Abstract
Medicinally important ancient Navara rice (GI Kerala, India 2007) is a very short duration (60–70 days) variety with a yield of only 0.5 tonnes/hectare costing ~ Rs. 400/kg. It is used for indigenous treatment for chronic diseases by local and oral consumption. In this study, scutellum-derived calli were generated from mature Navara seeds and these were inoculated on different CIM-1 to CIM-5 media supplemented with 2.5 mg/l 2,4-dichlorophenoxyacetic acid. Regeneration of calli on different regeneration media RI, RII and RIII media were performed. Regeneration of 30-day-old calli on RI media showed 30%, for RII it showed no regeneration and on RIII media only 12% regeneration was obtained. The addition of glutamine and proline showed a 30–40% improvement in somatic embryogenesis. The 74–88% callus induction frequency was obtained on CIM-1 to CIM-5. The fresh weight (mg) of 30-day-old calli is CIM-2 < CIM-3 < CIM-4 < CIM-1 << CIM-5 and corresponding size shows CIM-2–CIM-3 < CIM-5 < CIM-1 < CIM-4. A negative correlation between the callus fresh weight and the regeneration efficiency was observed. In CIM-5, 20–25 days 3.4-fold increase and 25–30 days a 1.7-fold increase in fresh weight of calli is noted. The 20-day-old calli transfer to RI media shows 80% regeneration frequency and 6–7 plantlets/callus, which are twofold higher as compared with 30-day-old calli. The somatic embryogenesis and its regeneration on synthetic media provide an alternative for biotechnological intervention for yield improvement, in turn cost reduction for Navara rice.
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Change history
09 August 2019
In the original article, on the page 270 the increasing order of fresh weight of calli is for the 10 days post-inoculation as against 15 days mentioned in the text (Table 4). The authors regret the mistake. Authors thank Mr. Haifang Jin, for critically reading the article and enabling us to provide this correction.
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Acknowledgements
MS, AS and LIS are grateful to the Department of Biotechnology, India for research grant BT/PR11797/AGR/36/608/2009, MS and AS also thank University Grant Commission (321154, F.25-1/2013-14(BSR)/7-210/2009(BSR)), India for providing SRF. Dr. Shinoj Subramanian (Sr. Scientist), Krishi Vigyan Kendra, Ernakulam, Kerala, India is gratefully acknowledged for providing Navara seeds. Mr Bhanu Prakash R (Scientific advisor M/s. SCIENCE4U Analytics and Research solutions PVT Ltd, Bangalore) is gratefully acknowledged for helping us with “The Unscramble X” software data analysis.
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Solanki, M., Sinha, A. & Shukla, L.I. Optimization of in vitro culture media for improvement in yield of Navara ancient Indian medicinal rice. 3 Biotech 9, 270 (2019). https://doi.org/10.1007/s13205-019-1797-2
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DOI: https://doi.org/10.1007/s13205-019-1797-2