managed whole experiments and finalized the manuscript.
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Zhang, X., Meng, X., Jiao, X. et al. Preventive effect of Cleome spinosa against cucumber Fusarium wilt and improvement on cucumber growth and physiology. 3 Biotech 14, 97 (2024). https://doi.org/10.1007/s13205-024-03945-4
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DOI: https://doi.org/10.1007/s13205-024-03945-4