Abstract
The need for high-quality dietary proteins has risen over the years with improvements in the quality of life. Deep eutectic solvents (DESs) have been regarded as potential green alternatives to conventional organic solvents for protein extraction from press cake biomass, meeting the needs of sustainable development goals. Sacha inchi seed meal (SIM) is generated as a by-product of the seed oil extraction industries containing high protein content. The current study presents a novel ultrasound assisted DES method for the extraction of SIM protein in a sequential manner. Four different DESs were screened, out of which choline chloride (ChCl)/glycerol (1:2) gave promising results in protein recovery and was further selected. The sequential ultrasound-ChCl/glycerol could effectively extract high total crude protein content (77.43%) from SIM biomass compared to alone ultrasound (29.21%) or ChCl/glycerol (58.32%) treatment strategies. The SIM protein extracted from ultrasound-ChCl/glycerol exhibited high solubility (94.39%) at alkaline pH and highest in vitro digestibility (71.16%) by digestive enzymes (pepsin and trypsin). The protein characterization by SDS-PAGE and FTIR elucidated the structural properties and presence of different functional groups of SIM protein. Overall, the sequential ultrasound-ChCl/glycerol revealed its significant potential for one-step biorefining of the waste Sacha inchi meal biomass for circular bioeconomy.
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The data used and analyzed in the present work are available from the corresponding author on a reasonable request.
Abbreviations
- DES:
-
Deep eutectic solvent
- FTIR:
-
Fourier transform infrared spectroscopy
- SIM:
-
Sacha inchi meal
- ChCl:
-
Choline chloride
- PEG:
-
Polyethylene glycol
- RPM:
-
Rotations per minute
- NaoAc:
-
Sodium acetate
- SDS-PAGE :
-
Sodium dodecyl-sulfate polyacrylamide gel electrophoresis
References
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254. https://doi.org/10.1016/0003-2697(76)90527-3
Chen Q, Chaihu L, Yao X et al (2021) Molecular property-tailored soy protein extraction process using a deep eutectic solvent. ACS Sustain Chem Eng 9:10083–10092. https://doi.org/10.1021/acssuschemeng.1c01848
Chirinos R, Aquino M, Pedreschi R, Campos D (2017) Optimized methodology for alkaline and enzyme-assisted extraction of protein from Sacha Inchi (Plukenetia volubilis) kernel cake. J Food Process Eng 40:e12412. https://doi.org/10.1111/jfpe.12412
Du M, Xie J, Gong B et al (2018) Extraction, physicochemical characteristics and functional properties of Mung bean protein. Food Hydrocoll 76:131–140. https://doi.org/10.1016/j.foodhyd.2017.01.003
Fetzer A, Herfellner T, Stäbler A et al (2018) Influence of process conditions during aqueous protein extraction upon yield from pre-pressed and cold-pressed rapeseed press cake. Ind Crops Prod 112:236–246. https://doi.org/10.1016/j.indcrop.2017.12.011
Grudniewska A, de Melo EM, Chan A et al (2018) Enhanced protein extraction from oilseed cakes using glycerol-choline chloride deep eutectic solvents: a biorefinery approach. ACS Sustain Chem Eng 6:15791–15800. https://doi.org/10.1021/acssuschemeng.8b04359
Guzmán-Lorite M, Marina ML, García MC (2022) Successive extraction using natural deep eutectic solvents and pressurized liquids for a greener and holistic recovery of proteins from pomegranate seeds. Food Res Int 161:111862. https://doi.org/10.1016/j.foodres.2022.111862
Hadidi M, Ibarz A, Pouramin S (2021) Optimization of extraction and deamidation of edible protein from evening primrose (Oenothera biennis L.) oil processing by-products and its effect on structural and techno-functional properties. Food Chem 334:127613. https://doi.org/10.1016/j.foodchem.2020.127613
Horwitz W (2000) Official methods of analysis of AOAC International, 17th edn. AOAC International, Gaithersburg
Karabiber EB, Yılmaz E (2017) Extraction and characterisation of lemon, orange and grapefruit seeds press cake proteins. Qual Assur Saf 9:357–367. https://doi.org/10.3920/QAS2016.0984
