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Antioxidants and antioxidant enzymes status of rats fed on n-3 PUFA rich Garden cress (Lepidium Sativum L) seed oil and its blended oils

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Abstract

Garden cress (Lepidium sativum L) seed oil (GCO) is a rich source of α-linolenic acid (ALA, 33.6 %) and the oil has a fairly balanced SFA, MUFA and PUFA ratio. In this study we have investigated the effect of GCO and its blends with n-6 PUFA rich edible vegetable oils sunflower oil (SFO), rice bran oil (RBO) and sesame oil (SESO) on antioxidant status of oils and antioxidative enzymes in Wistar rats. Physical blending of GCO with n-6 PUFA rich vegetable oils (SFO, RBO and SESO) increased content of natural antioxidants such as tocopherols, oryzanol and lignans, decreased the n-6/n-3 PUFA ratio and improved the radical scavenging activity of blended oils. Dietary feeding of GCO and its blended oils for 60 days, increased the tocopherols levels (12.2–21.6 %) and activity of antioxidant enzymes namely catalase, glutathione peroxidase (GPx), but did not affect the activity of glutathione reductase (GR), superoxide dismutase (SOD) and glutathione S-transferase (GST) in liver compared to native oil fed rats. Thus, blending of GCO with other vegetable oil decreased n-6/n-3 PUFA ratio (>2.0) and dietary feeding of GCO blended oils increased the antioxidant status and activity of antioxidant enzymes (catalase and GPx) in experimental rats.

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Acknowledgments

Mr. S. S. Umesha, gratefully acknowledges the Council of Scientific and Industrial Research (CSIR) New Delhi, for providing a Senior Research Fellowship. KAN acknowledges the financial support in the form of a Project (SR/SO/HS-0005/2010) awarded by Department of Science and Technology (DST), New Delhi, India.

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  1. Department of Biochemistry and Nutrition, Central Food Technological Research Institute (CSIR), Mysore, 570020, India

    Shankar Shetty Umesha & K. Akhilender Naidu

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  1. Shankar Shetty Umesha
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  2. K. Akhilender Naidu
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Correspondence to K. Akhilender Naidu.

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Umesha, S.S., Naidu, K.A. Antioxidants and antioxidant enzymes status of rats fed on n-3 PUFA rich Garden cress (Lepidium Sativum L) seed oil and its blended oils. J Food Sci Technol 52, 1993–2002 (2015). https://doi.org/10.1007/s13197-013-1196-3

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  • DOI: https://doi.org/10.1007/s13197-013-1196-3

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