Abstract
Fucoidan is linked to a variety of biological processes. Differences in algae species, extraction, seasons, and locations generate structural variability in fucoidan, affecting its bioactivities. Nothing is known about fucoidan from the brown alga Dictyota bartayresiana, its anti-inflammatory properties, or its inherent mechanism. This study aimed to investigate the anti-inflammatory properties of fucoidan isolated from D. bartayresiana against LPS-induced RAW 264.7 macrophages and to explore potential molecular pathways associated with this anti-inflammatory effects. Fucoidan was first isolated and purified from D. bartayresiana, and then, MTT assay was used to determine the effect of fucoidan on cell viability. Its effects on reactive oxygen species (ROS) formation and apoptosis were also studied using the ROS assay and acridine orange/ethidium bromide fluorescence labelling, respectively. Molecular docking and molecular dynamics simulation studies were performed on target proteins NF-κB and TNF-α to identify the route implicated in these inflammatory events. It was observed that fucoidan reduced LPS-induced inflammation in RAW 264.7 cells. Fucoidan also decreased the LPS-stimulated ROS surge and was found to induce apoptosis in the cells. Molecular docking and molecular dynamics simulation studies revealed that fucoidan’s potent anti-inflammatory action was achieved by obstructing the NF-κB signalling pathway. These findings were particularly noteworthy and novel because fucoidan isolated from D. bartayresiana had not previously been shown to have anti-inflammatory properties in RAW 264.7 cells or to exert its activity by obstructing the NF-κB signalling pathway. Conclusively, these findings proposed fucoidan as a potential pharmaceutical drug for inflammation-related diseases.
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Data availability
The data analyzed during the present study are available from the corresponding author upon reasonable request.
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The authors gratefully acknowledge Vellore Institute of Technology, Vellore, for the support for Research.
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AD and JS conceptualized the research, AD did isolation of compound, investigation, data curation, and original draft preparations, TD performed the laboratory experiments and TR conceptualized the laboratory experiments, VD performed the computational experiments, and JS finally reviewed and supervised the entire study.
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Dubey, A., Dasgupta, T., Devaraji, V. et al. Investigating anti-inflammatory and apoptotic actions of fucoidan concentrating on computational and therapeutic applications. 3 Biotech 13, 355 (2023). https://doi.org/10.1007/s13205-023-03771-0
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DOI: https://doi.org/10.1007/s13205-023-03771-0