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Depot-specific regulation of autotaxin with obesity in human adipose tissue

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Abstract

Autotaxin (ATX) is a lysophospholipase D involved in synthesis of a bioactive mediator: lysophosphatidic. ATX is abundantly produced by adipocytes and exerts a negative action on adipose tissue expansion. In both mice and humans, ATX expression increases with obesity in association with insulin resistance. In the present study, fat depot-specific regulation of ATX was explored in human. ATX mRNA expression was quantified in visceral and subcutaneous adipose tissue in obese (BMI > 40 kg/m2; n = 27) and non-obese patients (BMI < 25 kg/m2; n = 10). Whatever the weight status of the patients is, ATX expression was always higher (1.3- to 6-fold) in subcutaneous than in visceral fat. Nevertheless, visceral fat ATX was significantly higher (42 %) in obese than in non-obese patients, whereas subcutaneous fat ATX remained unchanged. In obese patients, visceral fat ATX expression was positively correlated with diastolic arterial blood pressure (r = 0.67; P = 0.001). This correlation was not observed with subcutaneous fat ATX. Visceral fat ATX was mainly correlated with leptin (r = 0.60; P = 0.001), inducible nitric oxide synthase (r = 0.58; P = 0,007), and apelin receptor (r = 0.50; P = 0.007). These correlations were not observed with subcutaneous fat ATX. These results reveal that obesity-associated upregulation of human adipose tissue ATX is specific to the visceral fat depot.

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Acknowledgments

This work was supported by grants from the INSERM and the Fondation pour la Recherche Médicale (grant #DRM20101220459). Chloé Rancoule and Rodolphe Dusaulcy were supported by a grant from the Ministère de l'Education Nationale de la Recherche et de la Technologie (France).

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Authors and Affiliations

  1. Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Inserm U1048, Université Paul Sabatier, Equipe n°3 (Bat L4), 1 Avenue Jean Poulhès, BP 84225, 31432, Toulouse Cedex 4, France

    Chloé Rancoule, Rodolphe Dusaulcy, Karine Tréguer, Sandra Grès, Charlotte Guigné, Philippe Valet & Jean Sébastien Saulnier-Blache

  2. Service de Diabétologie, Maladies Métaboliques et Nutrition, Hôpital J. d’Arc, CHU de Nancy, Nancy, France

    Didier Quilliot

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  1. Chloé Rancoule
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  2. Rodolphe Dusaulcy
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  3. Karine Tréguer
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  4. Sandra Grès
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  5. Charlotte Guigné
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  8. Jean Sébastien Saulnier-Blache
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Correspondence to Jean Sébastien Saulnier-Blache.

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Rancoule, C., Dusaulcy, R., Tréguer, K. et al. Depot-specific regulation of autotaxin with obesity in human adipose tissue. J Physiol Biochem 68, 635–644 (2012). https://doi.org/10.1007/s13105-012-0181-z

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  • DOI: https://doi.org/10.1007/s13105-012-0181-z

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