Abstract
The present study reviewed and discussed the promising affinity tags for one-step purification and immobilization of recombinant proteins. The approach used to structure this systematic review was The Preferred Reporting Items for Systematic Review and Meta-analysis (PRISMA) methodology. The Scopus and Web of Science databases were used to perform the bibliographic survey by which 267 articles were selected. After the inclusion/exclusion criteria and the screening process, from 25 chosen documents, we identified 7 types of tags used in the last 10 years, carbohydrate-binding module tag (CBM), polyhistidine (His-tag), elastin-like polypeptides (ELPs), silaffin-3-derived pentalysine cluster (Sil3k tag), N-acetylmuramidase (AcmA tag), modified haloalkane dehalogenase (HaloTag®), and aldehyde from a lipase polypeptide (Aldehyde tag). The most used bacterial host for expressing the targeted protein was Escherichia coli and the most used expression vector was pET-28a. The results demonstrated two main immobilization and purification methods: the use of supports and the use of self-aggregating tags without the need of support, depending on the tag used. Besides, the chosen terminal for cloning the tag proved to be very important once it could alter enzyme activity. In conclusion, the best tag for protein one-step purification and immobilization was CBM tag, due to the eco-friendly supports that can be provided from industry wastes, the fast immobilization with high specificity, and the reduced cost of the process.
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Funding
We would like to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (grant no. 308515/2020–0, no. 306010/2021–6), and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES) for the scholarships granted, and the University of Vale do Taquari—Univates and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS) (grant no. 22/2551–0000397-4 (Bioprocess and Biotechnology for Food Research Center—Biofood)) for the financial support granted for this research paper. This study was partially funded by CAPES – Finance Code 001.
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All authors contributed to the study conception and design. FL, AG, GBP, CS, FXS, DCDAM, and GR: writing—review and editing. GV and CFVS: writing—review and editing, project administration. All authors read and approved the manuscript.
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Leonhardt, F., Gennari, A., Paludo, G.B. et al. A systematic review about affinity tags for one-step purification and immobilization of recombinant proteins: integrated bioprocesses aiming both economic and environmental sustainability. 3 Biotech 13, 186 (2023). https://doi.org/10.1007/s13205-023-03616-w
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DOI: https://doi.org/10.1007/s13205-023-03616-w