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CGRP 8-37 enhances lipopolysaccharide-induced acute lung injury and regulating aquaporin 1 and 5 expressions in rats

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An Erratum to this article was published on 05 October 2017

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Abstract

Calcitonin gene-related peptide (CGRP) has been shown to play important roles in biological functions. However, there is very little evidence on the value of CGRP in lipopolysaccharide (LPS)-induced acute lung injury/acute respiratory distress syndrome (ALI/ARDS). Therefore, this study aimed to investigate the role of CGRP in LPS-induced ALI in rats. In the experiment, Sprague-Dawley (SD) rats were randomized into control, an antagonist of α-calcitonin gene-related peptide receptor (CGRP8-37), LPS groups, and CGRP8-37 + LPS groups. ALI model was prepared through retrograde injection of LPS (10 mg/kg). At 6 and 12 h, bronchoalveolar lavage was performed and used to assess total cell count and levels of tumor necrosis factor-α, interleukin-1β, -6, and -10 by enzyme-linked immunosorbent assay (ELISA). Lung tissue was collected for assessing wet-to-dry (W/D) ratio, hematoxylin and eosin staining. Aquaporin (AQP)-1 and -5 expressions in lung tissues were detected by quantitative PCR and Western blot. The results showed that histological injury, total cell count, and W/D ratio significantly reduced in LPS group after 6 h. The levels of inflammatory cytokines in CGRP8-37 + LPS-treated rats were higher than that in LPS-treated rats (all, P < 0.001). Real-time RT-PCR analysis showed that levels of AQP-1 in rats from CGRP8-37 + LPS group was lower than that in LPS-treated rats (P = 0.005 and P < 0.001). Western blotting analysis showed that AQP-1 protein levels at 6 h significantly decreased in CGRP8-37 + LPS rats. Together, our data suggest that CGRP antagonists, CGRP8-37 could enhance ALI induced by LPS in the rat model, and regulate the expression levels of AQP-1 and AQP-5 by affecting inflammatory cytokines. Thereby, regulating endogenous CGRP may be a potential treatment for ALI/ARDS.

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Change history

  • 05 October 2017

    Volume 73 issue 3 was published with an incorrect cover date. Correct is August 2017. The Publisher apologizes for this mistake and all related inconveniences caused by this.

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Acknowledgments

This work was supported by the Grant #81260289 from the National Natural Science Foundation of China and the Grant #2013FB194 from the Applied Basic Research Foundation of Yunnan Province, China.

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Authors and Affiliations

  1. Comprehensive Pediatric Internal Department, Children’s Hospital, Kunming Medical University, Kunming, 6500032, People’s Republic of China

    Fu Hong-min

  2. Department of Pediatrics, The first people’s hospital of Yunnan province, Kunming, China

    Huangfu Chun-rong, Zheng Rui, Su Li-na, Wang Ya-jun & Li Li

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  1. Fu Hong-min
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  2. Huangfu Chun-rong
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  4. Su Li-na
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Correspondence to Li Li.

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An erratum to this article is available at https://doi.org/10.1007/s13105-017-0593-x.

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Hong-min, F., Chun-rong, H., Rui, Z. et al. CGRP 8-37 enhances lipopolysaccharide-induced acute lung injury and regulating aquaporin 1 and 5 expressions in rats. J Physiol Biochem 73, 381–386 (2016). https://doi.org/10.1007/s13105-017-0563-3

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