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Inoculation with Pseudomonas putida PCI2, a phosphate solubilizing rhizobacterium, stimulates the growth of tomato plants

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Abstract

The use of phosphate solubilizing plant growth-promoting microorganisms as inoculants assists in the hydrolysis of insoluble forms of phosphorus leading to increased plant growth. Pseudomonas putida PCI2 was evaluated for phosphatase activity and solubilization of AlPO4 and FePO4. The effect of different incubation temperatures, concentrations of NaCl and different pH on growth of PCI2 and P solubilization was studied. PCI2 proved to be positive for phosphatase activity, solubilized AlPO4 and hydrolyzed Ca3(PO4)2 even in medium with 5 % NaCl. In addition, PCI2 produced 45 % units of siderophores. The production of IAA by PCI2 was stimulated in vitro by the addition of different concentrations of L-tryptophan to the culture medium. Assays with tomato seedlings showed that the length of the root was reduced as the concentration of IAA increased. On the other hand, inoculation with PCI2 caused a clear growth-promoting effect on shoot growth in the presence of L-tryptophan. P. putida PCI2 is adapted to different environmental conditions and has potential to be developed and used as an inoculant for increasing the growth of tomato plants.

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Acknowledgments

This work was supported by grants from Secretaría de Ciencia y Técnica de la Universidad Nacional de Río Cuarto (Córdoba, Argentina), Agencia Nacional de Promoción Científica y Tecnológica (Secretaría de Ciencia y Técnica de la Nación) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, Argentina).

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Authors and Affiliations

  1. Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina

    Nicolás Pastor & Susana Rosas

  2. Departamento de Ciencias Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina

    Virginia Luna

  3. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36, Km 601. CP X5804BYA, Río Cuarto, Córdoba, Argentina

    Marisa Rovera

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  1. Nicolás Pastor
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  4. Marisa Rovera
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Correspondence to Marisa Rovera.

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Pastor, N., Rosas, S., Luna, V. et al. Inoculation with Pseudomonas putida PCI2, a phosphate solubilizing rhizobacterium, stimulates the growth of tomato plants. Symbiosis 62, 157–167 (2014). https://doi.org/10.1007/s13199-014-0281-3

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