Abstract
The level of clotting protein ‘factor IX’ (FIX) is highly associated with cerebral ischemia, and this research work has developed a sensitive detection of FIX on dielectrode sensor by current-volt measurement. Sensing area was grown with zinc oxide nanowire to attach more probe for FIX interaction. Aptamer was utilized as the detection probe and attached on the sensing electrode surface through amine-aldehyde chemical linkage. In addition, biotin-streptavidin interaction was utilized to attach the higher number aptamers on the electrode surface connected with dual-probe station. FIX detection limit was found as 10 fM in the phosphate buffer saline spiked samples and 1:320 dilution of human serum. The linear ranges were as 10 fM to 100 pM and 1:320 to 1:80, respectively. With a good determination co-efficient [y = 2.6813x − 3.8467; R2 = 0.9479] this biosensing strategy helps to quantify FIX and monitor the condition of cerebral ischemia.
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WG, MS and XL conceived and designed the study. XG, YL, HL and LX performed the literature search and data extraction. WX drafted the manuscript. The authors are contributed to the preparation of the manuscript and discussion. All authors read and approved the final manuscript.
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Gong, W., Sun, M., Guo, X. et al. Nanowired dual-electrodes surface to monitor cerebral ischemia by current-volt measurements. 3 Biotech 11, 502 (2021). https://doi.org/10.1007/s13205-021-03048-4
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DOI: https://doi.org/10.1007/s13205-021-03048-4