Skip to main content
SpringerLink
Log in

OsPLB gene expressed during seed germination encodes a phospholipase in rice

  • Original Article
  • Published:
3 Biotech Aims and scope Submit manuscript

Abstract

Hydrolysis of phospholipid monolayer by phospholipases is an important event in the mobilization of stored lipids for seed germination. However, the identification and functional characterization of cereal phospholipases, especially during rice germination, are limited. In the present study, we have identified and characterized a phospholipase OsPLB gene expressed during germination. The full-length coding region of OsPLB was cloned into pRSETA as well as pYES2/NTC vector. The recombinant protein was successfully expressed in both E. coli and Saccharomyces cerevisiae. The recombinant protein was purified to homogeneity by affinity chromatography, and it was further confirmed by MS/MS analysis. In vitro lipase assay and lipidome analysis using high-resolution mass spectrometry showed phosphatidylcholine (PC) specific phospholipase B activity. The results revealed that protein encoded by OsPLB gene prefers to hydrolyze PCs with C28, C32, and C34 containing unsaturated fatty acids. Collectively, the present study describes the identification and characterization of a phospholipase B, which hydrolyze PC, a major component of phospholipid monolayer covering storage lipid, as an initial event during rice seed germination.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Referrences

  • Aizono Y, Funatsu M, Sugano M et al (1973) Enzymatic properties of rice bran lipase. Agric Biol Chem 37:2031–2036

    Article  CAS  Google Scholar 

  • Bayon S, Chen G, Weselake RJ et al (2015) A small phospholipase A2-α from castor catalyzes the removal of hydroxy fatty acids from phosphatidylcholine in transgenic arabidopsis seeds. Plant Physiol 167:1259–1270

    Article  CAS  Google Scholar 

  • Bhardwaj K, Raju A, Rajasekharan R (2001) Identification, purification, and characterization of a thermally stable lipase from rice bran. A new member of the (Phospho) lipase family. Plant Physiol 127:1728–1738

    Article  CAS  Google Scholar 

  • Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917

    Article  CAS  Google Scholar 

  • Borgston B, Brockman HL (1984) Lipases. Elsevier, Amsterdam

    Google Scholar 

  • Chepyshko H, Lai CP, Huang LM et al (2012) Multifunctionality and diversity of GDSL esterase/lipase gene family in rice (Oryza sativa L. japonica) genome: new insights from bioinformatics analysis. BMC Genom 13:309

    Article  CAS  Google Scholar 

  • Chuang HH, Chen PT, Wang WN et al (2011) Functional proteomic analysis of rice bran esterases/lipases and characterization of a novel recombinant esterase. J Agric Food Chem 59:2019–2025

    Article  CAS  Google Scholar 

  • Denev R, Kuzmanova I, Panayotova S et al (2009) Lipid composition of Indian rice bran oil. Chimieorganique 62:709–716

    CAS  Google Scholar 

  • Eastmond PJ (2006) Sugar-dependent1 encodes a patatin domain triacylglycerol lipase that initiates storage oil breakdown in germinating arabidopsis seeds. Plant Cell 18:665–675

    Article  CAS  Google Scholar 

  • Fauconnier M-L, Welti R, Blée E, Marlier M (2003) Lipid and oxylipin profiles during aging and sprout development in potato tubers (Solanum tuberosum L.). Biochim Biophys Acta Mol Cell Biol Lipids 1633:118–126

    Article  CAS  Google Scholar 

  • Fruehan C, Johnson D, Welti R (2018) Lipidomedbb data calculation environment has been updated to process direct-infusion multiple reaction monitoring data. Lipids 53:1019–1020

    Article  CAS  Google Scholar 

  • Glushenkova AI, Ul’chenko NN, Talipova M et al (1998) Lipids of rice bran. Chem Nat Compd 34:275–277

    Article  CAS  Google Scholar 

  • Graham IA (2008) Seed storage oil mobilization. Annu Rev Plant Biol 59:115–142

    Article  CAS  Google Scholar 

  • Gupta A, Bhatla SC (2007) Preferential phospholipase A2 activity on the oil bodies in cotyledons during seed germination in Helianthus annuus L. cv. Morden. Plant Sci 172:535–543

    Article  CAS  Google Scholar 

  • Heier C, Taschler U, Rengachari S et al (2010) Identification of Yju3p as functional orthologue of mammalian monoglyceride lipase in the yeast Saccharomyces cerevisiae. Biochim Biophys Acta Mol Cell Biol Lipids 1801:1063–1071

    Article  CAS  Google Scholar 

  • Kim Y (2004) Cloning and expression of a lipase gene from rice (Oryza sativacv. Dongjin). Mol Cell 18:40–45

