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Subcritical water extraction and antioxidant activity evaluation with on-line HPLC-ABTS·+ assay of phenolic compounds from marigold (Tagetes erecta L.) flower residues

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Abstract

Subcritical water extraction (SWE) of phenolics was investigated from marigold (Tagetes erecta L.) flower residues. The total phenolics content (TPC), total flavonoids content (TFC) and antioxidant capacities of extracts were determined, furthermore, antioxidant activities of individual compounds were evaluated with on-line HPLC–ABTS•+ system. The optimum SWE time was 45 min, solid-to-liquid ratio was 1:50, and the highest TPC and TFC were obtained at 220 °C respectively. The effect of SWE temperature on TPC and TFC was significant (p < 0.05), and TPC was ranged from 28.42 ± 0.94 to 124.27 ± 1.94 (mg GAE/g), and TFC ranged from 34.21 ± 0.36 to 133.22 ± 1.57 (mg GAE/g) between 80 and 220 °C. On-line HPLC–ABTS•+ profiles revealed that quercetagetin from SWE at 200 °C had nearly twofold radical scavenging activities than that by leaching extraction.

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Acknowledgments

This study was funded by Special Program for International S&T Cooperation and Exchanges (CN-SLO 9-21/2011).

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Authors and Affiliations

  1. Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Box 112, No.17 Qinghua East Road, , Haidian District,, Beijing, 100083, China

    Honggao Xu, Weiyou Wang, Junping Jiang, Fang Yuan & Yanxiang Gao

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  1. Honggao Xu
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  2. Weiyou Wang
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  3. Junping Jiang
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  4. Fang Yuan
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  5. Yanxiang Gao
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Correspondence to Yanxiang Gao.

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Xu, H., Wang, W., Jiang, J. et al. Subcritical water extraction and antioxidant activity evaluation with on-line HPLC-ABTS·+ assay of phenolic compounds from marigold (Tagetes erecta L.) flower residues. J Food Sci Technol 52, 3803–3811 (2015). https://doi.org/10.1007/s13197-014-1449-9

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