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Rodent Leukocyte Isolation and Radiolabeling for Inflammation Imaging Study

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Nuclear Medicine and Molecular Imaging Aims and scope Submit manuscript

Abstract

Purpose

The objective of this study was to describe to develop methods of rodent leukocyte isolation and radiolabeling for in vivo inflammation imaging.

Methods

Thigh muscle inflammation was induced by injection of collagenase. Blood was collected from the jugular vein and separated by Histopaque. The collected cells were incubated in a 37 °C CO2 incubator for 1~2 h. After incubation, 99mTc-HMPAO and 18F-FDG were used to treat leukocytes followed by incubation for 30 min. 99mTc-HMPAO and 18F-FDG labeled autologous leukocytes were injected into the tail veins of rats. The images were then acquired at various time points. Image-based lesion to normal muscle ratio was compared.

Results

After Histopaque separation, the proportion of lymphocytes was higher than that of other cell types. After CO2 incubation, the collected leukocytes were viable, while room temperature exposed leukocytes without CO2 incubation were non-viable. Granulocytes, especially, were more quickly influenced by various conditions than the mononuclear cells. Labeling efficiencies of 99mTc-HMPAO and 18F-FDG were 4.00 ± 2.06 and 1.8%, respectively. 99mTc-HMPAO- and 18F-FDG-labeled leukocytes targeted well the inflamed lesion. 99mTc-HMPAO-labeled leukocytes, but not 18F-FDG-labeled leukocytes, were found in the abdomen activity.

Conclusion

Inflamed lesions of rats were well visualized using autologous radiolabeled leukocytes. This method might provide good information for understanding inflammatory diseases.

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Funding

This paper was supported by a Fund of Biomedical Research Institute, Jeonbuk National University Hospital (2019) and a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (grant number : HI18C0139).

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Authors and Affiliations

  1. Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Research Institute of Clinical Medicine of Jeonbuk National University and Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju, Jeonbuk, Republic of Korea

    Eun-Mi Kim, Phil-Sun Oh, Fatima Boud, Hwan-Jeong Jeong, Seok-Tae Lim & Myung-Hee Sohn

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  1. Eun-Mi Kim
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Correspondence to Hwan-Jeong Jeong.

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Conflict of Interest

Eun-Mi Kim, Fatima Boud, Phil-Sun Oh, Hwan-Jeong Jeong, Seok Tae Lim and Myung-Hee Shon certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript

Ethical Approval

All procedures performed in studies involving animals were in accordance with the ethical standards of the institutional research committee. This article does not contain any studies with human participants performed by any of the authors

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The requirement to obtain informed consent was waived in this non-human-subject study.

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Kim, EM., Oh, PS., Boud, F. et al. Rodent Leukocyte Isolation and Radiolabeling for Inflammation Imaging Study. Nucl Med Mol Imaging 54, 147–155 (2020). https://doi.org/10.1007/s13139-020-00645-8

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  • DOI: https://doi.org/10.1007/s13139-020-00645-8

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