Abstract
In the study, a previously isolated plant beneficial endophytic B. cereus CaB1 was selected for the detailed analysis by whole-genome sequencing. The WGS has generated a total of 1.9 GB high-quality data which was assembled into a 5,257,162 bp genome with G + C content of 35.2%. Interestingly, CaB1 genome was identified to have 40 genes with plant beneficial functions by bioinformatic analysis. At the same time, it also showed the presence of various virulence factors except the diarrhoeal toxin, cereulide. Upon comparative analysis of CaB1 with other B. cereus strains, it was found to have random distributions of virulence and plant growth promoting traits. The core genome phylogenetic analysis of the Bacillus cereus strains further showed the close relation of plant associated strains with isolates from spoiled food products. The observed genome flexibility of B. cereus thus indicates its ability to make use of diverse hosts, which can result either in beneficial or harmful effects.
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The datasets analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
Acknowledging Miss. Jilna Babu and Miss. Celene Francis research assistants at Center for Bioinformatics, School of Biosciences, Mahatma Gandhi University.
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SS: conceptualization, data analysis, writing; MP: data analysis, SBM: writing, CGI: writing, CSB: data analysis, AK: Software and whole genome analysis; RB: software and whole genome analysis; SNS: conceptualization, review and editing; EKR: conceptualization, supervision and editing.
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Sreejith, S., Paul, M., Mol, S.B. et al. Genomic insight into the environmental adaptations and toxigenic features of endophytic Bacillus cereus CaB1 isolated from Capsicum annuum L.. 3 Biotech 13, 68 (2023). https://doi.org/10.1007/s13205-023-03463-9
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DOI: https://doi.org/10.1007/s13205-023-03463-9