Li Y, Cheng Y, Zhang Z et al (2020) Modification of rapeseed protein by ultrasound-assisted pH shift treatment: Ultrasonic mode and frequency screening, changes in protein solubility and structural characteristics. Ultrason Sonochem 69:105240. https://doi.org/10.1016/j.ultsonch.2020.105240
Lin J, Xiang H, Sun-Waterhouse D et al (2022) Deep eutectic solvents and alkaline extraction of protein from seabuckthorn seed meal: a comparison study. Food Sci Hum Wellness 11:1028–1035. https://doi.org/10.1016/j.fshw.2022.03.019
Martínez-Padilla LP, Hernández-Rojas FS, Sosa-Herrera MG, Juliano P (2022) Novel application of ultrasound and microwave-assisted methods for aqueous extraction of coconut oil and proteins. J Food Sci Technol 59:3857–3866. https://doi.org/10.1007/s13197-022-05409-0
Nargotra P, Sharma V, Gupta M et al (2018) Application of ionic liquid and alkali pretreatment for enhancing saccharification of sunflower stalk biomass for potential biofuel-ethanol production. Bioresour Technol 267:560–568. https://doi.org/10.1016/j.biortech.2018.07.070
Nargotra P, Sharma V, Sharma S et al (2020) Development of consolidated bioprocess for biofuel-ethanol production from ultrasound-assisted deep eutectic solvent pretreated Parthenium hysterophorus biomass. Biomass Conv Bioref 10:1–16. https://doi.org/10.1007/s13399-020-01017-0
Nargotra P, Sharma V, Sharma S et al (2022) Purification of an ionic liquid stable cellulase from Aspergillus aculeatus PN14 with potential for biomass refining. Environ Sustain 5:313–323. https://doi.org/10.1007/s42398-022-00232-x
Nargotra P, Sharma V, Lee Y-C et al (2023) Microbial Lignocellulolytic enzymes for the effective valorization of lignocellulosic biomass: a review. Catalysts 13:83. https://doi.org/10.3390/catal13010083
Nargotra P, Sharma V, Bajaj BK (2019) Consolidated bioprocessing of surfactant-assisted ionic liquid-pretreated Parthenium hysterophorus L. biomass for bioethanol production. Bioresour Technol 289:121611. https://doi.org/10.1016/j.biortech.2019.121611
Özyurt VH, Tetik I, Ötleş S (2021) Influence of process conditions on ultrasound-assisted protein extraction from cold pressed tomato seed waste. J Food Process Preserv 45:e16079. https://doi.org/10.1111/jfpp.16079
Parodi E, La Nasa J, Ribechini E et al (2021) Extraction of proteins and residual oil from flax (Linum usitatissimum), camelina (Camelina sativa), and sunflower (Helianthus annuus) oilseed press cakes. Biomass Conv Bioref. https://doi.org/10.1007/s13399-021-01379-z
Polmann G, Rossi GB, Teixeira GL et al (2022) High-added value co-products obtained from pecan nut (Carya illinoinensis) using a green extraction technology. J Food Sci Technol 59:2284–2294. https://doi.org/10.1007/s13197-021-05242-x
Rawdkuen S, D’Amico S, Schoenlechner R (2022) Physicochemical, functional, and in vitro digestibility of protein isolates from Thai and Peru Sacha Inchi (Plukenetia volubilis L.) oil press-cakes. Foods 11:1869. https://doi.org/10.3390/foods11131869
Saatchi A, Kiani H, Labbafi M (2019) A new functional protein-polysaccharide conjugate based on protein concentrate from sesame processing by-products: functional and physico-chemical properties. Int J Biol Macromol 122:659–666. https://doi.org/10.1016/j.ijbiomac.2018.10.122
Sharma V, Bhat B, Gupta M et al (2018) Role of systematic biology in biorefining of lignocellulosic residues for biofuels and chemicals production. In: Singh OV, Chandel AK (eds) Sustainable biotechnology- enzymatic resources of renewable energy. Springer, Cham, pp 5–55
Sharma V, Nargotra P, Bajaj BK (2019) Ultrasound and surfactant assisted ionic liquid pretreatment of sugarcane bagasse for enhancing saccharification using enzymes from an ionic liquid tolerant Aspergillus assiutensis VS34. Bioresour Technol 285:121319. https://doi.org/10.1016/j.biortech.2019.121319
Sharma V, Nargotra P, Sharma S, Bajaj BK (2020) Efficient bioconversion of sugarcane tops biomass into biofuel-ethanol using an optimized alkali-ionic liquid pretreatment approach. Biomass Conv Bioref 10:1–14. https://doi.org/10.1007/s13399-020-01123-z
