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Synergistic effect of thermostable β-glucosidase TN0602 and cellulase on cellulose hydrolysis

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Abstract

Thermophilic enzymes have many potential benefits in industrial production with increased flexibility related to process configurations. A thermostable β-glucosidase from Thermotoga naphthophila RUK-10 was found to possess catalytic activity for cellobiose hydrolysis with a high potential for application in biomass conversion. The aggregation of cellobiose often has an inhibitory effect on cellobiohydrolases and endoglucanases during cellulose hydrolysis. The presence of β-glucosidases has a significant effect on reducing inhibition from hydrolytic products by hydrolysing the intermedia cellobiose. In this study, β-glucosidase TN0602 exhibited a high tolerance to glucose and high thermostability even after a long incubation (>72 h). Additionally, supplementing β-glucosidase TN0602 with microcrystalline cellulose, untreated corn straw and steam-exploded corn straw hydrolysis reactions containing a commercial cellulase led to an increased conversion rate in released glucose compared to hydrolysis without the addition of β-glucosidase (15.82, 30.62 and 35.21%, respectively); the increase of conversion rates were 61.86, 93.50 and 94.55%. It was thus shown that an obvious synergistic effect exists between TN0602 and cellulases for cellulose hydrolysis, suggesting its potential as a component of enzymatic cocktails for the conversion of lignocellulosic biomass to other chemicals.

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Acknowledgements

First author thanks Jilin Agriculture University, College of Life Science, Changchun and Jilin University, Key Laboratory for Molecular Enzymology and Engineering Ministry of Education for providing research facility. The authors gratefully acknowledge the financial support from the National Natural Science Foundation of China (nos. 20772046 and 21072075) and High-end technology innovation platform of straw comprehensive utilization of colleges and universities in Jilin province ((2014) C–1).

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Authors and Affiliations

  1. College of Life Science, Jilin Agriculture University, 130118, Changchun, China

    Zhaoye Zhang, Xiaoxiao Yu & Guang Chen

  2. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun, 130012, China

    Man Wang & Renjun Gao

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  1. Zhaoye Zhang
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  2. Man Wang
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  3. Renjun Gao
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  4. Xiaoxiao Yu
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  5. Guang Chen
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Correspondence to Xiaoxiao Yu or Guang Chen.

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Zhang, Z., Wang, M., Gao, R. et al. Synergistic effect of thermostable β-glucosidase TN0602 and cellulase on cellulose hydrolysis. 3 Biotech 7, 54 (2017). https://doi.org/10.1007/s13205-017-0672-2

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