Skip to main content

Advertisement

SpringerLink
Log in

Improving phenolic bioactive-linked anti-hyperglycemic functions of dark germinated barley sprouts (Hordeum vulgare L.) using seed elicitation strategy

  • Original Article
  • Published:
Journal of Food Science and Technology Aims and scope Submit manuscript

Abstract

Sprouts of cereal grains, such as barley (Hordeum vulgare L.), are a good source of beneficial phenolic bioactives. Such health relevant phenolic bioactives of cereal sprouts can be targeted to manage chronic hyperglycemia and oxidative stress commonly associated with type 2 diabetes (T2D). Therefore improving phenolic bioactives by stimulating plant endogenous defense responses such as protective pentose phosphate pathway (PPP) during sprouting has significant merit. Based on this metabolic rationale, this study aimed to enhance phenolic bioactives and associated antioxidant and anti-hyperglycemic functions in dark germinated barley sprouts using exogenous elicitor treatments. Dark-germinated sprouts of two malting barley cultivars (Pinnacle and Celebration), treated with chitosan oligosaccharide (COS) and marine protein hydrolysate (GP), were evaluated. Total soluble phenolic content (TSP), phenolic acid profiles, total antioxidant activity (TA) and in vitro inhibitory activities of hyperglycemia relevant α-amylase and α-glucosidase enzymes of the dark germinated barley sprouts were evaluated at day 2, 4, and 6 post elicitor treatments. Overall, TSP content, TA, and α-amylase inhibitory activity of dark germinated barley sprouts decreased, while α-glucosidase inhibitory activity and gallic acid content increased from day 2 to day 6. Among barley cultivars, high phenolic antioxidant-linked anti-hyperglycemic bioactives were observed in Celebration. Furthermore, GP and COS seed elicitor treatments in selective doses improved T2D relevant phenolic-linked anti-hyperglycemic bioactives of barley spouts at day 6. Therefore, such seed elicitation approach can be strategically used to develop bioactive enriched functional food ingredients from cereal sprouts targeting chronic hyperglycemia and oxidative stress linked to T2D.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Ali Asgar M (2013) Anti-diabetic potential of phenolic compounds: a review. Int J Food Prop 16:91–103

    Article  Google Scholar 

  • Andarwulan N, Shetty K (1999) Improvement of pea (Pisum sativum) seed vigour response by fish protein hydrolysates in combination with acetyl salicylic acid. Process Biochem 35:159–165

    Article  CAS  Google Scholar 

  • Babu PVA, Liu D (2008) Green tea catechins and cardiovascular health: an update. Curr Med Chem 15:1840–1850

    Article  CAS  Google Scholar 

  • Baik B, Ullrich SE (2008) Barley for food: characteristics, improvement, and renewed interest. J Cereal Sci 48:233–242

    Article  CAS  Google Scholar 

  • Cervato G, Carabelli M, Gervasio S, Cittera A, Cazzola R, Cestaro B (2000) Antioxdant properties of oregano (Origanum vulgare) leaf extracts. J Food Biochem 24:453–465

    Article  CAS  Google Scholar 

  • du Jardin P (2015) Plant biostimulants: definition, concept, main categories and regulation. Sci Hortic 196:3–14

    Article  Google Scholar 

  • Dykes L, Rooney LW (2007) Phenolic compounds in cereal grains and their health benefit. Cereal Food World 52:105–111

    CAS  Google Scholar 

  • Fowler MJ (2008) Microvascular and macrovascular complications of diabetes. Clin Diabetes 26:77–82

    Article  Google Scholar 

  • Ha KS, Jo SH, Mannam V, Kwon YI, Apostolidis E (2016) Stimulation of phenolics, antioxidant and α-glucosidase inhibitory activities during barley (Hordeum vulgare L.) seed germination. Plant Food Hum Nutr 71:211–217

    Article  CAS  Google Scholar 

  • Hanhineva K, Torronen R, Bondia-Pons I, Pekkinen J, Kolehmainen M, Mykkanen H, Poutanen K (2010) Impact of dietary polyphenols on carbohydrate metabolism. Int J Mol Sci 11:1365–1402

    Article  CAS  Google Scholar 

  • Huang D, Ou B, Hampsch-Woodill M, Flanagan JA, Prior RL (2002) High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format. J Agric Food Chem 50:4437–4444

    Article  CAS  Google Scholar 

  • Hübner F, Arendt EK (2013) Germination of cereal grains as a way to improve the nutritional value: a review. Crit Rev Food Sci Nutr 53:853–861

    Article  Google Scholar 

  • Iacopini P, Baldi M, Storchi P, Sebastiani L (2008) Catechin, epicatechin, quercetin, rutin and resveratrol in red grape: content, in vitro antioxidant activity and interactions. J Food Compos Anal 21:589–598

    Article  CAS  Google Scholar 

  • Inzucchi SE, Bergenstal RM, Buse JB, Diamant M, Ferrannini E, Nauck M, Peters AL, Tsapas A, Wender R, Matthews DR (2015) Management of hyperglycaemia in type 2 diabetes, 2015: a patient-centred approach. Update to a position statement of the American diabetes association and the European association for the study of diabetes. Diabetologia 58:429–442

    Article  Google Scholar 

  • Kähkönen MP, Hopia AU, Vuorela HJ, Rauha J, Pihlaja K, Kujala T, Hainonen M (1999) Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem 47:3957–3962

    Article  Google Scholar 

  • Kahn SE (2003) The relative contributions of insulin resistance and beta-cell dysfunction to the pathophysiology of Type 2 diabetes. Diabetologia 46:3–19

