Abstract
In this study, the putative genes involved in diterpenoid alkaloids biosynthesis in A. vilmorinianum roots were revealed by transcriptome sequencing. 59.39 GB of clean bases and 119,660 unigenes were assembled, of which 69,978 unigenes (58.48%) were annotated. We identified 27 classes of genes (139 candidate genes) involved in the synthesis of diterpenoid alkaloids, including the mevalonate (MVA) pathway, the methylerythritol 4-phosphate (MEP) pathway, the farnesyl diphosphate regulatory pathway, and the diterpenoid scaffold synthetic pathway. 12 CYP450 genes were identified. We found that hydroxymethylglutaryl-CoA reductase was the key enzyme in MVA metabolism, which was regulated by miR6300. Transcription factors, such as bHLH, AP2/EREBP, and MYB, used to synthesize the diterpenes were analyzed.
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Acknowledgements
This work was supported in part by the National Natural Science Foundation of China (No. 31560351; No. 31760349).
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All authors read and approved the final manuscript. Y-GL conceived and designed the experiments, performed the experiments, wrote the paper, read and approved the final manuscript. F-JM contributed reagents, materials, analysis tools, read and approved the final manuscript. K-ZL conceived and designed the experiment, read and approved the final manuscript.
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Li, YG., Mou, FJ. & Li, KZ. De novo RNA sequencing and analysis reveal the putative genes involved in diterpenoid biosynthesis in Aconitum vilmorinianum roots. 3 Biotech 11, 96 (2021). https://doi.org/10.1007/s13205-021-02646-6
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DOI: https://doi.org/10.1007/s13205-021-02646-6