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UV-C and hyperoxia abiotic stresses to improve healthiness of carrots: study of combined effects

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Abstract

Phenolic compounds are phytochemicals with high health-promoting properties. Carrot is a vegetable highly worldwide consumed although its phenolic content is low compared to other plant products. The aim of this work was to evaluate changes in phenolic compounds in carrots caused by abiotic stresses. The phenylalanine ammonia-lyase (PAL) activity, phenolic compounds and total antioxidant capacity (TAC) changes during storage up to 72 h at 15 °C after wounding (shredding), 9 kJ UV-C m−2 pretreatment and hyperoxia (80 kPa) conditions of carrots were studied. Shredding and hyperoxia storage induced the highest phenolic compounds and TAC enhancements. Accumulation of phenolic compounds in shredded carrots could be structured in the following phases: 1st phase (<24 h): unchanged phenolic compounds levels with minimum PAL activity; 2nd phase (24–48 h): moderate phenolic increases (≈600–700 mg CAE kg−1 accumulated in 24 h) concurring with the greatest increase of PAL activity; 3nd phase (48–72 h): high phenolic increases (≈1600–2700 mg CAE kg−1, accumulated in 24 h) while a moderate increment of PAL activity was registered. Although UV-C pretreatment of shreds reduced phenolic accumulation, 600 % increments were still registered in those samples stored under hyperoxia conditions for 72 h. However, the contents of chlorogenic acid at 72 h were 1.4-fold higher in irradiated shreds under hyperoxia compared to the same samples under air conditions.

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Acknowledgments

Authors are grateful to the Spanish Ministry Economy and Competitiveness (Project AGL2013-48830-C2-1-R) and FEDER for financial support. We are grateful to V. Díaz-López for his skillful technical assistance.

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Authors and Affiliations

  1. Postharvest and Refrigeration Group, Department of Food Engineering, Universidad Politécnica de Cartagena, Paseo Alfonso XIII, 48, 30203, Cartagena, Murcia, Spain

    Anna Carolina Formica-Oliveira, Ginés Benito Martínez-Hernández, Encarna Aguayo, Francisco Artés & Francisco Artés-Hernández

  2. Institute of Plant Biotechnology, Universidad Politécnica de Cartagena, Campus Muralla del Mar, 30202, Cartagena, Murcia, Spain

    Ginés Benito Martínez-Hernández, Encarna Aguayo, Perla A. Gómez, Francisco Artés & Francisco Artés-Hernández

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  1. Anna Carolina Formica-Oliveira
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  2. Ginés Benito Martínez-Hernández
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  3. Encarna Aguayo
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  4. Perla A. Gómez
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  5. Francisco Artés
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  6. Francisco Artés-Hernández
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Correspondence to Francisco Artés-Hernández.

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Formica-Oliveira, A.C., Martínez-Hernández, G.B., Aguayo, E. et al. UV-C and hyperoxia abiotic stresses to improve healthiness of carrots: study of combined effects. J Food Sci Technol 53, 3465–3476 (2016). https://doi.org/10.1007/s13197-016-2321-x

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  • DOI: https://doi.org/10.1007/s13197-016-2321-x

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