    Article  CAS  Google Scholar 

  • Kakkar S, Bais S (2014) A review on protocatechuic acid and its pharmacological potential. ISRN Pharmacol. doi:10.1155/2014/952943

    Google Scholar 

  • Kwon YI, Vattem DA, Shetty K (2006) Evaluation of clonal herbs of Lamiaceae species for management of diabetes and hypertension. Asia Pac J Clin Nutr 15:107–118

    Google Scholar 

  • Kwon YI, Apsotolidis E, Kim YC, Shetty K (2007) Health benefits of traditional corn, beans, and pumpkin: in vitro studies for hyperglycemia and hypertension management. J Med Food 10:266–275

    Article  CAS  Google Scholar 

  • Lee W, Lee SY, Son YJ, Yun JM (2015) Gallic acid decreases inflammatory cytokine secretion through histone acetyltransferase/histone deacetylase regulation in high glucose-induced human monocytes. J Med Food 18:793–801

    Article  CAS  Google Scholar 

  • Li X, Wang X, Chen D, Chen S (2011) Antioxidant activity and mechanism of protocatechuic acid in vitro. Funct Food Health Dis 1:232–234

    CAS  Google Scholar 

  • Nair SS, Kavrekar V, Mishra A (2013) In vitro studies on alpha amylase and alpha glucosidase inhibitory activities of selected plant extracts. Eur J Exp Biol 3:128–132

    Google Scholar 

  • Oboh G, Ogunsuyi OB, Ogunbadejo MD, Adefegha SA (2016) Influence of gallic acid on α-amylase and α-glucosidase inhibitory properties of acarbose. J Food Drug Anal 24:627–634

    Article  CAS  Google Scholar 

  • Orwat J (2016) Phenolic antioxidant-linked bioactive enrichment in black beans (Phaseolus vulgaris L.) to screen for health benefits and enhancement of salinity resilience. ProQuest Dissertations & Theses. http://gradworks.umi.com/10/15/10150593.html. Accessed 7 Apr 2017

  • Ramakrishna R, Sarkar D, Schwarz P, Shetty K (2017) Phenolic linked anti-hyperglycemic bioactives of barley (Hordeum vulgare L.) cultivars as nutraceuticals targeting type 2 diabetes. Ind Crops Prod. http://dx.doi.org/10.1016/j.indcrop.2017.03.033. (in press)

  • Randhir R, Lin YT, Shetty K (2004) Stimulation of phenolics, antioxidant and antimicrobial activities in dark germinated mung bean sprouts in response to peptide and phytochemical elicitors. Process Biochem 39:637–646

    Article  CAS  Google Scholar 

  • Randhir R, Kwon YI, Shetty K (2009) Improved health-relevant functionality in dark germinated Mucuna pruriens sprouts by elicitation with peptide and phytochemical elicitors. Bioresour Technol 100:4507–4514

    Article  CAS  Google Scholar 

  • Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231–1237

    Article  CAS  Google Scholar 

  • Sarkar D, Shetty K (2014) Metabolic stimulation of plant phenolics for food preservation and health. Annu Rev Food Sci Technol 5:395–413

    Article  CAS  Google Scholar 

  • Shetty K, Wahlqvist M (2004) A model for the role of the proline-linked pentose-phosphate pathway in phenolic phytochemical bio-synthesis and mechanism of action for human health and environmental applications. Asia Pac J Clin Nutr 13:1–24

    CAS  Google Scholar 

  • Shetty K, Curtis OF, Levin R, Ang W (1995) Prevention of vitrification associated with in vitro shoot culture of oregano (Origanum vulgare) by Pseudomonas spp. J Plant Physiol 147:447–451

    Article  CAS  Google Scholar 

  • Stumvoll M, Goldstein BJ, Van Haeften TW (2005) Type 2 diabetes: principles of pathogenesis and therapy. Lancet 365:1333–1346

    Article  CAS  Google Scholar 

  • Trouvelot S, Heloir M, Poinssot B, Gauthier A, Paris F, Guillier C, Combier M, Trda L, Daire X, Adrian M (2014) Carbohydrates in plant immunity and plant protection: roles and potential application as foliar sprays. Front Plant Sci. doi:10.3389/fpls.2014.00592

    Google Scholar 

  • Tundis R, Loizzo MR, Menichini F (2010) Natural products as alpha-amylase and alpha-glucosidase inhibitors and their hypoglycaemic potential in the treatment of diabetes: an update. Mini Rev Med Chem 10:315–331

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Kalidas Shetty

    Present address: Department of Plant Sciences, North Dakota State University, 1320 Albrecht Blvd., 214 Quentin Burdick Building, Fargo, ND, 58102, USA

Authors and Affiliations

  1. Department of Plant Sciences, North Dakota State University, 1320 Albrecht Blvd., 214 Quentin Burdick Building, Fargo, ND, 58102, USA

    Ramnarain Ramakrishna, Dipayan Sarkar, Avani Manduri & Shreyas Ganesan Iyer

Authors
  1. Ramnarain Ramakrishna
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dipayan Sarkar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Avani Manduri
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shreyas Ganesan Iyer
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kalidas Shetty
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kalidas Shetty.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ramakrishna, R., Sarkar, D., Manduri, A. et al. Improving phenolic bioactive-linked anti-hyperglycemic functions of dark germinated barley sprouts (Hordeum vulgare L.) using seed elicitation strategy. J Food Sci Technol 54, 3666–3678 (2017). https://doi.org/10.1007/s13197-017-2828-9

Download citation

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13197-017-2828-9

Keywords

Search

Navigation