    CAS  Google Scholar 

  • Li G, Lin F, Xue HW (2007) Genome-wide analysis of the phospholipase D family in Oryza sativa and functional characterization of PLDβ1 in seed germination. Cell Res 17:881–894

    Article  CAS  Google Scholar 

  • Lin YH, Huan AHC (1984) Purification and initial characterization of lipase from the scutella of corn seedlings. Plant Physiol 76:719–722

    Article  CAS  Google Scholar 

  • Lin Y, Xin X, Yin G et al (2019) Membrane phospholipids remodeling upon imbibition in Brassica napus L. seeds. Biochem Biophys Res Commun 515:289–295

    Article  CAS  Google Scholar 

  • May C, Preisig-Müller R, Höhne M et al (1998) A phospholipase A2 is transiently synthesized during seed germination and localized to lipid bodies. Biochim Biophys Acta Lipids Lipid Metab 1393:267–276

    Article  CAS  Google Scholar 

  • Minasbekyan LA, Yavroyan ZV, Darbinyan MR et al (2004) The phospholipid composition of nuclear subfractions from germinating wheat embryos. Russ J Plant Physiol 51:708–712

    Article  CAS  Google Scholar 

  • Murphy DJ (2001) The biogenesis and functions of lipid bodies in animals, plants and microorganisms. Prog Lipid Res 40:325–438

    Article  CAS  Google Scholar 

  • Palmiano EP, Juliano BO (1973) Changes in the activity of some hydrolases, peroxidase, and catalase in the rice seed during germination. Plant physl 52:274–277

    Article  CAS  Google Scholar 

  • Parthibane V, Rajakumari S, Venkateshwari V et al (2011) Oleosin is bifunctional enzyme that has both monoacylglycerol acyltransferase and phospholipase activities. J Biol Chem 287:1946–1954

    Article  Google Scholar 

  • Rietz S, Dermendjiev G, Oppermann E et al (2010) Roles of arabidopsis patatin-related phospholipases A in root development are related to auxin responses and phosphate deficiency. Mol Plant 3:524–538

    Article  CAS  Google Scholar 

  • Seo YS, Kim EY, Kim WT (2011) The Arabidopsis sn-1-specific mitochondrial acylhydrolase AtDLAH is positively correlated with seed viability. J Exp Bot 62:5683–5698

    Article  CAS  Google Scholar 

  • Shevchenko A, Tomas H, Havlis J et al (2007) In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nat Protoc 1:2856–2860

    Article  Google Scholar 

  • Tsai HC, Chung KR (2014) Calcineurin phosphatase and phospholipase C are required for developmental and pathological functions in the citrus fungal pathogen Alternaria alternata. Microbiology 160:1453–1465

    Article  CAS  Google Scholar 

  • Vicient CM, Delseny M (1999) Isolation of total RNA from Arabidopsis thaliana seeds. Anal Biochem 268:412–413

    Article  CAS  Google Scholar 

  • Welti R, Wang X (2004) Lipid species profiling: a high-throughput approach to identify lipid compositional changes and determine the function of genes involved in lipid metabolism and signaling. Curr Opin Plant Biol 7:337–344

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank Dr. A. Jayadeep, CSIR-CFTRI for providing the fresh O. sativa (IR64) seeds for the study. We are grateful to Prof. Ram Rajasekharan for his constant support and encouragement. We appreciate Dr. Arun Kumar V, CSIR-CFTRI for his constructive criticism of the manuscript. Funding was provided by Department of Science and Technology, Govt of India (Grant no. IFA14-LSPA28).

Author information

Author notes

  1. Achintya Kumar Dolui and Mahadev Latha contributed equally to the article.

Authors and Affiliations

  1. Department of Lipid Science, CSIR-Central Food Technological Research Institute, Mysuru, Karnataka, 570020, India

    Achintya Kumar Dolui, Mahadev Latha & Panneerselvam Vijayaraj

  2. Academy of Scientific and Innovative Research, Ghaziabad, Uttar Pradesh, 201002, India

    Achintya Kumar Dolui, Mahadev Latha & Panneerselvam Vijayaraj

Authors
  1. Achintya Kumar Dolui
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mahadev Latha
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Panneerselvam Vijayaraj
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

PV conceived the original research plans and supervised the experiments. AKD and ML carried out all the experiments. PV and AKD analyzed and discussed the data. PV and AKD wrote the manuscript.

Corresponding author

Correspondence to Panneerselvam Vijayaraj.

Ethics declarations

Conflict of interest

Authors have no conflict of interest to declare.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 384 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dolui, A.K., Latha, M. & Vijayaraj, P. OsPLB gene expressed during seed germination encodes a phospholipase in rice. 3 Biotech 10, 30 (2020). https://doi.org/10.1007/s13205-019-2016-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s13205-019-2016-x

Keywords

Search

Navigation