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Responses of microbial community to different concentration of perchlorate in the Qingyi River

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Abstract

Perchlorate is a refractory and mobile contaminant that is wildly distributed in surface water, and due to its tremendous inhibitory effect on mammalian thyroid function, it has gained much attention in recent years. Numerous studies have focused on environmental detection of perchlorate, especially in water. However, less attention has been paid to the effects of perchlorate on the composition of the microbial community in rivers. Upstream of the Qingyi River, an important source of drinking water for local residents, there are two perchlorate manufacturers. In this study, we selected eight study sites from upstream to downstream of the Qingyi River, including sites located upstream and downstream of the perchlorate manufacturers. Our results indicated that perchlorate was detected in all sites except for QYR2, QYR3, and QYR10. The concentration of perchlorate in the five study sites was much higher than the reference dose proposed by the National Academy of Science, and ranged from 187 to 9647.00 μg/L. We utilized 16S rDNA high throughput sequencing to analyze changes in the composition of the microbial community, based on the Illumina 2 × 250 MiSeq platform. The results showed that, when microbial communities were exposed to high concentration of perchlorate, there was an increase in the ratio of Betaproteobacteria, Bacteroidetes, Actinobacteria, and Saccharibacteria in the microbial community along with a decrease in the ratio of Chloroflexi, Verrucomicrobia, and Gammaproteobacteria. Our study has provided a theoretical basis for the alteration of the microbial community caused by the perchlorate pollution, which maybe have a truly important impact on the quality of groundwater.

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Acknowledgements

This study was supported by the Sichuan Environmental Protection Science and Technology Project (2018HB27) and the Ya'an Science and Technology Plan (2016yyjsykf005).

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Author notes

  1. Jingzhou Sha and Jianqin Wu contributed equally to this work.

Authors and Affiliations

  1. Sichuan Solid Waste and Chemical Management Center, Chengdu, Sichuan, China

    Jingzhou Sha & Chaowen Bi

  2. Experimental Animal Center, Shanxi Medical University, Shanxi, China

    Jianqin Wu

  3. Shanxi Key Laboratory of Laboratory Animal and Animal Model of Human Diseases, Shanxi Medical University, Shanxi, China

    Jianqin Wu

  4. Yaan Environmental Monitoring Center, Yaan, Sichuan, China

    Jingzhou Sha, Changxu Chen, Qiang Su, Songtao Wang, Chuan Wang & Yuren Zhou

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  1. Jingzhou Sha
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Correspondence to Chaowen Bi.

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Sha, J., Wu, J., Bi, C. et al. Responses of microbial community to different concentration of perchlorate in the Qingyi River. 3 Biotech 10, 21 (2020). https://doi.org/10.1007/s13205-019-2012-1

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