Sharma S, Nargotra P, Sharma V et al (2021a) Nanobiocatalysts for efficacious bioconversion of ionic liquid pretreated sugarcane tops biomass to biofuel. Bioresour Technol 333:125191. https://doi.org/10.1016/j.biortech.2021.125191
Sharma V, Nargotra P, Sharma S, Bajaj BK (2021b) Efficacy and functional mechanisms of a novel combinatorial pretreatment approach based on deep eutectic solvent and ultrasonic waves for bioconversion of sugarcane bagasse. Renew Energy 163:1910–1922. https://doi.org/10.1016/j.renene.2020.10.101
Sharma V, Tsai M-L, Chen C-W et al (2022a) Deep eutectic solvents as promising pretreatment agents for sustainable lignocellulosic biorefineries: a review. Bioresour Technol 360:127631. https://doi.org/10.1016/j.biortech.2022.127631
Sharma V, Tsai M-L, Nargotra P et al (2022b) Agro-industrial food waste as a low-cost substrate for sustainable production of industrial enzymes: a critical review. Catalysts 12:1373. https://doi.org/10.3390/catal12111373
Sharma V, Tsai M-L, Nargotra P et al (2022c) Journey of lignin from a roadblock to bridge for lignocellulose biorefineries: a comprehensive review. Sci Total Environ. https://doi.org/10.1016/j.scitotenv.2022.160560
Sharma S, Tsai M-L, Sharma V et al (2023a) Environment friendly pretreatment approaches for the bioconversion of lignocellulosic biomass into biofuels and value-added products. Environments 10:6. https://doi.org/10.3390/environments10010006
Sharma V, Nargotra P, Sharma S et al (2023b) Microwave irradiation-assisted ionic liquid or deep eutectic solvent pretreatment for effective bioconversion of sugarcane bagasse to bioethanol. Energy Ecol Environ 8:1–16. https://doi.org/10.1007/s40974-022-00267-0
Singh S, Gupta P, Sharma V et al (2014) Multifarious potential applications of keratinase of Bacillus subtilis K-5. Biocatal Biotransform 32:333–342. https://doi.org/10.3109/10242422.2014.978306
Weng Z-H, Nargotra P, Kuo C-H, Liu Y-C (2022) Immobilization of Recombinant Endoglucanase (CelA) from Clostridium thermocellum on Modified Regenerated Cellulose Membrane. Catalysts 12:1356. https://doi.org/10.3390/catal12111356
Xia N, Wang J-M, Gong Q et al (2012) Characterization and In Vitro digestibility of rice protein prepared by enzyme-assisted microfluidization: comparison to alkaline extraction. J Cereal Sci 56:482–489. https://doi.org/10.1016/j.jcs.2012.06.008
Yang K, Xu T-R, Fu Y-H et al (2021) Effects of ultrasonic pre-treatment on physicochemical properties of proteins extracted from cold-pressed sesame cake. Food Res Int 139:109907. https://doi.org/10.1016/j.foodres.2020.109907
Zhou Y, Wu W, Zhang N et al (2022) Deep eutectic solvents as new media for green extraction of food proteins: opportunity and challenges. Food Res Int 161:111842. https://doi.org/10.1016/j.foodres.2022.111842
Acknowledgements
Authors VS and PN thankfully acknowledge National Kaohsiung University of Science and Technology, Kaohsiung City, Taiwan, for providing the Post-Doctoral fellowship. Authors MLT, CWC, CDD acknowledge the Taiwan MOST for funding support (Ref. No. 109-2222-E-992-002).
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This study was financially supported by Taiwan MOST (Ref. No. 109-2222-E-992-002).
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VS conceived, carried out the experiments and wrote the manuscript; PN carried out the SDS-PAGE, analysed the results, revised and edited the manuscript. M-LT, C-WC, P-PS and C-DD validated and supervised the work. All authors contributed in analysing and improving the manuscript.
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Sharma, V., Tsai, ML., Sun, PP. et al. Sequential ultrasound assisted deep eutectic solvent-based protein extraction from Sacha inchi meal biomass: towards circular bioeconomy. J Food Sci Technol 60, 1425–1434 (2023). https://doi.org/10.1007/s13197-023-05689-0
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DOI: https://doi.org/10.1007/s13197-023-05689-0