Abstract
Gentamicin, an important aminoglycoside, is used to treat many types of bacterial infections, particularly those caused by Gram-negative organisms. It is a nephrotoxic antibiotic, which causes acute tubular necrosis, and its toxicity remains a major problem in clinical use. This study investigates the effect of pomegranate seed oil (PSO) on gentamicin-induced nephrotoxicity in adult male rats. Animals were randomly divided into four groups. Group one was treated with saline (1 ml/kg, i.p.), group 2 received gentamicin 80 mg/kg/day for 6 days and groups 3 and 4 received PSO 0.32 and 0.64 mg/kg/day i.p. respectively, 1 h before gentamicin. Serum urea, creatinine levels, urinary glucose and protein concentrations were evaluated as the markers of acute renal failure. Renal antioxidant indicators such as thiobarbituric acid-reactive substance (TBARS), and total thiol contents, were also determined. A significant elevation of serum creatinine and urea levels as well as urine glucose and protein concentrations were observed in gentamicin treated group. Gentamicin also caused a significant decrease in total thiol content and a significant increase in TBARS levels in kidney homogenate samples. PSO pretreatment resulted in a significant and dose-dependent decrease in serum creatinine and urea levels as well as urine glucose and protein concentrations when compared with gentamicin treated alone. PSO also significantly reversed the gentamicin-induced depletion in total thiol content and elevation in TBARS in kidney homogenate samples. The results of the present study indicate that PSO clearly attenuated gentamicin-induced nephrotoxicity, but elucidation of the mechanism(s) of this protection needs more investigation.
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References
Adams L, Seeram NP, Aggarwal BB (2006) Pomegranate juice, total pomegranate tannins and punicalagin suppress inflammatory cell signaling in colon cancer cells. J Agric Food Chem 54:980–985
Boroushaki MT, Sadeghnia HR (2009) Protective effect of safranal against gentamicin-induced nephrotoxicity in rat. Iran J Med Sci 34:285–288
Boroushaki MT, Sadeghnia HR, Banihasan M (2010) Protective effect of pomegranate seed oil on hexacholorobutadiene -induced nephrotoxicity in rat. J Ren Fail 32:612–617
Braga LC, Shupp JW, Cummings C (2005) Pomegranate extract inhibits staphylococcus aureus growth and subsequent enterotoxin production. J Ethnopharmacol 96:335–339
Fernandez J, Perez-Alvarez JA, Fernandez-lopez JA (1997) Thiobarbituric acid test for monitoring lipid oxidation in meat. J Food Chem 99:345–353
Gurib-Fakim A (2006) Medicinal plants: traditions of yesterday and drugs of tomorrow. J Mol Aspects Med 27:1–93
Hosseinzadeh H, Sadeghnia HR, Ziaee T (2005) Protective effect of aqueous saffron extract (Crocus sativus L.) and crocin, its active constituent, on renal ischemia-reperfusion induced oxidative damage in rats. J Pharm Sci 8:387–393
Jurenka J (2008) Therapeutic applications of pomegranate (Punicagranatum L.): a review. J Altern Med Rev 13:128–144
Kaufman M, Wiesman Z (2007) Pomegranate oil analysis with emphasis on MALDI-TOF/MS triacylglycerol fingerprinting. J Agric Food Chem 55:10405–10413
Kawaii S, Lansky EP (2004) Differentiation-promoting activity of pomegranate (Punica granatum) fruit extracts in HL-60 human promyelocytic leukemia cells. J Med Food 7:13–18
Khan N, Hadi N, Afaq F et al (2007) Pomegranate fruit extract inhibits prosurvival pathways in human A549 lung carcinoma cells and tumor growth in athymic nude mice. J Carcinogenesis 28:163–173
Kim ND, Mehta R, Yu W (2002) Chemopreventive and adjuvant therapeutic potential of pomegranate (Punica granatum) for human breast cancer. J Breast Cancer Res Treat 71:203–217
Kohno H, Suzuk R, Yasui Y (2004) Pomegranate seed oil rich in conjugated linolenic acid suppresses chemically induced colon carcinogenesis in rats. J Cancer Sci 95:481–486
Lansky P, Newman RA (2007) Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. J Ethnopharmacol 109:177–206
Lott JA, Turner K (1975) Evaluation of Trinder’s glucose oxidase method for measuring glucose in serum and urine. J Clin Chem 21:1754–1760
Masson P, Ohlsson P, Bjorkhem I (1981) Combined enzymatic-Jaffe’s method for determination of creatinine in serum. J Clin Chem 27:18–21
Mc Elderry LA, Tarbit IF, Cassells-Smith AJ (1982) Six methods for urinary protein compared. J Clin Chem 28:356–360
Menezes SM, Cordeiro LN, Viana GS (2006) Punica granatum (pomegranate) extract is active against dental plaque. J Herb Pharmacother 6:79–92
Rasheed Z, Akhtar N, Anbazhagan AN (2009) Polyphenol-rich pomegranate fruit extract (POMx) suppresses PMACI-induced expression of proinflammatory cytokines by inhibiting the activation of MAP kinases and NF-kappaB in human KU812 cells. J Inflamm 6:1–30
Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. J Anal Biochem 25:192–205
Sundin DP, Sandoval R, Molitoris BA (2001) Gentamicin inhibits renal protein and phospholipid metabolism in rats: implications involving intracellular trafficking. J Am Soc Nephrol 12:114–123
Vivancos M, Moreno JJ (2005) Beta-sitosterol modulates antioxidant enzyme response in RAW 264.7 macrophages. J Free Radic Biol Med 39:91–97
Yamasaki M, Kitagawa T, Koyanagi N (2006) Dietary effect of pomegranate seed oil on immune function and lipid metabolism in mice. J Nutrition 22:54–59
Ziegler DM (1985) Role of reversible oxidation-reduction of enzyme thiols-disulfides in metabolic regulation. J Ann Rev Biochem 54:305–329
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This investigation was financially supported by the Vice Presidency in Research, Mashhad University of Medical Sciences, Mashhad, Iran.
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Boroushaki, M.T., Asadpour, E., Sadeghnia, H.R. et al. Effect of pomegranate seed oil against gentamicin -induced nephrotoxicity in rat. J Food Sci Technol 51, 3510–3514 (2014). https://doi.org/10.1007/s13197-012-0881-y
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DOI: https://doi.org/10.1007/s13197-012-0881-y